1991

Daily life of an AIDS patient. [videorecording]: Princeton, N.J. : Films for the Humanities, c1987 1 videocassette : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9100722A; Hyman H
Just a regular kid : an AIDS story. [videorecording]: [United States] : Diana Kerew Productions, c1987 1 videocassette : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9100754A; Henderson F; (Producer) This program edited for educational use, puts the critical need for AIDS education before the teenage audience. With concern for the spread of AIDS crossing into all segments of society, it is important to know how the disease is transmitted. This drama presents important information on AIDS and confronts
Treating and preventing AIDS. [videorecording]: Princeton, N.J. : Films for the Humanities, c1988 1 videocassette : sd., col. ; 1/2 in (Facts for life) Unique Identifier : AIDSLINE AV/9100762A; (Producer) AIDS leaves its victim with no defenses against infection. In this very frank program, a heterosexual drug user describes life with AIDS, as doctors seek delaying tactics, treating the infections to which victims are prone. AIDS victims make clear recommendations to those who still have a choice: proper u
AIDS. [videorecording]: Bloomington, MN : Health Education Video, [1989] 1 videocassette (12 min., 34 sec.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9100765A
6th International Conference on AIDS, San Francisco. [sound recording]: North Hollywood, CA : Professional Programs, 1990 121 sound cassettes : 1 7/8 ips Unique Identifier : AIDSLINE AV/9100685A
AIDS in Africa. [videorecording]: [Canada] : Roger Pyke Productions, c1990 1 videocassette (51 min., 35 sec.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9100716A; (Producer) This documentary is the most comprehensive report from the frontlines of the war on AIDS. The disease is especially rampant in Central Africa where it is estimated that several million may be dead by the year 2000. Unlike Europe and the United States , where 90% of AIDS vi
AIDS : ABC News special assignment. [videorecording]: Deerfield, IL : MTI Film & Video, [1990] 1 videocassette (13 min., 21 sec.) : sd., col. ; 1/2 in. + 1 leaflet Unique Identifier : AIDSLINE AV/9100751A; (Producer) This program gives the most recent facts about AIDS: where it came from, how it is spread, what is being done to combat it. This program helps fight some of the fear-inspired myths about AIDS and leads to a more realistic and compassionate understanding of the disease and its victims.
The:Family physician's guide to HIV infection and AIDS. [videorecording]: Kansas City, Mo. : American Academy of Family Physicians, c1990 1 videocassette : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9100753A; Tudor JM; Felmar EA
AIDS/HIV clinical trial handbook.: New York, N.Y. (Suite T-3601 1515 Broadway, New York 10036) : American Foundation for AIDS Research, [between 1985 and 1991] 20 p Unique Identifier : AIDSLINE CAT/9106886
AIDS : facts and fiction.: Accra : Ghana Universities Press, 1986 30 p Unique Identifier : AIDSLINE CAT/9106098; Neequaye AR
The:AIDS/HIV epidemic.: [St. Paul, Minn. : The Dept., 1988] 92 p. : ill Unique Identifier : AIDSLINE CAT/9106100
Drug-nutrient interactions in AIDS guidebook.: Fremont, Calif. : Cutting Edge, c1988 58, 24, 11 p Unique Identifier : AIDSLINE CAT/9107798; Newman CF; Horn B
[El:SIDA en Mexico : los efectos sociales.] 1. ed.: [Mexico] : Ediciones de Cultura Popular, 1988 399 p. : ill Unique Identifier : AIDSLINE CAT/9107680; Galvan Diaz F
AIDS treatment research and approval : hearing before the Committee on Labor and Human Resources, United States Senate, One Hundredth Congress, second session, on examining what progress has been made, what is needed to make progress more rapid, and what barriers stand in the way of better research and swifter distribution of hopeful therapies, July 13, 1988.: Resources Washington : U.S. G.P.O. ; For sale by the Supt. of Docs., Congressional Sales Office, U.S. G.P.O., 1988 iii, 198 p. : ill (S. hrg. ; 100-821) Unique Identifier : AIDSLINE CAT/9107742
[AIDS : powstawanie, obraz kliniczny, proby leczenia, epidemiologia.]: Wroclaw : Zaklad Narodowy im. Ossolinskich, 1988 181 p. : ill Unique Identifier : AIDSLINE CAT/9107743; Skotnicki AB
[AIDS : een nieuwe verantwoordelijkheid voor gezondheidszorg en onderwijs.]: Amsterdam : Swets & Zeitlinger, c1989 200 p. : ill Unique Identifier : AIDSLINE CAT/9106099; Noordhof A
[AIDS, medisch en sociaal : een evaluatie van een TELEAC-cursus.]: Delft : Eburon, 1989 123 p. : ill (NISSO studies (nieuwe reeks) ; nr. 1) Unique Identifier : AIDSLINE CAT/9106101; Lodewijkx H
[Diagnostiek en behandeling van patienten met een HIV-infectie : een protocollaire benadering.]: Utrecht : Bohn, Scheltema & Holkema, 1989 63 p Unique Identifier : AIDSLINE CAT/9106166; Danner SA
[Schwarze Angst, Leben mit AIDS.] 1. Aufl.: Stuttgart : Quell, 1989 240 p. : ill Unique Identifier : AIDSLINE CAT/9106778; Becker-Kolle C
[SIDA en Colombia : nunca me imagine que podria infectarme testimonios.] 2. ed.: [Colombia? : s.n.], 1989. -- (Medellin, Colombia : Editorial Colina) 283 p. : ill Unique Identifier : AIDSLINE CAT/9106791; Gomez Gomez S
Public and professional attitudes toward AIDS patients : a national dilemma.: Boulder : Westview Press, 1989 viii, 136 p Unique Identifier : AIDSLINE CAT/9107174; Rogers DE; Ginzberg E
Education for the prevention and control of AIDS.: Bangkok : Unesco Principal Region Office for Asia and the Pacific, 1989 iii, 121 p (Abstract-bibliography series ; 10) Unique Identifier : AIDSLINE CAT/9106951
AIDS and young people.: Eastbourne : Kingsway Publications, 1989 127 p Unique Identifier : AIDSLINE CAT/9107358; Dixon P
AIDS education, care, and drug development : hearing before the Committee on Labor and Human Resources, United States Senate, One Hundred First Congress, first session, on examining the adequacy of the federal government efforts to combat AIDS, with regard to education, care, and drug development, February 7, 1989.: Resources Washington : U.S. G.P.O. ; For sale by the Supt. of Docs., Congressional Sales Office, U.S. G.P.O., 1989 iv, 219 p. : ill (S. hrg. ; 101-22) Unique Identifier : AIDSLINE CAT/9107359
[Sida espoir : ce que nous apprennent les vainqueurs du sida.]: Chene-Bourg, Geneve : Editions Soleil, c1990 235 p. : ill Unique Identifier : AIDSLINE CAT/9106782; Docteur Soleil
AIDS litigation project : a national survey of federal, state, & local cases before courts & human rights commissions.: Washington, DC : Dept. of Health and Human Services, Public Health Service, Office of the Assistant Secretary for Health, Office of PHS HIV/AIDS Coordination, 1990 viii, 172 p Unique Identifier : AIDSLINE CAT/9106884; Gostin LO; Porter L; Sandomire H; Gostin LO
Resources for teaching about the social impact of AIDS in Africa.: Bloomington, IN : African Studies Program, Indiana University, [1990] 24 p Unique Identifier : AIDSLINE CAT/9106761; Schmidt NJ
[AIDS und Sozialleistungen : Leitfaden fur Betroffene und Betreuer bei HIV-Infektion und AIDS-Erkrankung.] 1. Aufl.: Sankt Augustin : Asgard-Verlag, 1990 112 p Unique Identifier : AIDSLINE CAT/9107360; Lichtenberg P
[Tiempos del SIDA : relatos de la vida real.]: San Jose, Costa Rica : Euroamericana de Ediciones, 1990 printing 117 p Unique Identifier : AIDSLINE CAT/9107244; Francis M
[Le:S.I.D.A.] 3. ed. mise a jour.: Paris : Presses universitaires de France, 1990 127 p. : ill (Que sais-je? ; 2332) Unique Identifier : AIDSLINE CAT/9107191; Cassuto J
Providers of health care services, 1984/85-1987/88.: [Sacramento, Calif.] : Dept. of Health Services, [1990] ii, 16 p. : ill (Medi-Cal studies in AIDS ; no. 3) Unique Identifier : AIDSLINE CAT/9107167
AIDS and ethics : a bibliography.: Services Silver Spring, MD : National Center for Social Policy & Practice, Information Services, c1990 Unique Identifier : AIDSLINE CAT/9107740; The National Center for Social Policy & Practice created this bibliography about AIDS and ethics as a balanced and extensive source of current published literature on the range of issues pertaining to the ethical dimensions of AIDS. Citations were derived from searches of 21 databases from various fields, such as s
Cancer and AIDS : a psychogenic theory and case report.: Frankfurt am Main : Haag + Herchen, c1990 375 p. : ill Unique Identifier : AIDSLINE CAT/9107763; Simmons HE
AIDS/HIV treatment directory. Vol. 4, no. 1 (June 1990)-: New York, NY : The Foundation, c1990- v. : ill Unique Identifier : AIDSLINE CAT/9107362
[Ergebnisse sozialwissenschaftlicher Aids-Forschung.] Bd. 1-: Berlin : Edition Sigma, c1990- v. : ill Unique Identifier : AIDSLINE CAT/9107450
Pediatric AIDS and HIV infection. Vol. 1, no. 1-: New York, NY : Mary Ann Liebert, Inc., c1990- v. : ill Unique Identifier : AIDSLINE CAT/9107942
Genetic structure and regulation of HIV.: New York : Raven Press, c1991 xix, 537 p. : ill (Harvard AIDS Institute series on gene regulation of human retroviruses ; v. 1) Unique Identifier : AIDSLINE CAT/9106203; Haseltine WA; Wong-Staal F
AIDS practice manual : a legal and educational guide. 3rd ed.: San Francisco, CA : National Lawyers Guild AIDS Network in conjunction with AIDS Law Project of Pennsylvania, Gay Men's Health Crisis, Whitman-Walker Clinic, c1991 1 v. (various pagings) Unique Identifier : AIDSLINE CAT/9106522; Albert P
AIDS.: New York : Facts on File, c1991 viii, 248 p (Library in a book) Unique Identifier : AIDSLINE CAT/9106885; Flanders SA; Flanders CN; Discusses the AIDS epidemic, including a chronology of major events, a summary of important court cases, a biographical listing of key individuals, and a reference guide to further research.
A:Community approach to AIDS intervention : exploring the Miami outreach project for injecting drug users and other high risk groups.: New York : Greenwood Press, 1991 xii, 234 p. : ill (Contributions in medical studies, ISSN 0886-8220 ; no. 33) Unique Identifier : AIDSLINE CAT/9106551; Chitwood DD
AIDS : abstracts of the psychological and behavioral literature, 1983-1991. 3rd ed.: Arlington, Va. : American Psychological Association, c1991 ix, 301 p (Bibliographies in psychology ; no. 6) Unique Identifier : AIDSLINE CAT/9107744; Anderson JR; Landry CP; Kerby JL
HIV testing & quality control : a guide for laboratory personnel.: Durham, N.C., USA : AIDSTECH, Family Health International, 1991 xii, 165 p. : ill., forms Unique Identifier : AIDSLINE CAT/9107500; Constantine NT; Callahan JD; Watts DM; Mitchell S; Gringle R; Archbold E
Prospects for antisense nucleic acid therapy of cancer and AIDS.: New York : Wiley-Liss, c1991 xiv, 269 p. : ill Unique Identifier : AIDSLINE CAT/9107966; Wickstrom E
Neuropathology and ophthalmologic pathology of the acquired immunodeficiency syndrome : a color atlas.: East Hanover, N.J. : Sandoz ; Boston : Butterworth-Heinemann, c1992 ix, 165 p. : ill Unique Identifier : AIDSLINE CAT/9107578; De Girolami U
AIDS prevention and treatment : hope, humor, and healing.: New York : Hemisphere Pub. Corp., c1992 xiii, 276 p. : ill (The Series in health psychology and behavioral medicine, ISSN 8756-467X) Unique Identifier : AIDSLINE CAT/9107741; Seligson MR; Peterson KE
HIV/AIDS : a guide to nursing care. 2nd ed.: Philadelphia : Saunders, c1992 xiii, 515 p. : ill Unique Identifier : AIDSLINE CAT/9107501; Flaskerud JH; Ungvarski PJ
AIDS : an African perspective.: Boca Raton : CRC Press, c1992 363 p. : ill Unique Identifier : AIDSLINE CAT/9107361; Williams AO
EPSTEIN-BARR VIRUS (EBV) GENOTYPES IN AIDS-ASSOCIATED LYMPHOMAS ARE SIMILAR TO THOSE IN ENDEMIC BURKITT'S LYMPHOMAS (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1320 1991. Unique Identifier : AIDSLINE ICDB/91674135; Goldschmidts W; Bhatia K; Gutierrez M; Carolan M; Akar N; Magrath I; Pediatric Branch, NCI, Bethesda, MD 20892; Recent data have suggested that both the major EBV subtypes (type A and type B) are equally prevalent in the US. This is in contrast to the infrequent association of the type B virus in sporadic EBV-positive Burkitt s tumors. To characterize the distribution of the two EBV strains in EBV-associated neoplasms, we scree
ANTISENSE-MEDIATED SPECIFIC INHIBITION OF P120 PROTEIN EXPRESSION AND CELL PROLIFERATION (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1642 1991. Unique Identifier : AIDSLINE ICDB/91674438; Fonagy A; Wilson A; Busch H; Freeman JW; Dept. of Surgery, Univ. of Kentucky Medical Center, Lexington, KY; 40536; Monoclonal antibodies, cDNAs, and the genomic DNA have been obtained for the proliferation-associated nucleolar antigen P120 (Freeman et al, Cancer Res 48:1244-51, 1988; Fonagy et al, Cancer Comm 1:243-51, 1989; and Larson et al, Cancer Comm 2:63-71, 1990). In this study, we report that transfection of a pentadecadeox
PHORBOL DIESTER STIMULATED HL-60 CELL DIFFERENTIATION IS ASSOCIATED WITH THE ACTIVATION OF SPECIFIC DNA BINDING PROTEINS (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1751 1991. Unique Identifier : AIDSLINE ICDB/91674552; Rogers M; Small GW; Samples L; Daniel LW; Dept. of Biochemistry, Bowman Gray Sch. of Medicine, Wake Forest; Univ., Winston-Salem, NC 27103-2796; TPA-stimulated differentiation of HL-60 cells to macrophagelike cells is accompanied by morphological and biochemical changes, including cellular adhesion and expression of the lymphocyte cell adhesion molecule subunit cd 11b. Using cellular adhesion assays and direct immunofluorescence microscopy of cd 11b, we observ
MUTATIONS INTRODUCED ALONG THE HTLV-1 ENVELOPE GENE RESULT IN A NONFUNCTIONAL PROTEIN: A BASIS FOR ENVELOPE CONSERVATION (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1846 1991. Unique Identifier : AIDSLINE ICDB/91674648; Dokhelar MC; Pique C; Tursz T; Institut G. Roussy, Villejuif, France; The envelope protein of the human T leukemia virus type I (HTLV-I) is highly conserved among the isolates sequenced so far, as opposed to what is observed for the human immunodeficiency virus envelope. By linker insertion scanning, we have produced 33 random mutations along the HTLV-I envelope gene, cloned into a euka
HUMAN GLIAL CELLS FROM FETAL BUT NOT ADULT HUMAN BRAIN ACTIVATE THE EXPRESSION OF HIV IN CHRONICALLY INFECTED MONOCYTES (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1860 1991. Unique Identifier : AIDSLINE ICDB/91674662; Kalebic T; Helman L; Pizzo P; Lab. of Molecular Genetics, Pediatric Branch, NCI, Bethesda, MD; 20892; The purpose of this study was to investigate whether human glial cell lines derived from fetal brain release soluble factors which induce the expression of latent HIV in chronically infected monocytes. As a cellular model for HIV latency, we used the promonocytic U1 cell line, harboring in its genome 2 copies of provi
IMMUNOELECTRON MICROSCOPIC STUDY OF HTLV-I-PRODUCING HUMAN LYMPHOCYTES IN VITRO (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1861 1991. Unique Identifier : AIDSLINE ICDB/91674663; Ohtsuki Y; Iwata J; Oka T; Furihata M; Sonobe H; Miyoshi I; Dept. of Pathology, Kochi Medical Sch., Kochi 783, Japan; HTLV-I-producing human lymphoid cell lines, derived from ATL and HAM patients, and lymphocytes from HTLV-I carriers and seronegative healthy individuals were tested for lymphocyte markers, on the viral envelope and the plasma membrane. Short-term cultured lymphocytes from HTLV-I carriers, but not the virus were labele
RESPONSE OF CALVES INOCULATED WITH BOVINE IMMUNODEFICIENCY-LIKE VIRUS (BIV) (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A1862 1991. Unique Identifier : AIDSLINE ICDB/91674664; Thurmond M; Theilen G; Stott J; Kwon O; Sninsky J; Koyama M; Univ. of California, Davis, CA 95616; Calves were inoculated with BIV to determine pathogenesis of this virus under controlled conditions. They were frequently examined for viremia, presence of antibodies to BIV and immunologic changes. Presence of viremia was monitored in vivo by PCR and in vitro by RT assays and syncytia formation. Antibody titers were
THE OXYGEN FREE RADICAL-ASSOCIATED DNA ADDUCT 8-OXOGUANINE CAUSES G-C TO T-A TRANSVERSIONS DURING IN VIVO DNA REPLICATION (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 32:A895 1991. Unique Identifier : AIDSLINE ICDB/91673722; Cheng KC; Cahill DS; Kasai H; Nishimura S; Loeb LA; Dept. of Pathology, Univ. of Washington, Seattle, WA 98195; A major product of oxygen damage to DNA is 8-oxoguanine. The mutagenic potential of this adduct was tested in the M13G assay, a site-specific assay for in vivo mutations caused by guanine adducts inserted into the E coli lacZ gene. Site-specific insertion of 8-oxoguanine using the DNA polymerase activity of HIV-1 reve
Molecular diversity of SIVsm/PBj and a cognate variant (SIVsm/PGg).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:17 (abstract no. 1). Unique Identifier : AIDSLINE PRIM8/900001; Novembre FJ; Hirsch VM; McClure HM; Johnson PR; Department of Microbiology, Georgetown University, and Laboratory; of Infectious Diseases, NIAID/NIH, Rockville, Md.; Experimental inoculation of SIVsm/PBj (PBj) into pig-tail macaques induces an acute syndrome characterized by fulminant diarrhea, acid-base imbalance, and death. This outcome is in distinct contrast to the more protracted immunodeficiency syndrome (AIDS) caused by other SIVsm isolates, including the PBj parental strai
Both in vitro culture and in vivo passage lead to selective replication of SIV variants.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:18 (abstract no. 2). Unique Identifier : AIDSLINE PRIM8/900002; Villinger F; De B; Jehuda-Cohen T; Powell JD; Neckelmann N; McClure HM; Folks TM; Ansari AA; Yerkes Regional Primate Research Center, Dept. of Pathology,; Emory University School of Medicine, Atlanta, GA 30322; A set of oligo primers was prepared based on the sequence shared by various SIV isolates from a variety of nonhuman primate species. This primer pair was used to amplify and sequence the middle third of the gag region of the endogenous SIVsmm (SIVsmm-e) present in the DNA prepared from the PBMC of naturally infected c
The fate of molecularly cloned SIV in vivo: patterns of genetic evolution associated with AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:19 (abstract no. 3). Unique Identifier : AIDSLINE PRIM8/900003; Johnson PR; Hamm TE; Hirsch VM; Department of Microbiology, Georgetown University, and Laboratory; of Infectious Diseases, NIAID/NIH, Rockville, Md. 20852; One potential obstacle to the development of an effective AIDS vaccine is the inordinate genetic heterogeneity of HIV isolates. The basis for this genetic diversity appears to be the extreme plasticity of the HIV genome. Although highly informative, studies presented to date on the genetic variation of HIV-1 from infe
Genetic determinants of SIVmac macrophage tropism map to env.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:20 (abstract no. 4). Unique Identifier : AIDSLINE PRIM8/900004; Mori K; Sehgal P; Ringler D; Desrosiers R; New England Regional Primate Research Center; A molecular clone of SIVmac239 produces virus which causes AIDS in rhesus monkeys in a time frame suitable for laboratory investigation. This cloned virus replicates well in primary PBL cultures and in CD4+ lymphoid cell lines but it replicates poorly in cultured macrophages. We have used primary alveolar macrophages
Nucleotide sequence of the pathogenic SIV239 clone and analysis of nef function.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:21 (abstract no. 5). Unique Identifier : AIDSLINE PRIM8/900005; Kestler H; Regier D; Mori K; Kodama T; Daniel M; Desrosiers R; Department of Microbiology and Molecular Genetics, New England; Regional Primate Research Center, Harvard Medical School,; Southborough, MA, USA; The SIVmac239 infectious molecular clone yields virus that induces AIDS in rhesus monkeys in a time frame suitable for laboratory investigation. The proviral genome, including both LTRs, is 10,279 base pairs. Nine open reading frames, previously identified in other HIV-2/SIVmac/SIVsmm isolates, were also present in th
Biological characterization of SIV molecular clones from tissue of an immunodeficient macaque.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:22 (abstract no. 6). Unique Identifier : AIDSLINE PRIM8/900006; Hirsch VM; Dapolito G; Goldstein S; Johnson PR; Department of Microbiology, Georgetown University and Laboratory; of Infectious Diseases, NIAID, NIH, Rockville, MD; Tissues of immunodeficient macaques experimentally infected with SIVsm contain multiple diverse genotypes, best described as a proviral swarm. We wished to assess the biologic relevance of these multiple genomes. For this purpose, we used PCR to derive molecular clones of the 3 half (4.3 kb) of the proviral genome fr
Molecular analysis of SIV from stumptailed macaque indicates two distinct origins of infection of captive monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:23 (abstract no. 7). Unique Identifier : AIDSLINE PRIM8/900007; Khan AS; Galvin T; Gardner MB; Lowenstine LJ; NIAID, NIH, Bethesda, MD; The natural hosts of simian immunodeficiency viruses (SIVs) are African monkeys including sooty mangabeys, mandrills and African green monkeys. SIVs, however, have also been isolated from captive Asian macaques such as pigtailed, rhesus and stumptailed macaques. The Asian monkeys are thought to have acquired SIVs in c
Natural infection of West African green monkeys with a unique SIVAGM subtype.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:24 (abstract no. 8). Unique Identifier : AIDSLINE PRIM8/900008; Allan JS; Short M; Taylor M; Su S; Hirsch V; Johnson P; Shaw G; Hahn B; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas; African green monkeys harbor a lentivirus related to human AIDS viruses in genomic organization, their gene products, cellular tropism and cytopathology for CD4 bearing T lymphocytes. In contrast to HIV-1, the simian viruses (SIVagm) vary to a much greater degree in nucleotide sequence than HIV-1 strains. African gree
Type-specific neutralizing determinant in the SIVmac transmembrane protein.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:25 (abstract no. 9). Unique Identifier : AIDSLINE PRIM8/900009; Kodama T; Burns D; Silva D; di Marzo Veronese F; Desrosiers R; Department of Microbiology and Molecular Genetics, New England; Regional Primate Research Center, Harvard Medical School,; Southborough, MA, USA; Monoclonal antibody SF8/5E11, which recognizes the transmembrane protein (TMP) of simian immunodeficiency virus of macaque monkeys (SIVmac), displayed strict type-specificity. It reacted with cloned and uncloned SIVmac251 but not with cloned SIVmac142 and SIVmac239 on immunoblots. This monoclonal antibody neutralized
Receptor binding mediates activation of fusogenic events in viral penetration.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:26 (abstract no. 10). Unique Identifier : AIDSLINE PRIM8/900010; Allan JS; Short M; Bradford D; Whitehead E; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas; Recent studies have suggested that events involved in viral entry are initiated by receptor binding to the viral membrane. Based on our previous studies we proposed that CD4, either as soluble form or as a cell membrane constituent, activates the viral membrane by inducing conformational changes leading to viral fusio
Influence of cytokines on SIV replication in macrophages.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:27 (abstract no. 11). Unique Identifier : AIDSLINE PRIM8/900011; Horvath CJ; Walsh DG; Daniel MD; Sehgal PK; Desrosiers RC; Ringler DJ; Harvard Medical School, New England Regional Primate Research; Center, 1 Pine Hill Dr., Southborough, Mass., USA; We and others have previously shown that tissue macrophages represent important targets for SIV replication in vivo and in vitro. Control of lentiviral replication within macrophages is likely influenced by a variety of viral and cellular factors; of these cellular factors, cytokines have received significant attentio
Viral and cellular gene expression in CD4 positive lymphoid cells infected by the simian immunodeficiency virus, SIV/Mne: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:28 (abstract no. 12). Unique Identifier : AIDSLINE PRIM8/900012; Agy MB; Foy K; Gale MJ; Clark EA; Katze MG; Department of Microbiology and Regional Primate Research Center,; University of Washington, Seattle WA 98195; Our laboratory has undertaken an analysis of cellular and viral gene expression in human CD4 positive cells infected by the human and simian immunodeficiency viruses. The purpose of our current study was two-fold: (i) To examine the effects of SIV/Mne infection on cellular gene expression in susceptible human CD4 posi
Induction of infection of rhesus monkeys with topical instillation of SIV/B670 into the conjunctival sac.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:29 (abstract no. 13). Unique Identifier : AIDSLINE PRIM8/900013; Conway MD; Murphey-Corb M; Davison-Fairburn B; Martin L; Delta Regional Primate Research Center, Tulane University,; Covington, LA; Laboratory accidents in which HIV is accidently instilled into the conjunctival cul de sac provide a means by which the retrovirus comes in contact with a mucous membrane and may provide a route for systemic infection. We tested the ability of SIV, a lentivirus immunologically and antigenically related to HIV, to indu
Early encephalopathy in the SIV model.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:30 (abstract no. 14). Unique Identifier : AIDSLINE PRIM8/900014; Hurtrel B; Chakrabarti L; Hurtrel M; Maire MA; Dormont D; Montagnier L; Institut Pasteur, Paris, France; The development of non human primate models is crucial for the understanding of the first stages of HIV-CNS infection. Rhesus macaques were inoculated by intravenous (IV) or intracerebral (IC) routes with SIV mac 251 and regularly sacrificed (7, 15 days,1,2 and 3 months). CNS was studied by light microscopy, immunohis
Characterization of simian immunodeficiency virus fusion peptide.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:31 (abstract no. 15). Unique Identifier : AIDSLINE PRIM8/900015; Lambrecht B; Brasseur R; Bex F; Burny A; Horth M; Department of Molecular Biology, University of Brussels, 67 rue; des Chevaux, 1640 Rhode-St-Gen/gese, Belgium; The aim of our work is the study of the fusogenic domain of SIV glycoproteins: this highly hydrophobic region is located just after the cleavage site at the amino-terminus of the transmembrane glycoprotein. The tridimensional conformation in a lipid bilayer, of fusion peptides of 30 syncytia-inducing viruses including
HIV-2 infection in macaque species.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:32 (abstract no. 16). Unique Identifier : AIDSLINE PRIM8/900016; Morton WR; McClure J; Schmidt A; Kuller L; Gale M; Hu SL; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; Human immunodeficiency virus type 2 (HIV-2) has a high degree of homology to the simian immunodeficiency virus (SIV), which has been shown to cause AIDS in several species of nonhuman primates. Use of HIV-2-infected macaques as an animal model of AIDS has several advantages, and may well limit the need for the use of
Maternal-fetal transmission of SIV in macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:33 (abstract no. 17). Unique Identifier : AIDSLINE PRIM8/900017; Ochs HD; Morton WR; Zhu Q; Kuller LD; Wu YP; Thouless ME; Benveniste RE; Department of Pediatrics, University of Washington, Seattle, WA; Infants and children have become the fastest growing HIV-infected segment in the U.S. and world-wide, reflecting an increase in HIV-infected women of childbearing age. To study the mechanisms of maternal-fetal transmission and to test strategies to prevent infection of the fetus, we have developed a model of maternal-
Maternal transmission of SIVsmm in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:34 (abstract no. 18). Unique Identifier : AIDSLINE PRIM8/900018; McClure H; Anderson DC; Fultz PN; Ansari AA; Jehuda-Cohen T; Villinger F; Klumpp SA; Switzer W; Lockwood E; Brodie A; Yerkes Primate Research Center and Department of Pathology, Emory; University, Altanta, GA; Studies to evaluate the perinatal transmission of SIVsmm were initiated by infecting 15 timed pregnant rhesus monkeys with SIVsmm during various stages of gestation and monitoring the offspring for evidence of virus infection. Three groups of five animals were experimentally infected with SIVsmm during early (day 28-3
Interactions of rhesus cytomegalovirus (CMV) and simian immunodeficiency virus (SIV) in co-infected macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:35 (abstract no. 19). Unique Identifier : AIDSLINE PRIM8/900019; Alcendor DJ; Vogel PJ; Martfeld DJ; Barry PA; Dandekar S; Luciw PA; Departments of Medical Pathology and Internal Medicine,; University of California, Davis, CA 95616; Infection of rhesus macaques with rhesus cytomegalovirus ( CMV ) is widespread at the California Primate Research Center (CPRC). As with infection with CMV in the majority of healthy humans, infection of healthy macaques with r
Serial pathogenesis study of SIV brain infection.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:36 (abstract no. 20). Unique Identifier : AIDSLINE PRIM8/900020; Sharer LR; Michaels J; Murphey-Corb M; HU FS; Kuebler DJ; Baskin GB; New Jersey Medical School, Newark, NJ; In order to determine the early sequence of events in the central nervous system (CNS) following experimental SIV infection, in situ hybridization (ISH) using a radiolabeled RNA probe of SIV/Delta D915 was performed on snap frozen brain tissue from 24 juvenile rhesus macaques. Twenty animals (nursery reared and
Transmission of SIV infection by transfusion of seronegative blood in nonhuman primates.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:37 (abstract no. 21). Unique Identifier : AIDSLINE PRIM8/900021; Jehuda-Cohen T; Villinger F; McClure HM; Lockwood E; Powell JD; Ansari AA; Department of Pathology, Emory University School of Medicine,; Atlanta, GA 30322; Our laboratory has provided several lines of evidence that document the existence of SIVsmm and HIV-1 infected vet seronegative nonhuman primates and humans, respectively. These findings prompted us to determine whether blood and blood products from such seronegative monkeys could transmit infection and disease in nai
Numbers of functional bone marrow colony-forming cells decrease late in SIV infection of rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:38 (abstract no. 22). Unique Identifier : AIDSLINE PRIM8/900022; Beltz LA; Narayan O; Adams RJ; Zink MC; Donnenberg AD; The Johns Hopkins University, School of Medicine, Baltimore, MD; We have used a long term bone marrow (BM) culture system to estimate progenitor frequency by limiting dilution analysis (LDA) in 7 Rhesus macaques studied before and after infection with SIV251. BM cells were first separated on a Ficoll/Hypaque gradient, then depleted of T lymphocytes plus early and mature monocyte/ma
Autoimmune hemolytic anemia in SIVsmm infected macaca mulatta.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:39 (abstract no. 23). Unique Identifier : AIDSLINE PRIM8/900023; Hillyer CD; Brodie AR; Ansari AA; Anderson DC; McClure HM; Department of Pathology, School of Medicine, Emory University,; Atlanta, Georgia; Rhesus macaques (n=15) were infected with 10(4) TCID SIVsmm9 (documented by serologic conversion and reverse transcriptase activity). Blood counts (Sysmex CC-180) were obtained and differential counts were determined on blood smears stained with Wright-Giemsa. Direct antiglobulin tests (DAT) were done using mouse anti
Simian immunodeficiency virus infection of macaques: end-stage disease is characterized by widespread distribution of proviral DNA in tissues.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:40 (abstract no. 24). Unique Identifier : AIDSLINE PRIM8/900024; Hirsch VM; Zack PM; Vogel AP; Johnson PR; Department of Microbiology, Georgetown University and Laboratory; of Infectious Diseases, NIAID, NIH, Rockville, Md; A key to the development of new therapeutic modalities and vaccine strategies for AIDS is a more thorough understanding of the natural history and pathogenesis of immunosuppressive lentiviral infections in animal model systems. The most relevant model for AIDS currently available is simian immunodeficiency virus (SIV)
Soluble CD4 and the treatment of AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:41 (abstract no. 25). Unique Identifier : AIDSLINE PRIM8/900025; Letvin NL; Watanabe M; Harvard Medical School, New England Regional Primate Research; Center, One Pine Hill Drive, Southborough, MA 01772; Since the CD4 molecule is a high affinity cell surface receptor for the human immunodeficiency virus (HIV), it has been suggested that a soluble truncated form of CD4 may compete with cell surface CD4 for HIV binding and thus be of use in the therapy of AIDS. We have utilized the simian immunodeficiency virus of macaq
A CD8+, MHC class II+ lymphocyte lacking CTL function appears in rhesus monkey lymph nodes early after SIVmac infection.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:42 (abstract no. 26). Unique Identifier : AIDSLINE PRIM8/900026; Reimann KA; Snyder GB; Chalifoux LV; Miller MD; Letvin NL; Harvard Medical School, New England Regional Primate Research; Center, Southborough, MA 01772; An alteration in lymphocyte subset distribution in the peripheral blood is one of the earliest manifestations of HIV infection in humans. We have characterized the coincident changes in the secondary lymphoid organs early after virus infection utilizing the SIVmac/rhesus monkey model. In a prospective study of 4 monke
CD8+ T-cell-induced inhibition of retroviral replication is mediated at the transcriptional level.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:43 (abstract no. 27). Unique Identifier : AIDSLINE PRIM8/900027; Powell JD; Bednarik DP; Folks TM; Jehuda-Cohen T; Villinger F; McClure HM; Ansari AA; Department of Pathology, Emory University School of Medicine,; Atlanta, GA 30322; There is increasing evidence that CD8+ T cells play a crucial role in controlling HIV infection in vivo. Via et al. (Immunology Today, 11(7):250) propose that the clinical transition from the asymptomatic to disease state may be secondary to the quantitative loss of such CD8+ cells. Previously, our laboratory has show
Phenotypic and functional alterations in primate lymphoid organs following SIV and HIV infection.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:44 (abstract no. 28). Unique Identifier : AIDSLINE PRIM8/900028; Rosenberg YJ; White BD; Lewis M; Mosca J; Papermaster SF; Kersey K; Eddy GA; Burke DS; Henry M. Jackson Foundation, Rockville, MD; The aim of the present study is to examine the susceptibility of lymphoid organs to infection with HIV and SIV and to compare alterations in the phenotypic and functional properties of those T cell subsets which may be differentially affected by infection. The anti-CD45RA MAb ALB11 delineates human and monkey T cells
Antibody dependent enhancement and neutralization pattern of sera of SIV infected or HIV2 vaccinated rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:45 (abstract no. 29). Unique Identifier : AIDSLINE PRIM8/900029; LeGrand R; Vogt G; Dormont D; SSA/DPTE/DSV/CEA,BP 6, 92265 Fontenay aux Roses, France; OBJECTIVE: To evaluate the antibody dependent enhancement (ADE) and the neutralizing titer of sera of rhesus monkeys infected with SIVMAC251 or immunized with Vaccinia-HIV2ROD recombinant viruses and purified HIV2 proteins. METHODS: -Animals: 1) Six Maccaca mulata were infected with 1000 TCID50 of SIVMAC251. 2) Five m
Virus neutralizing antibodies in SIV and type D vaccinated macaques, and in animals infected with SIV/Mne.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:46 (abstract no. 30). Unique Identifier : AIDSLINE PRIM8/900030; Benveniste R; Mann D; Kuller L; Morton W; Hu SL; Eddy G; Shafferman A; Lewis M; National Cancer Institute and Southern Research Institute,; Frederick, MD; An EBV transformed human cell line (AA-2) had previously been shown by us to be very sensitive to the replication of SIV s isolated from mangabeys, African green monkeys and macaques, as well as to HIV-1 and HIV-2. Multiple single-cell clones have now been obtained that differ markedly in their ability to replicate th
SIV external envelope glycoprotein is the target of neutralizing antibodies.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:47 (abstract no. 31). Unique Identifier : AIDSLINE PRIM8/900031; Collignon C; Kent K; Desrosiers R; DeWilde M; Bruck C; Thiriart C; To investigate the antigenic and biological properties of SIV envelope glycoproteins, antibodies to SIV envelope antigen (molecular clone BK28) were obtained: i) a panel of monoclonal antibodies was isolated from a mouse immunized with SIV GP150 expressed in mammalian cells and ii) polyclonal antibodies were raised to
Cloning and expression of primate CD4 molecules: diversity of the cellular receptor for SIV/HIV.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:48 (abstract no. 32). Unique Identifier : AIDSLINE PRIM8/900032; Fomsgaard A; Hirsch VM; McClure HM; Novembre FJ; Johnson PR; Department of Microbiology, Georgetown University and Laboratory; of Infectious Diseases, NIAID/NIH, Rockville, Md.; The pathogenic properties of non-human primate lentiviruses (SIV) are varied, and may depend in part on host-specific factors. Biologic disparity among SIVs also extends to infection of cells in culture, where significant differences in cellular tropisms and cytopathic effects are observed. One potential determinant o
SIV vaccine protection of rhesus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:49 (abstract no. 33). Unique Identifier : AIDSLINE PRIM8/900033; Carlson JR; McGraw TP; Keddie E; Yee JL; Rosenthal A; Langlois AJ; Dickover R; Donovan R; Luciw PA; Jennings MB; et al; Departments of Pathology and Internal Medicine, School of; Medicine, California Primate Research Center, University of; California, Davis; Rhesus macaques (M.mulatta) immunized with an inactivated whole SIVmac vaccine and muramyl dipeptide (MDP) incomplete Freund s adjuvant (IFA) or aqueous suspension were challenged intravenously with 10 animal infectious doses (ID) (0.1 TCID/50) of cell free SIVmac. Whereas virus was readily recovered from the PBLs of
Neutralizing antibodies generated in macaques immunized with live recombinant vaccinia virus boosted with recombinant-made SIVmne gp160.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:50 (abstract no. 34). Unique Identifier : AIDSLINE PRIM8/900034; Hu SL; Abrams K; Barber G; Moran P; Zarling JM; Kuller L; Morton WR; Benveniste RE; Oncogen, Seattle WA; We showed previously that immunization with HIV-1 envelope glycoprotein gp160 resulted in significantly enhanced immune responses and anti-HIV-1 neutralizing antibodies in mice and in humans primed with live recombinant vaccinia virus expressing the same antigen. In the present work, we aimed to determine, first, whet
Vaccination against primate lentiviruses in cynomolgus monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:51 (abstract no. 35). Unique Identifier : AIDSLINE PRIM8/900035; Putkonen P; Thorstensson R; Biberfeld G; Department of Immunology, National Bacteriological Lab,; Stockholm, Sweden; The related SIVsm and HIV-2 can infect cynomolgus monkeys, but only SIV causes an immunosuppressive disease. At NBL two vaccine studies have been done. In the first experiment, three macaques were preinfected and immunized with live HIV-2 (SBL-K135) and 168 days later challenged with 10-100 animal ID50 of SIV to study
Protection of rhesus macaques from infection with SIVMAC using a formalin inactivated whole virus preparation.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:52 (abstract no. 36). Unique Identifier : AIDSLINE PRIM8/900036; Cranage MP; Cook N; Thompson A; Greenaway PJ; Baskerville A; Public Health Laboratory Service Centre for Applied Microbiology; and Research, Porton Down, Salisbury SP4 OJG, United Kingdom; Eight rhesus macaques were inoculated with formalin inactivated SIVMAC251 (32H isolate) prepared from cell free supernatant of infected C8166 cells by gel filtration chromatography. Each animal received a total of four intramuscular inoculations of 500 mug administered in threonyl muramyl dipeptide adjuvant (kindly pr
A vaccine of conserved SIV envelope peptides protects macaques against SIV challenge.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:53 (abstract no. 37). Unique Identifier : AIDSLINE PRIM8/900037; Eddy GA; Shafferman A; Jahrling PB; Benveniste RE; Lewis MG; Phipps TJ; Eden-McCutchan F; Sadoff J; Burke DS; Jackson Foundation Research Lab, Rockville, MD; Rhesus monkeys were immunized with a cocktail of four recombinant-origin fusion proteins of beta-galactosidase (beta-gal) and simian immunodeficiency virus (SIV) envelope peptides, SIV-88, SIV-500, SIV-582 and SIV-647. The peptides are 10 to 21 residue segments of conserved, hydrophilic regions of SIV gp120 and transm
Protective responses against HIV-1 elicited by immunization of chimpanzees.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:54 (abstract no. 38). Unique Identifier : AIDSLINE PRIM8/900038; Fultz PN; Pinter A; Nara P; Muchmore E; Kaczorek M; Girard M; University of Alabama at Birmingham; Candidate HIV-1 vaccines, consisting of either whole inactivated virus, purified recombinant antigens (gp160env, p18gag, p27nef p23vif) or peptides conjugated to KLH, and corresponding to the V3 neutralizing epitope, were generated with the HIV-1 (LAV(BRU) strain) and used to immunize chimpanzees. Immunization resulte
Immunization of healthy SIV-infected macaques with a formalin inactivated whole virus vaccine has no apparent effect on disease progression and survival.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:55 (abstract no. 39). Unique Identifier : AIDSLINE PRIM8/900039; Murphey-Corb M; Davison-Fairburn B; Ohkawa S; Martin L; Baskin G; Delta Regional Primate Research Center, Tulane University,; Covington, LA; The association of the decline in HIV-specific antibody titers, specifically with respect to core determinants, to disease progression in infected humans, coupled with the relatively long latency between virus infection and the onset of disease, promotes the concept that immunization of healthy seropositive individual
Effects of 28 day treatment with azidothymidine (AZT) initiated 1 to 72 hours after infection with simian immunodeficiency virus (SIV).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:56 (abstract no. 40). Unique Identifier : AIDSLINE PRIM8/900040; Martin LN; Murphey-Corb M; Soike KF; Davison-Fairburn B; Delta Regional Primate Research Center, Tulane University,; Covington, LA, U.S.A.; We have studied the effects of AZT treatment initiated 1, 8, 24 or 72 hr. postinfection (PI) with 10 ID-50 SIV/Delta B670 using 5 monkeys per group. After initiation, the animals were treated every 6 hr. with 25 mg/kg (100 mg/kg/day) for 28 days. AZT
Inhibition of SIVmac by zidovudine (AZT) and 2', 3' Dideoxy-2', 3'-didehydrothymidine (D4T) in combination with the membrane-active drug dipyridamole (DPM).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:57 (abstract no. 41). Unique Identifier : AIDSLINE PRIM8/900041; Fridland A; Robbins B; Connelly MC; Gould R; Marx PA; St. Jude Children's Research Hospital, 332 North Lauderdale, Box; 318, Memphis, TN 38101; D4T and AZT are two major drugs used in the treatment of HIV infections. However the anti-viral activity of these drugs is limited by their ability to form the 5 triphosphate derivatives which are believed to act as chain terminators of reverse trans
The immunobiological properties of a recombinant SRV-1 envelope protein and a neutralizing monoclonal antibody directed against it.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:58 (abstract no. 42). Unique Identifier : AIDSLINE PRIM8/900042; Werner LL; Torres JV; Kwang HS; Leung CY; Benjamini E; Department of Clinical Pathology, School of Veterinary Medicine,; and Department of Medical Microbiology and Immunology, School of; Medicine, University of California, Davis, CA; A synthetic peptide representing residues 142-167 contains a unique envelope region of the Simian (Type D) Retrovirus serotype 1 (SRV-1) and constitutes an antigenic site for the binding of murine and rhesus neutralizing antibodies to the SRV-1 strain. A murine monoclonal antibody, C11B8, produced by immunizing with a
The induction of anti-simian retrovirus-1 (SRV-1) neutralizing antibodies by synthetic peptides and studies on the cell receptor to the virus.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:59 (abstract no. 43). Unique Identifier : AIDSLINE PRIM8/900043; Torres J; Werner L; Malley A; Benjamini E; Department of Medical Microbiology and Immunology, School of; Medicine, University of California, Davis; We have recently demonstrated that rhesus monkey antibodies which neutralize SRV-1 are directed to an area encompassing residues 147-162 of the viral envelope protein. Although immunization of mice with the synthetic peptide-KLH conjugate induced antibodies which bind with the peptide, with the viral protein and with
Characterization of T and B cell epitopes of a simian retrovirus (SRV-2) envelope protein.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:60 (abstract no. 44). Unique Identifier : AIDSLINE PRIM8/900044; Malley A; Werner L; Benjamini E; Leung CY; Torres J; Pangares N; Shiigi S; Axthelm M; Oregon Regional Primate Research Center, Beaverton OR; Previously, we reported that the insoluble SRV-2 envelope peptide 92-106 represented the epitope involved in the formation of SRV-2 neutralizing antibodies. Soluble SRV-2 peptide 92-99 and peptide 100-106 were prepared and used to further define the epitope responsible for the induction of SRV-2 neutralizing antibody.
Further studies on SRV-2 positive retroperitoneal fibromatosis (RF) derived cell lines from macaques with simian AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:61 (abstract no. 45). Unique Identifier : AIDSLINE PRIM8/900045; Roodman ST; Tsai CC; Tsai CC; Woon MD; St. Louis University School of Medicine, St. Louis, MO; Several cell lines were derived from Kaposi s Sarcoma (KS) like-RF lesions obtained from pigtailed macaques (Macaca nemestrina) infected with SRV-2. SRV-2 antigens were detected on these cell lines by flow cytometric (FCM) and whole cell ELISA using either SRV-2 infected monkey serum or monoclonal anti gp44 antibodies
Genomic organization, transcriptional regulation, and transactivation of simian foamy virus type 1: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:62 (abstract no. 46). Unique Identifier : AIDSLINE PRIM8/900046; Mergia A; Shaw KE; Pratt-Lowe E; Barry PA; Luciw PA; Department of Medical Pathology, University of California, Davis,; CA 95616; The three subfamilies of retroviruses are oncoviruses, lentiviruses, and spumaviruses. The latter, also designated foamy or syncytium-forming viruses, are found in a variety of species and are highly cytopathic in tissue culture systems. In the animal, spumaviruses appear to establish latency; a clear connection with
Functional analysis of the major homology region (MHR) of M-PMV capsid protein using saturation mutagenesis: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:63 (abstract no. 47). Unique Identifier : AIDSLINE PRIM8/900047; de Castillia CS; Hunter E; University of Alabama at Birmingham, Department of Microbiology,; Birmingham, AL. 35294; The major capsid protein (CA) of avian and mammalian retroviruses is thought to play a critical role in: 1) capsid assembly and release from the cell, 2) maturation of the released virus particles, 3) uncoating of the viral genome early after infection, 4) proviral DNA transport to the nucleus and integration in the c
Baboon: an animal model for human retroviral diseases.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:64 (abstract no. 48). Unique Identifier : AIDSLINE PRIM8/900048; Mone J; Hubbard G; Whitehead E; Allan JS; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas; The baboon (Papio sp.) is an old-world primate which is a natural host for simian T-cell leukemia virus type 1 (STLV-1), a virus very similar to human T-cell leukemia virus type 1 (HTLV-1), the etiologic agent of adult T-cell leukemia/lymphoma. Furthermore, STLV-1 has been associated with malignant lymphomas in baboon
Biological characterization of HIV-IFN-gamma fusion proteins expressed in vaccinia virus: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:65 (abstract no. 49). Unique Identifier : AIDSLINE PRIM8/900049; Yilma T; Gibson HL; Lee Ng CT; Barr PJ; Giavedoni L; Lab of Mol. Bio. Trop. Dis., School of Veterinary Medicine,; University of California, Davis, CA; We have evaluated the adjuvant effects of interferon gamma (IFN-gamma) on heterologous antigens expressed in vaccinia virus recombinants, and also determined the attenuating effects of the lymphokine on vaccinia virus virulence in nude (nu/nu) mice. Antigens expressed included murine IFN-gamma (vMuIFN-g), human IFN-ga
A non-hazardous HIV-1 envelope glycoprotein expression vector system for development of anti-HIV therapy.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:66 (abstract no. 50). Unique Identifier : AIDSLINE PRIM8/900050; Roberts SJ; Dubay JW; Hunter E; Department of Microbiology, University of Alabama at Birmingham,; Birmingham, Alabama; A useful SV40 late promoter based expression vector, pSRH, has been developed and used successfully for the high-level expression of the HIV-1 envelope glycoprotein. The vector contains the SV40 origin of replication, the early genes (T and t) and the early and late promoters. Polyadenylation sequences are from the 3
High titers of HIV-1 reverse transcriptase inhibitory antibodies in the sera of African green monkeys vaccinated with a prototype HIV-1 vaccine: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:67 (abstract no. 51). Unique Identifier : AIDSLINE PRIM8/900051; Bestwick RK; Yoshihara P; Martin L; Leslie G; Hodges W; Benelli-LeMoine M; Ferro A; Epitope, Inc. 15425 SW Koll Parkway, Beaverton, OR 97006; Other than antibodies against the gp41 transmembrane glycoprotein, antibodies against reverse transcriptase are the most prevalent in HIV-infected individuals. The level of antibodies that inhibit reverse transcriptase decrease in patients that progress to AIDS and the presence or absence of these inhibitory antibodie
Vaccination of cynomolgus monkeys against simian immunodeficiency virus: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:68 (abstract no. 52). Unique Identifier : AIDSLINE PRIM8/900052; Stott EJ; Cranage MP; Chan WL; Mills K; Page M; Taffs F; Greenaway P; Kitchin P; National Institute for Biological Standards and Control, Blanche; Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK.; Cynomolgus monkeys were vaccinated with either glutaraldehyde-fixed SIV-infected cells using Quil A as adjuvant or with formalin-fixed whole SIV using tMDP as adjuvant. They were subsequently challenged intravenously with 10 fifty per cent monkey infectious doses (MID50) of 11/88 pool of SIVmac251. The vaccinated anim
Biochemical and antigenic properties of SIVMne gp160 expressed in insect cells by a baculovirus vector.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:69 (abstract no. 53). Unique Identifier : AIDSLINE PRIM8/900053; Barber G; Gale M; Clark EA; Hu SL; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; A cDNA encoding the full-length precursor envelope glycoprotein of SIVMne has been inserted into the genome of Autographica californica nuclear polyhedrosis virus (AcNPV) under control of the viral polyhedrin gene promotor. Infection of cultured Spodoptera frugiperda cells with recombinant virus resulted in the synthe
Development of potential prophylactic and immunotherapeutic agents against SIV: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:70 (abstract no. 54). Unique Identifier : AIDSLINE PRIM8/900054; Rud EW; Yon JR; Cranage MP; Francis MJ; Larder BA; Clarke BE; Wellcome Biotechnology Ltd., Dept of Virology, Beckenham, Kent,; U.K.; Simian Immunodeficiency Virus (SIV) is, in many respects, the best animal model for the study of human AIDS. In order to develop candidate subunit vaccines against SIV, we started by cloning the vaccine challenge strain, SIVmac32H, used by the British M.R.C. AIDS Directed Programme. We made three molecular clones, dif
Mutations in the cysteine array of the nucleic acid binding protein of SIV/MNE: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:71 (abstract no. 55). Unique Identifier : AIDSLINE PRIM8/900055; Powell PJ; Henderson LE; Heidecker G; Benveniste RE; Arthur LO; AIDS Vaccine Program, Program Resources Inc., Frederick MD 21702; Recent studies have shown that vaccines composed of whole inactivated preparations of simian immunodeficiency virus (SIV) gave protection against SIV challenges. These preparations should be considered as candidates for vaccine and immunotherapy for AIDS. Ideally the vaccine should contain all the viral proteins in a
The efficient expression of simian immunodeficiency virus envelope protein by a recombinant human adenovirus host-range mutant: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:72 (abstract no. 56). Unique Identifier : AIDSLINE PRIM8/900056; Sheau-Mei C; Ronchetti-Blume M; Hum W; Politis-Virk K; Mizutani S; Davis A; Lee S; Hung P; Hirsch V; Johnson P; Wyeth-Ayerst Research, Radnor, PA; SIV infection of macaques is a valuable animal model for AIDS vaccine studies. Our research focus is the development of Ad-HIV vaccine by using the Ad-SIV recombinant virus infection of macaques as a model system. Recombinant human adenoviruses (Ads) that replicate in the intestinal tract offer a novel, yet practical,
Immunological properties of recombinant SIV gp130 expressed in genetically engineered mammalian cells: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:73 (abstract no. 57). Unique Identifier : AIDSLINE PRIM8/900057; Planelles V; Haigwood N; Scandella C; Mann K; Shuster J; Marthas M; Marx P; Gardner M; Luciw P; University of California, Davis; The simian immunodeficiency virus (SIV) envelope glycoprotein consists of two major domains, the extracellular domain (gp130) and the transmembrane domain (gp30). Gp130 is (i) the major viral element responsible for attachment to the cell receptor, CD4 and (ii) the main target against which anti-viral immune responses
The effect of recombinant human alpha interferon on SIV infection in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:74 (abstract no. 58). Unique Identifier : AIDSLINE PRIM8/900058; Niphuis H; Schellekens H; Slachmuylders JF; Hochkeppel HK; Heeney JL; Dept. of Chronic and Infectious Diseases, ITRI-TNO, Rijswijk, The; Netherlands; The activities of recombinant hybrid human interferon alpha-BDBB (HuIFNa-BDBB) and recombinant human interferon alpha-2 (HuIFNa-2) were compared on their ability to inhibit infection with SIVDeltaB670 in rhesus macaques. Four groups of two animals were treated with different doses of HuIFNa (5.10(6) or 1.10(6)units/da
Applications of the SIV/rhesus model for the sexual transmission of HIV: comparison of various contraceptive formulations containing nonoxynol-9 on the genital transmission of SIV in rhesus macaques and the relationship between viremia humoral immune response and the shedding of SIV in semen.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:75 (abstract no. 59). Unique Identifier : AIDSLINE PRIM8/900059; Miller CJ; Alexander NJ; Gettie A; Hendrickx AG; Marx PA; California Primate Research Center and Department of Medical; Pathology, University of California, Davis, CA; Recent in-vitro studies in which HIV was inactivated by low concentrations (0.05% v/v) of nonoxynol-9 (N-9) suggest that the heterosexual transmission of human immunodeficiency virus (HIV) can be prevented by the use of spermicides that contain N-9. However there have been no studies to assess the anti-viral activity
Use of SIV-infected monkeys in the evaluation of drugs against HIV/AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:76 (abstract no. 60). Unique Identifier : AIDSLINE PRIM8/900060; Bottiger D; Ljungdahl-Stahle E; Norrby E; Putkonen P; Wahren B; Oberg B; Department of Virology, Karolinska Institute, Stockholm, Sweden; Many new compounds are being synthesized with the aim of treating AIDS. Several of these compounds show good inhibitory effects against HIV in cell cultures and this requires a further selection in a relevant animal model in order to select the few compounds possible to evaluate in patients. Simian immunodeficiency vi
Prophylactic effect of HOE/BAY 946, a new anti-viral agent, on SIV and simian AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:77 (abstract no. 61). Unique Identifier : AIDSLINE PRIM8/900061; Cheung AT; Lerche NW; Schrinner EJ; Schlumberger HD; Young LJ; Yee JL; Department of Medical Pathology and California Primate Research; Center, University of California, Davis, California, USA; HOE/BAY 946, a disodium salt of xylanpoly-hydrogen sulphate, shows significant in vitro inhibitory activities against the reverse transcriptases of retroviruses, including SIV, HIV, AMV, and FLV. The pharmacokinetic profile of this compound is known and it is currently used for other indications with no significant ad
Bone marrow progenitor cells in SIV infected rhesus monkeys remain uninfected despite hematologic abnormalities in AIDS.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:78 (abstract no. 62). Unique Identifier : AIDSLINE PRIM8/900062; Slachmuylders JF; Wagemaker G; Visser TP; Dubbes R; Niphuis H; Bentvelzen PA; Heeney JL; Institute of Applied Radiobiology and Immunology TNO, P.O. Box; 5815, 2280 HV Rijswijk, The Netherlands; Macaca mulatta experimentally infected with SIVDeltaB670 or SIVmac32H develop a spectrum of hematological abnormalities including thrombocytopenia, anemia and generalized marrow hypoplasia in advanced disease. The underlying nature of these lesions in HIV infected patients or SIV infected Rhesus monkeys with AIDS is u
HLA-DR antigen in neuroendothelium is detected during SIVSM encephalitis.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:79 (abstract no. 63). Unique Identifier : AIDSLINE PRIM8/900063; Beilke MA; Gravell M; Jordan EK; Riding In D; Hamilton R; Nusbaum W; Stone G; Laboratory of Central Nervous System Studies, NINDS, NIH,; Bethesda, MD; Major histocompatibility complex class 2 (MHC II, HLA-DR) antigens generally are not expressed in primate brain. Cerebral microvessels were isolated by dextran gradient centrifugation from two animals with SIVsm encephalitis, from one infected animal without CNS involvement and from two uninfected animals. Neuroendoth
Pathogenesis of simian immunodeficiency virus infection in the central nervous system of rhesus monkeys: cellular localization of virus.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:80 (abstract no. 64). Unique Identifier : AIDSLINE PRIM8/900064; Lackner AA; Smith MO; Munn RJ; Martfeld DJ; Marx PA; Dandekar S; California Primate Research Center and Department of Internal; Medicine, University of California, Davis, California; Simian immunodeficiency virus (SIV), like the human immunodeficiency virus (HIV), is a lentivirus that is both immunosuppressive and neurovirulent. Rhesus macaques (Macaca mulatta) inoculated with SIV often develop a giant cell encephalitis similar to that seen in humans infected with HIV. We examined SIV expression b
Ultrastructural pathology of the acutely lethal primate lentivirus SIVSM-PBj, in pigtailed and cynomolgus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:81 (abstract no. 65). Unique Identifier : AIDSLINE PRIM8/900065; Brown CR; Lewis MG; Jahrling PB; Elkins WR; Zack PM; US Army Medical Research Institute of Infectious Diseases, Fort; Detrick, MD 21702; SIVSM-PBj uniformly produces acutely fatal disease associated with severe metabolic acidosis in pigtailed and cynomolgus macaques. All lymphoid organs and gut-associated lymphoid tissue (GALT) show similar histologic changes including hyperplasia of T-cell areas containing up to 50% lymphoblasts, atrophy of B-cell are
Pathogenesis of enteropathy in SIV-infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:82 (abstract no. 66). Unique Identifier : AIDSLINE PRIM8/900066; Dandekar S; Heise C; Martfeld D; Marx P; Miller C; Halsted C; Department of Internal Medicine, University of California, Davis,; CA 95616; Diarrhea with intestinal malabsorption can occur early in the course of HIV infection, often in the absence of opportunistic enteric infections. We have used SIV-infected rhesus macaques as an animal model to determine the pathogenic mechanisms of enteropathy in HIV infection. The major objectives were (1) to evaluate
SIV/Delta-induced arthritis in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:83 (abstract no. 67). Unique Identifier : AIDSLINE PRIM8/900067; Roberts ED; Martin LN; The joint tissues from thirty-six rhesus infected with SIV/Delta were graded into 4 groups and then correlated with the following systemic immunologic parameters: numbers of CD4+, CD4+ plus CD29+, CD8+, CD20+ cells and SIV/Delta antigenemia. Grade I (N=16) Consisted of normal synovial membrane. Grade II (N=8) Consiste
Role of rhesus cytomegalovirus (CMV) and simian immunodeficiency virus (SIV) in SIV-associated pneumonia.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:84 (abstract no. 68). Unique Identifier : AIDSLINE PRIM8/900068; Vogel AP; Lackner AA; Alcendor DJ; Martfeld DJ; Marx PA; Dandekar S; Department of Veterinary Pathology, University of California,; Davis, CA 95616; Animals infected with SIV may be clinically normal for an extended period before developing an acquired immune deficiency syndrome. Individual variation in the responses to infection by SIV suggests the involvement of multiple cofactors in determining disease course and clinical outcome. Several in vitro studies sugge
Preliminary observations on the immunophenotyping of mononuclear cells and the detection of viral antigens using formalin-fixed, paraffin-embedded sections from SIV-infected macaque monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:85 (abstract no. 69). Unique Identifier : AIDSLINE PRIM8/900069; Ribas JL; Sesterhenn IA; McClure HM; Anderson DC; Frizzera G; Benton CS; Armed Forces Institute of Pathology, Washington, DC; The localization of specific antigens in tissues and cells by immunohistochemical methods has become a powerful tool in diagnostic and experimental pathology. Since the simian immunodeficiency virus (SIV) infects mainly CD4-bearing cells of the immune system of susceptible macaque monkeys, immunophenotyping of mononuc
Infection of macaques with SIV or HIV-2 induces a virus-specific increase of urinary neopterin.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:86 (abstract no. 70). Unique Identifier : AIDSLINE PRIM8/900070; Stahl-Hennig C; Fendrich C; Lu\uke W; Fuchs D; Reibnegger G; Wachter H; Hunsmann G; German Primate Center, Go\uttingen, FRG; Neopterin is known as a marker for the activated immune system. Increased neopterin concentrations have been observed in HIV-infected humans and also in SIV-infected macaques. To extend the latter data preinfection and postinfection urinary neopterin concentrations were measured in rhesus (Mm) and fascicularis (Mf) ma
Mapping viral determinants of SIV pathogenesis.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:87 (abstract no. 71). Unique Identifier : AIDSLINE PRIM8/900071; Marthas M; Ramos R; Lohman B; Banapour B; Marx P; Luciw P; Pedersen N; California Regional Primate Research Center, University of; California, Davis, CA 95616; Two molecular clones of simian immunodeficiency virus with different disease potential in rhesus macaques have been characterized. SIVmac-1A11 induces a transient viremia and no disease, while SIVmac-239 induces persistent viremia and a fatal AIDS-like disease in about 50% of experimentally infected macaques within on
Structure of tat-, rev-, and env- specific transcripts of a cloned simian immunodeficiency virus from rhesus macaques (SIVmac1A11).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:88 (abstract no. 72). Unique Identifier : AIDSLINE PRIM8/900072; Unger RE; Stout MW; Luciw PA; School of Medicine, Department of Medical Pathology, University; of California, Davis, CA; Simian immunodeficiency virus from rhesus macaques (SIVmac) produces an immunosuppressive disease in these animals that is similar to that caused by human immunodeficiency virus (HIV-1), the etiologic agent of acquired immunodeficiency syndrome in humans. SIVmac, like HIV-1, has a complex genetic organization characte
Identification of a highly variable region in SIV envelope in macaques inoculated with SIV/MNE clone 8.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:89 (abstract no. 73). Unique Identifier : AIDSLINE PRIM8/900073; Rudensey L; Papenhausen M; Benveniste R; Morton WR; Overbaugh J; Regional Primate Research Center, University of Washington,; Seattle, WA.; The study of retroviral pathogenesis, especially that of HIV, is complicated by the presence of multiple virus variants within an infected individual. In the case of HIV, the contribution of specific variants to the development of AIDS is unclear. Analysis of feline leukemia virus (FeLV) variants cloned directly from
Genetic variation of the SIVagm transmembrane glycoprotein in naturally and experimentally-infected non-human primates.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:90 (abstract no. 74). Unique Identifier : AIDSLINE PRIM8/900074; Fomsgaard A; Johnson PR; Dapolito G; Kitov S; London WT; Hirsch VM; Department of Microbiology, Georgetown University, Washington, DC; Premature truncation of the gp40 transmembrane glycoprotein (TMP) is commonly manifested by some SIV strains when propagated in human cells in culture. However, previous studies with SIVmac indicate that the natural form of the TMP is the full-length form predicted from the entire env open reading frame. To further in
Expression of SIV in CD4+ lymphocytes.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:91 (abstract no. 75). Unique Identifier : AIDSLINE PRIM8/900075; Park IW; Steen R; Regier D; Li Y; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA 01772.; Both simian immunodeficiency viruses (SIVs) and human immunodeficiency viruses (HIVs) belong to the lentivirus sub-family of Retrovirudae. One of the hallmarks of these primate lentiviruses is the presence of at least five or six additional open reading frames (orfs) or genes. The functions of some of the orfs are unc
Disruption of the nef gene in a cloned simian immunodeficiency virus from rhesus macaques (SIVmac1A11) does not effect macrophage tropism in vitro.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:92 (abstract no. 76). Unique Identifier : AIDSLINE PRIM8/900076; Unger R; Padrid P; Marthas M; Pratt-Lowe E; Mulvaney T; Planelles V; Banapour B; Cross C; Luciw P; University of California, Davis, CA; Substantial sequence polymorphism has been detected among nef genes from different cloned isolates of SIVmac. In some, the nef gene is intact and in others an in-frame termination codon is present within the nef gene sequence. nef has been reported to down-regulate viral replication, since in both HIV and SIV, viruses
Simian immunodeficiency virus (SIVmac) infection of monocyte-derived macrophages from rhesus macaques: an in vitro infectivity system.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:93 (abstract no. 77). Unique Identifier : AIDSLINE PRIM8/900077; Banapour B; Vogel P; Marthas M; Unger R; Luciw P; Department of Medical Pathology, School of Medicine, University; of California at Davis. Davis, California 95616; We have developed an in vitro system for infection of primary cultures of monocyte-derived macrophages from rhesus macaques with various strains of SIVmac. This system has been utilized to study cell tropism of pathogenic and nonpathogenic clones of SIVmac. Data will be presented on the extent of infection and cytopat
Granulocyte macrophage-colony stimulating factor stimulates persistently infected U1 cells to produce infectious human immunodeficiency virus (HIV).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:94 (abstract no. 78). Unique Identifier : AIDSLINE PRIM8/900078; Krugner-Higby L; Iyer N; Kucera L; Department of Comparative Medicine, Bowman Gray School of; Medicine, 300 S. Hawthorne Rd. Winston-Salem NC 27103; U1 is a clone of the promonocytic cell line U937 persistently infected with HIV. It produces little or no virus unless stimulated. U1 may be stimulated to productive infection by phorbol esters such as TPA, but the presence of these agents in tissue culture supernatants and cell lysates causes cytotoxic effects in syn
The effect of cytokines on the replication of pathogenic and nonpathogenic isolates of SIV/Delta in monocytes.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:95 (abstract no. 79). Unique Identifier : AIDSLINE PRIM8/900079; St Cyr-Coats K; Murphey-Corb M; Dept. of Biological Sciences, Mississippi State University,; Mississippi State, MS; Neurotropic lentiviruses such as Visna-Maedi, caprine arthritis-encephalitis virus, and certain isolates of human immunodeficiency virus (HIV) replicate preferentially in cells of monocyte origin, and latently infected monocytes may be responsible for the spread of the viruses to the central nervous system. One isolat
Tumor necrosis factor alpha (TNF alpha) production by alveolar macrophages (M phi) infected in vitro or in vivo with SIV.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:96 (abstract no. 80). Unique Identifier : AIDSLINE PRIM8/900080; Horvath CJ; Desrosiers RC; Sehgal PK; Ringler DJ; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA 01772; We studied the release of TNF alpha, a vital immunoregulatory cytokine, by M phi infected with SIV in vitro or collected from SIV-infected macaques. For in vitro studies, M phi were harvested by bronchoalveolar lavage of 5 normal animals and infected in flasks with SIVmacMTV (TCID50=10(4)/2.5x10(6) M phi). After 7-10
Species variation of interleukin production associated with infection with an acutely fatal simian immunodeficiency virus isolate (SIVsm/pbj).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:97 (abstract no. 81). Unique Identifier : AIDSLINE PRIM8/900081; Lewis MG; Birx DL; Zack PM; Vahey MA; Redfield RR; Burke DS; Jahrling PB; Southern Research Inst., Fort Detrick, MD 21702; SIV/sm/pbj causes acute death of susceptible macaques within 10 days of infection. The disease is associated with a rapid onset and high levels of virus production. The virus is mostly localized in the lymph nodes spleen and lower intestinal tract, with titers as high as 10(5) infections units/gram of tissue or millil
Evidence that the seronegative sooty mangabeys in our colony are infected with SIVsmm.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:98 (abstract no. 82). Unique Identifier : AIDSLINE PRIM8/900082; Vuchetich M; Jehuda-Cohen T; Villinger F; Powell JD; McClure HM; Ansari AA; Department of Pathology, Emory University School of Medicine,; Atlanta, GA 30322; Several lines of evidence have been provided by our laboratory to indicate that the seronegative sooty mangabeys in our colony are, in fact, infected with SIVsmm. The findings include (a) the synthesis and secretion of readily detectable SIV-reactive antibodies in the supernatant fluid of PBMC cultured in vitro with p
Effect of synthetic peptide homologous to retrovirus transmembrane protein sequences on calcium second messenger signalling in Macaca nemestrina lymphocytes.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:99 (abstract no. 83). Unique Identifier : AIDSLINE PRIM8/900083; Wright KA; Marr JM; Kanda P; Lafrado LJ; Center for Retrovirus Research, 1925 Coffey Rd., Columbus, Ohio; 43210; CKS-17 is a synthetic peptide homologous to a conserved, immunoactive sequence of p15E in feline leukemia virus and murine leukemia virus, and gp21 of the human T-cell leukemia virus. We assayed the effect of this synthetic peptide on the basal intracellular free calcium (IFC) concentration and transient IFC concentra
Selective preparative isolation of primary blood-brain barrier [endothelial, choroid plexus] and central nervous system supporting [astrocyte] cells from macaques: preliminary in vitro simian immunodeficiency virus [SIV] infection studies.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:100 (abstract no. 84). Unique Identifier : AIDSLINE PRIM8/900084; Brooks BR; Madden RM; Stone CM; Schultz KT; Neurology and Research Services, William S Middleton Memorial VA; Hospital, Madison, Wisconsin, Department of Neurology and Medical; Microbiology, University of Wisconsin, Madison; The mode of entry of SIV into the central nervous system [CNS] is poorly understood. In order to better define the virus-cell interactions which might occur at the blood-brain barrier in different CNS regions, we developed a serial Dextran - Percoll Step Gradient technique to selectively isolate non-neuronal cells fro
The use of rhesus heparinized or defibrinated blood as target cells in in vitro assays for simian immunodeficiency virus.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:101 (abstract no. 85). Unique Identifier : AIDSLINE PRIM8/900085; Lohman BL; Higgins J; Marthas ML; Marx PA; Pedersen NC; California Primate Research Center, Davis, CA, USA; In vitro assays for viral neutralization, virus titration, and virus isolation are critical for evaluation of animal model vaccine trials. We developed a novel method for in vitro simian immunodeficiency virus (SIV) assays for neutralization, titrations, and isolations using rhesus heparinized or defibrinated blood as
Inhibition of a reverse transcriptase assay for simian retroviruses by different types of mycoplasma.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:102 (abstract no. 86). Unique Identifier : AIDSLINE PRIM8/900086; Ohmann S; Thouless M; Kenny G; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; While screening Macaca fasicularis for type D simian retrovirus (SRV), we assayed supernatant from co-cultures of peripheral blood mononuclear cells with Raji cells for reverse transcriptase activity. We obtained negative results for co-cultures which showed formation of syncytial cells and which were virus positive b
Effects of heat shock on the infectivity of SIV in vitro.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:103 (abstract no. 87). Unique Identifier : AIDSLINE PRIM8/900087; Follis KE; Tsai CC; Hall B; Washington Regional Primate Research Center, University of; Washington, Seattle, WA 98195; Cocultivation of human AA-2 and C-8166 cell lines with simian immunodeficiency virus (SIV) is an appropriate in vitro model for studying the cytopathic effects of lentiviruses. Using this in vitro system we studied the effects of heat shock on the activation of SIV251 and SIVMne infectivity. We also examined the effec
Characterization and in vitro titration of a large, homogeneous SIVmac251 virus pool.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:104 (abstract no. 88). Unique Identifier : AIDSLINE PRIM8/900088; Harrison RE; Eichberg JW; Southwest Foundation for Biomedical Research, San Antonio, Texas,; 78228; A large, homogeneous pool of SIVmac251 was generated by establishing a chronic infection in the H9 cell line. Virus was harvested, pooled, and characterized by western blot, PCR, and host range analysis. An in vivo titration was undertaken using pooled virus in Rhesus monkeys in order to establish the infectivity and
Comparative efficacy of methods used for isolation of HIV-1 from a large cohort of infected chimpanzees.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:105 (abstract no. 89). Unique Identifier : AIDSLINE PRIM8/900089; Murthy KK; Piazza PA; Cobb EK; Tuck P; Eichberg JW; Dept. of Virology/Immunology, Southwest Foundation for Biomedical; Research, San Antonio, TX 78227.; In this study we have evaluated several methods to optimize the recovery of HIV-1 from peripheral blood mononuclear cells (PBMC) of experimentally infected chimpanzees (N=35). The methods used were (1) cocultures of chimp PBMC with PHA induced human blasts and DEAE-Dextran. (2) cocultures of PHA stimulated chimp PBMC
Cell proliferative responsiveness in SIV infected or recombinant SIV envelope immunized monkeys.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:106 (abstract no. 90). Unique Identifier : AIDSLINE PRIM8/900090; Ohkawa S; Wilson L; Murphy-Corb M; Martin L; Delta Regional Primate Research Center, Covington, LA 70433; Antigen specific blastogenic cell responsiveness was assessed in mononuclear cells(MNC) from infected or immunized monkeys. Monkeys immunized with recombinant glycoprotein140(rGP140) in Freund complete adjuvant showed good blastogenic responsiveness to rGP140 1 month after immunization without booster immunization. Bl
Definition of T cell subsets in macaques using quantitative three color flow cytometry.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:107 (abstract no. 91). Unique Identifier : AIDSLINE PRIM8/900091; Axberg I; Gale MJ; Afar B; Clark EA; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; Monitoring changes of T lymphocyte subsets in SIV and HIV-infected individuals can provide important information about immune and clinical status. Two color flow cytometry using fluorescein (FITC)-conjugated monoclonal antibodies (mAb) (GREEN) and R-phycoerythrin (PE)-conjugated mAB (ORANGE) has been used e.g., to mon
Cellular and cytokine requirements for the in vitro synthesis of SIV-reactive antibodies by polyclonal activation of PBMC from infected yet seronegative nonhuman primates.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:108 (abstract no. 92). Unique Identifier : AIDSLINE PRIM8/900092; Ansari AA; Jehuda-Cohen T; Powell JD; Villinger F; Mayne A; Lockwood E; Gordon T; McClure HM; Department of Pathology, Emory University School of Medicine,; Atlanta, GA 30322; In vitro polyclonal activation of PBMC from seronegative sooty mangabeys led to the secretion of antibodies that specifically react with SIV. This finding not only led to the subsequent observation that such seronegative mangabeys are indeed infected with SIV yet remain seronegative, but a similar study of a cohort of
Seroreactivity to Nef in three species of SIV/Mne-infected macaques at the Washington Regional Primate Research Center.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:109 (abstract no. 93). Unique Identifier : AIDSLINE PRIM8/900093; Heidecker G; Hill R; Morton W; Benveniste R; National Cancer Institute, Frederick, MD 21702; To investigate the immune response to non-structural viral proteins in SIV infected macaques we have studied the antibody response to the regulatory Nef protein. The Nef protein of SIV/Mne clone8 was expressed to high levels in the Sf9/baculovirus expression system. Only extracts from Sf9 cells infected with virus car
Monoclonal antibody mapping and strain-distribution of six epitopic regions of SIVmac(Davis).: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:110 (abstract no. 94). Unique Identifier : AIDSLINE PRIM8/900094; Higgins J; Pedersen N; Sutjipto S; Hansen D; Gettie A; Marx PA; Department of Veterinary Medicine, University of California,; Davis, CA 95616; The immunogenicity of the p27 major core antigen of Simian Immunodeficiency Virus from rhesus macaques (SIVmac) is well established in animal models. The role of this immune response is hypothesized to be important in both cell-mediated and humoral immunity. The characterization of epitopes of SIV p27 provide useful i
Monoclonal antibodies to SIV proteins.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:111 (abstract no. 95). Unique Identifier : AIDSLINE PRIM8/900095; Kent K; Stallard G; Gritz L; Cranage M; Thiriart C; Collignon L; Silvera P; Corcoran T; Stott J; National Institute for Biological Standards and Control, Potters; Bar, Herts, UK.; Eighteen MAbs to env and 16 MAbs to gag were produced and characterised. ELISA titres of ascites of the env MAbs were between 2.4 and 5.8 log10. Eleven of the env MAbs recognize gp160 and gp120 in Western blot (WB), 5 react with gp160 and gp41 and 2 MAbs did not react with denatured antigen. All env MAbs precipitated
A simple, rapid immunoassay for the detection of simian immunodeficiency virus antibodies.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:112 (abstract no. 96). Unique Identifier : AIDSLINE PRIM8/900096; Yee JL; Jennings MB; Carlson JR; Lerche NW; Department of Medical Pathology, University of California, Davis; The detection of Simian Immunodeficiency Virus (SIV) antibodies has proven to be useful for a wide variety of research studies, including estimating prevalence and directing efforts to obtain new virus isolates. However, the requirements of conventional assays for refrigeration, electricity supply, sophisticated equip
Improved detection of SIV p27 by antigen capture ELISA.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:113 (abstract no. 97). Unique Identifier : AIDSLINE PRIM8/900097; Toedter G; Stoner S; Pearlman S; Brewer K; Charie L; Cueto C; Marquez M; Hofheinz D; Coulter Immunology, Hialeah, Florida 33010; The limited sensitivity of HIV-1 p24 antigen capture ELISA systems for SIV core antigens has been documented. A murine monoclonal antibody-based antigen capture ELISA has been developed to detect SIV p27. This assay has been demonstrated to have greater sensitivity for SIV than existing antigen capture assays or rever
Differences in T cell function in rhesus and pigtailed monkeys: implications regarding susceptibility to SIV infection.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:114 (abstract no. 98). Unique Identifier : AIDSLINE PRIM8/900098; Rosenberg YJ; White BD; Lewis M; Papermaster SF; Kersey K; de Crombrugghe I; Eddy GA; Burke DS; Henry M. Jackson Foundation, Rockville, MD; Circulating T lymphocytes in primates are usually divided into two subsets: CD45RA+, CD29-, CD45RO- antigen naive resting cells and CD45RAlo/-, CD29+ CD45RO+ memory cells which also express other activation markers e.g. CD44, CD11a (LFA-1) and CD54 (ICAM-1). Using the anti-CD45RA MAb ALB11, a third CD45RAhi subset can
Immunopathogenesis studies on monkeys infected with simian retroviruses.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:115 (abstract no. 99). Unique Identifier : AIDSLINE PRIM8/900099; van Kuyk R; Acevedo R; Munn R; Unger R; Gardner MB; Luciw PA; Department of Medical Pathology, University of California, Davis,; CA 95616; Simian acquired immunodeficiency syndrome (SAIDS) is a fatal disease in rhesus macaques caused by a type D retrovirus designated SAIDS retrovirus (SRV). The SRV-induced syndrome resembles the acquired immunodeficiency syndrome (AIDS) in humans in clinical manifestations and overall immunodeficiency; thus it is a usefu
Natural killer cell activity in HIV-1 infected chimpanzees.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:116 (abstract no. 100). Unique Identifier : AIDSLINE PRIM8/900100; Javadian A; Couch R; Lafrado L; Department of Biochemical Toxicology, Primate Research Institute,; New Mexico State University, P.O. Box 1027, Holloman AFB, NM; 88330; We evaluated in vitro the natural killer (NK) cell activity of human immunodeficiency virus type-1 (HIV-1) infected chimpanzees. Peripheral blood mononuclear cells from HIV-1 infected chimpanzees were separated from whole heparinized blood by Ficoll-hypaque density gradient centrifugation. To evaluate the NK cell acti
Investigations of viral cofactors: molecular interactions of type D simian retrovirus (SRV-1) with cytomegalovirus, simian immunodeficiency virus and simian foamy virus.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:117 (abstract no. 101). Unique Identifier : AIDSLINE PRIM8/900101; Unger RE; Barry PA; Pratt-Lowe E; Luciw PA; School of Medicine, Department of Medical Pathology, University; of California, Davis, CA; Strong evidence supports the notion that cytomegalovirus ( CMV ) may be a cofactor for disease progression in simian immunodeficiency virus (SIV) infected rhesus macaques. SRV-1 like SIV, induces a fatal immunosuppressive disea
The transactivator of human foamy virus maps to the BEL1 genomic region.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:118 (abstract no. 102). Unique Identifier : AIDSLINE PRIM8/900102; Rethwilm A; Erlwein O; Baunach G; Maurer B; ter Meulen V; Institute f\uur Virologie, Versbacher Str. 7, 8700 W\uurzburg,; F.R.G.; The genome organization of human foamy virus (HFV) is characterized by three open reading frames (ORFs) between env and 3 LTR (bel1-3) which are additional to the classical retroviral gag, pol and env genes. Amino acid homology to HIV-2 regulatory genes indicate that the bel ORFs might encode for regulatory proteins s
Retroviral screening service at the London Zoo.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:119 (abstract no. 103). Unique Identifier : AIDSLINE PRIM8/900103; McCLure MO; Simpson GR; Kluge JD; Lerche N; Chrystie IL; Ali M; Stott J; Kirkwood JK; Marx P; Weiss RA; Chester Beatty Labs, ICR, London, UK; A retroviral screening service was offered to the London Zoo and to other institutions housing captive primates. No animal was bled specifically for this exercise. In the case of the London Zoo, a heparinised blood sample (less than 5 ml) was taken from every Old World and New World primate admitted to the Zoo hospita
Natural transmission of SRV-2/Wa in M. nemestrina in an infant primate nursery.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:120 (abstract no. 104). Unique Identifier : AIDSLINE PRIM8/900104; Thouless ME; Moazed TC; Kuller L; Tsai CC; Regional Primate Research Center SJ-50, University of Washington,; Seattle, WA 98195; Thirty-one percent of 113 Macaca nemestrina in the infant nursery at the UWRPRC became antibody positive by ELISA to SAIDS Retrovirus Type D (SRV) in 1988. With the ultimate objective of preventing the natural transmission of SRV in this nursery, a study was initiated to monitor infants in the nursery on a monthly bas
Phenotypic characterization of Kaposi's sarcoma-like tumor associated with SAIDS-D retrovirus in pigtailed macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:121 (abstract no. 105). Unique Identifier : AIDSLINE PRIM8/900105; Tsai CC; Wu H; Tsai CC; Regional Primate Research Center, University of Washington,; Primate Field Station, Medical Lake, WA; D-type simian retrovirus serotypes 1 and 2 (SRV-1 and SRV-2) are etiologically linked to the simian acquired immunodeficiency syndrome (SAIDS) in captive macaques in U.S. primate centers. SRV-2 has been identified as the major cause of morbidity and mortality in the pigtailed macaques (Macaca nemestrina) at the Washin
High incidence of SAIDS-D retrovirus-associated neoplasms in pigtailed macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:122 (abstract no. 106). Unique Identifier : AIDSLINE PRIM8/900106; Tsai CC; Regional Primate Research Center, University of Washington,; Seattle, WA; A D-type simian retrovirus serotype 2 (SRV-2) has been identified as the most important etiological agent for morbidity and mortality in pigtailed macaques (Macaca nemestrina) at the Washington Regional Primate Research Center. Macaques infected with SRV-2 show a simian acquired immunodeficiency syndrome (SAIDS). The
Mutagenesis of leucine zipper within transmembrane protein of Mason-Pfizer monkey virus.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:123 (abstract no. 107). Unique Identifier : AIDSLINE PRIM8/900107; Brody B; Hunter E; University of Alabama at Birmingham, Department of Microbiology,; Birmingham, AL 35294; The leucine zipper was initially hypothesized to be a structure involved in protein: protein interactions via interdigitation of leucine side chains. More recently, the actual structure of the leucine zipper has been shown to be a coiled coil, with the leucines providing a hydrophobic environment for interaction. Evid
Determining the role of the gag polypeptide p12 in the assembly and release of the prototype D-type retrovirus, M-PMV.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:124 (abstract no. 108). Unique Identifier : AIDSLINE PRIM8/900108; Sommerfelt MA; Rhee S; Hunter E; University of Alabama at Birmingham, Department of Microbiology,; Birmingham AL 35294; B-type and D-type retroviruses differ from other retroviruses in that they preassemble immature intracytoplasmic A-type particles (ICAPs) within the infected cell cytoplasm. These particles are comprised of Gag-precursor proteins which then migrate to the plasma membrane where they are released by budding. The gag-gen
CD8+ associated suppression of in vitro SRV-1 production is mediated by a non-specific soluble factor.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:125 (abstract no. 109). Unique Identifier : AIDSLINE PRIM8/900109; Lasarow RM; Marx P; Kluge JD; Gardner MB; Department of Medical Pathology, University of California Davis; Lymphocytes with CD8+ phenotypes have been reported to suppress production of SIV and HIV viruses in respective simian and human systems. We have demonstrated a CD8+ associated activity that is capable of suppressing the production of SRV-1 in vitro. Mononuclear cells from peripheral blood (PBMC) of SRV-infected rhesu
Comparative studies of infection of rhesus macaques and African green monkeys with SIVAGM and SIVMAC strains.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:126 (abstract no. 110). Unique Identifier : AIDSLINE PRIM8/900110; Mone J; Short M; Bradford D; Eichberg J; Allan JS; Department of Virology and Immunology, Southwest Foundation for; Biomedical Research, San Antonio, Texas; While SIV strains from naturally infected sooty mangabeys induce AIDS in experimentally infected Asian macaques, little is known of the pathogenicity of the SIVagm viruses which naturally infect African green monkeys. SIVagm is a nonpathogenic virus found in almost 50% of African green monkeys. This study was undertak
A nonhuman primate model for AIDS and AIDS dementia complex.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:127 (abstract no. 111). Unique Identifier : AIDSLINE PRIM8/900111; Rausch DM; Lendvay J; Sharer L; Eiden LE; Murray EA; N.I.M.H.; Juvenile rhesus macaques inoculated with the B670 isolate of simian immunodeficiency virus (SIV) develop AIDS which is similar to the human disease in symptoms, course and outcome, but in general considerably more rapidly. In addition, the B670 isolate shows neurotropism as manifested by evidence of viral encephalitis
Immunodeficiency and lymphoproliferative disease in an African green monkey dually infected with SIV and STLV-I.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:128 (abstract no. 112). Unique Identifier : AIDSLINE PRIM8/900112; Traina-Dorge V; Blanchard J; Martin L; Murphey-Corb M; Delta Regional Primate Research Center, Tulane University,; Covington, LA; Human T cell leukemia virus (HTLV-I) is a type C exogeneous retrovirus associated with adult T cell leukemia. Seroepidemiological evidence suggests almost all species of Old World monkeys are naturally infected with the closely related simian virus, STLV-I. STLV-I can immortalize monkey cells in vitro. However, no dir
Disease in mangabey monkeys associated with simian immunodeficiency virus and simian T-lymphotropic virus type I.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:129 (abstract no. 113). Unique Identifier : AIDSLINE PRIM8/900113; Klumpp SA; Anderson DC; Fultz PN; Ribas JL; Ansari AA; McClure HM; Yerkes Primate Research Center, Emory University, Atlanta, GA; Following the isolation of simian immunodeficiency virus (SIVsmm) from 14 of 15 healthy mangabey monkeys (Cercocebus atys) in a colony at the Yerkes Primate Center, we reviewed necropsy records for all mangabey deaths between 1969 and 1986 for opportunistic infections or other evidence of immunosuppressive disease. Po
Presence of antibodies to SIV in newly imported baboons.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:130 (abstract no. 114). Unique Identifier : AIDSLINE PRIM8/900114; Watanabe R; Kuller L; Morton WR; Benveniste RE; Washington Regional Primate Research Center, University of; Washington SJ-50, Seattle WA 98195; Sixteen Papio cynocephalus were imported form Addis Ababa Ethiopia in April 1990. All the animals were screened for antibodies to Simian Hemorrhagic Fever within one week of arrival and were found to be negative. Two weeks following arrival, one animal developed marked weight los
Transmission of the molecular clone, SIV(Mne) CL8, in macaques.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:131 (abstract no. 115). Unique Identifier : AIDSLINE PRIM8/900115; Clark EA; Kuller L; Tsai CC; Benveniste R; Shearer GM; Clerici M; Ledbetter JA; Gallatin WM; Morton WR; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; Three transmission studies using a molecular clone of SIV(Mne) were undertaken at the University of Washington Regional Primate Research Center. In an initial study, SIV(Mne) CL8 was demonstrated to be infectious in vivo and caused an AIDS-like disease with mortality in pigtailed macaques (Macaca nemestrina). In a sec
Isolation from African sykes' monkeys (Cercopithecus mitis) of a lentivirus related to human and simian immunodeficiency viruses.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:132 (abstract no. 116). Unique Identifier : AIDSLINE PRIM8/900116; Emau P; McClure HM; Isahakia M; Else J; Fultz P; Yerkes Primate Center, Emory Univ., Atlanta, GA 30322; Immunoassay analysis for antibody in sera from 100 captive Sykes monkeys (Cercopithecus mitis) in Kenya showed that 60 had antibodies to human immunodeficiency virus type-2 (HIV-2). Of these positive sera, 59 were confirmed to have antibodies to HIV-2 by radioimmunoprecipitation ass
Naturally occurring SIV infection in a colony of Macaca arctoides.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:133 (abstract no. 117). Unique Identifier : AIDSLINE PRIM8/900117; Anderson DC; Fultz PN; Ansari AA; Klumpp SA; Ribas JL; McClure HM; Yerkes Primate Research Center, Emory University, Atlanta, GA; A colony of Macaca arctoides at the Yerkes Center was established in 1964 and was maintained as a closed colony until 1981 when four animals obtained from another colony were introduced into the group. One animal died shortly after introduction due to fight wounds. The three other animals remained in the colony until
Persistent HIV 2 infection of rhesus monkey.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:134 (abstract no. 118). Unique Identifier : AIDSLINE PRIM8/900118; Nicol I; Livartowsky J; Chamaret S; Dwyer D; Montagnier L; Dormont D; Commissariat \ga l'Energie Atomique and Centre de Recherches du; Service de Sant\ae des Arm\aees, Fontenay aux Roses, France; OBJECTIVE: To establish an animal model for human HIV infection. Methods: Inoculation: Eight rhesus monkeys were inoculated 3 years ago first IV and IC by 107 in vitro HIV2 ROD-infected cells, then, 1 year ago, IV reinoculated with supernatant of PBL coculture of an infected monkey which presented clinical signs of AI
Virological, immunological and clinical evaluation of Gibbon apes (Hyblobates lar) four years after experimental infection by HIV-1IIIB.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:135 (abstract no. 119). Unique Identifier : AIDSLINE PRIM8/900119; Markham P; Gard E; Robert-Guroff M; Franchini G; Lusso P; Gallo R; Advanced BioScience Laboratories, Kensington, MD; In 1986, four Gibbon apes, three of which had been preimmunized with ISCOM preparations containing HIV-1IIIB or HIV-1RF, core proteins were inoculated i.v. with approximately 10(6) tissue culture infectious units of HIV-1IIIB. All four animals rapidly developed antibody to HIV-1 env proteins and an elevated titer to H
Downregulation of CD28 expression by Tat protein from HIV-1.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:136 (abstract no. 120). Unique Identifier : AIDSLINE PRIM8/900120; Kashiwamura SI; Garcia V; Agy M; Gale M; Katze M; Ledbetter JA; Clark EA; Regional Primate Research Center, University of Washington,; Seattle, WA 98195; The tat gene products of immunodeficiency viruses including HIV-1, HIV-2 and SIV are essential for replication and bind to tat-responsive regions at the 5 of the viral RNA leading to transactivation of genes expressed from the HIV or SIV promoters. Tat may also regulate viral gene expression by post-transcriptional m
Phenotype associated sequence variation both within and around the fusion domain of clonal HIV-1 isolates with varying syncytium inducing capacities.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:137 (abstract no. 121). Unique Identifier : AIDSLINE PRIM8/900121; Andeweg AC; Groenink M; Leeflang P; de Goede RE; Osterhaus AD; Tersmette M; Bosch M; Lab. of Immunobiology, Nat. Inst. of Publ. Health and Env.; Protection, Bilthoven; Immunodeficiency virus-induced syncytium formation is mediated by the fusion peptide located at the amino terminus of the transmembrane glycoprotein. We have shown previously that mutations that change the hydrophobic character of the fusion domain of SIVmac change the ability of the envelope proteins to induce syncyt
Initial characterization of the reverse transcriptase of a human intracisternal A-type retroviral particle associated with Sj\uogren's Syndrome.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:138 (abstract no. 122). Unique Identifier : AIDSLINE PRIM8/900122; Hart DJ; Garry RF; Department of Microbiology and Immunology, Tulane Medical School,; New Orleans, LA 70112; Individuals infected with HIV or SIV often develop symptoms that resemble classic autoimmune diseases. Sj\uogren s Syndrome (SS), also known as autoimmune exocrinopathy, is characterized by dry eyes and mouth, and the infiltration of lymphoid cells into salivary and lacrimal glands. Similar symptoms and glandular path
Psychosocial factors and disease progression in SAIDS: a preliminary report.: Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:139 (abstract no. 123). Unique Identifier : AIDSLINE PRIM8/900123; Capitanio JP; Lerche N; Department of Psychology, University of California, Davis, CA; 95616; SIV infection in macaques, like HIV infection in humans, is characterized by a variable time course to progression to clinical disease. A considerable body of research conducted with humans has implicated psychosocial factors in progression in diseases such as cancer and coronary disease. More recently, persisting imm
Alga associated with diarrhea in AIDS patients and in travelers.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:118 (abstract no. D-227). Unique Identifier : AIDSLINE ASM90/905001; Long EG; Ebrahimzadeh A; White EB; Swisher B; Callaway CS; Centers for Disease Control, Atlanta, GA; Spherical bodies resembling coccidian oocysts and measuring 8.0 - 9.0 um in diameter, were seen in stools from 8 persons with explosive, watery diarrhea. Seven of these patients had traveled to tropical countries, mostly in the Carribbean, and 4 of the patients suffered from AIDS. The structures were easily discernibl
Lack of in vitro activity of silver sulfadiazine against HIV.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:18 (abstract no. A-103). Unique Identifier : AIDSLINE ASM90/905002; Sawyer L; Weber P; Hanson CV; California Dept. of Health Services, Berkeley, CA; Up to 10% of burn patients admitted to an urban medical center have been shown to be positive for HIV. The care of these patients may entail frequent exposure to large amounts of blood and body fluids, placing health care workers at increased risk. Silver sulfadiazine (SSD), a topical antimicrobial agent, is used exte
In vitro activity of CI-978 (AT-4140) against AIDS-derived strains of mycobacterium avium complex (MAC).: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:6 (abstract no. A-31). Unique Identifier : AIDSLINE ASM90/905003; Yajko DM; Nassos PS; Sanders CA; Hadley WK; Univ. of California, San Francisco Gen. Hosp. Med. Ctr., San; Francisco, CA; At present, there is no known clinically effective therapy for treatment of disseminated MAC infection in patients with AIDS. Some strains of MAC are susceptible to quinolone antimicrobial agents, with ciprofloxacin (CIP) being the most active. We compared the in vitro activity of CIP with that of a new fluoroquinolon
Characterization of mycobacterium avium complex isolates from AIDS patients by multilocus enzyme electrophoresis.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:145 (abstract no. U-26). Unique Identifier : AIDSLINE ASM90/905004; Yakrus M; Reeves M; Centers for Disease Control, Atlanta, GA; Nontuberculous mycobacteria belonging to the Mycobacterium avium complex are major opportunistic pathogens isolated from AIDS patients. Sero-agglutination has demonstrated that most isolates are either serotype 4 or 8. Direct transmission has not been documented, and infection is probably caused by either environmenta
Possibility for rapid diagnosis of tuberculous meningitis in HIV positive and AIDS patients by FPEC-GLC analysis of CSF.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:146 (abstract no. U-32). Unique Identifier : AIDSLINE ASM90/905005; Brooks J; Daneshvar M; Johnson A; Fields B; Centers for Disease Control, Atlanta, GA; Frequency-pulsed electron-capture gas-liquid chromatography (FPEC-GLC) techniques for detection of free (non-bound) carboxylic acids were applied to the analysis of trichloroethanol esterified acidic chloroform extracts of serum and cerebrospinal fluid (CSF) from 16 patients with human immunodeficiency virus (HIV) and
Incidence of M. tuberculosis in blood cultures from intravenous drug users with AIDS.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:152 (abstract no. U-67). Unique Identifier : AIDSLINE ASM90/905006; Delfin H; Lue YA; Torres J; Berrios A; Auguste P; Luten IE; Lincoln Medical Center, New York Medical College, Bronx, New York; In 1988 acid fast bacilli (AFB) were isolated from 202 patients, at Lincoln Medical and Mental Health Center. Both bone marrow and blood cultures of eight of these patients and only the bone marrow of one patient grew AFB. M. tuberculosis (MTB) was isolated from blood cultures of 2 patients. One patient had a history
Mycobacterium tuberculosis bacteremia.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:152 (abstract no. U-68). Unique Identifier : AIDSLINE ASM90/905007; Tonnessen G; Slim J; Smith L; Guarton A; St. Michael's Medical Center, Newark, N.J.; Since the Bactec system 460 has been introduced for the isolation of mycobacteria from blood culture we identified 18 patients with MTB bacteremia at an inner city hospital in Newark. We are presenting the epidemiological, clinical laboratory findings and outcome of these patients in a retrospective chart review study
Ultrastructural evidence of conjugation in the life cycle of Pneumocystis carinii.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:219 (abstract no. J-12). Unique Identifier : AIDSLINE ASM90/905008; Itatani C; Mack D; Marshall GJ; California State University, Long Beach; Pneumocystis carinii (PC) is an important opportunist of the immunosuppressed condition, whose life cycle and taxonomic position have not been established. We have found evidence of conjugation in PC comparable to that seen in the yeast, Saccharomyces cerevisiae. Rats with fully developed PC pneumonia were injected wi
Detection of enzymatic activity in pneumocystis carinii using 4-Methylumbelliferyl substrates.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:228 (abstract no. K-50). Unique Identifier : AIDSLINE ASM90/905009; Shaw MM; Paulsrud JR; Bartlett MS; Smith JW; Indiana University School of Medicine, Indianapolis, IN; A method of yeast enzyme detection using 10 4-methylumbelliferyl substrates was applied to P. carinii from culture and from rat lung. Substrates are hydrolyzed by preformed enzymes to produce 4-methylumbelliferone which fluoresces when exposed to light of 365 nm. The 10 substrates used were: beta-D-fucoside, beta-L-fu
A semi-automated assay to screen microbial metabolites for inhibitors of HIV-1 protease.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:263 (abstract no. O-1). Unique Identifier : AIDSLINE ASM90/905010; Lingham RB; Hsu A; Darke P; Koch G; Huang L; Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065; The human immunodeficiency virus (HIV), the etiological agent of the acquired immunodeficiency syndrome (AIDS), selectively infects the immune system rendering the host susceptible to opportunistic infections and neoplasms. The HIV protease participates in the viral life cycle by processing the core protein of 55 kDa
Development of a gene probe procedure for the detection of HIV in hospital wastewaters.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:324 (abstract no. Q-218). Unique Identifier : AIDSLINE ASM90/905011; Richardson KJ; Abbaszadegan M; Rose JB; Univ. of Arizona, Tucson, AZ; Since the recognition of Acquired Immune Deficiency Syndrome (AIDS), the water industry has been concerned with the possible presence of Human Immunodeficiency Virus (HIV) in hospital wastewaters, primarily through the disposal of blood, semen, urine and other contaminated waste to the sewer. Using seeded studies, met
Cytomegalovirus infection as a candidate cofactor in AIDS.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:337 (abstract no. T-7). Unique Identifier : AIDSLINE ASM90/905012; Smith K; Ratner J; Boswell N; Ludwig J; The University of Texas Health Science Center at San Antonio, San; Antonio, TX; The purpose of this long-term study is to test the hypothesis that coinfection with human immunodeficiency virus (HIV) and cytomegalovirus ( CMV ) results in more rapid progression of immunosuppression and AIDS than infection w
Blind perioral esophageal brushing versus esophagogastroduodenoscopy in AIDS patients with esophagitis.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:337 (abstract no. T-8). Unique Identifier : AIDSLINE ASM90/905013; Soroko T; Scrobola C; DePasquale J; Perez G; Saint Michael's Medical Center, Newark, NJ, USA; Blind perioral esophageal brushing is an easy and inexpensive diagnostic tool in isolating microorganisms from suspected cases of esophagitis. We studied ten patients with AIDS that had symptoms of esophagitis not improving after two weeks of oral ketoconazole therapy. Patients signed an informed consent and had blind
Tissue distribution of p24 antigen in HIV-infected athymic mice.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:337 (abstract no. T-9). Unique Identifier : AIDSLINE ASM90/905014; Eiring A; McLaughlin RL; Wetherall NT; Vanderbilt University School of Medicine, Nashville, TN; Irradiated immunedeficient (athymic) mice are capable of supporting replication of HIV. Antigen levels in plasma vary over time, peaking at 6 weeks and remaining elevated for at least 9 weeks. We investigated the levels of p24 antigen in kidney, spleen, and tumor tissue at various time points during a nine week study.
In vitro infection of lamina propria mononuclear cells (LPMC) with human immunodeficiency virus (HIV): evidence of increased viral replication.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:337 (abstract no. T-10). Unique Identifier : AIDSLINE ASM90/905015; Yen-Lieberman B; Sierra-Madero J; Proffitt MR; Fiocchi C; Cleveland Clinic Foundation, Cleveland, Ohio 44195; HIV infection of the gastrointestinal mucosa can be demonstrated in some patients with HIV enteropathy. HIV infected LPMC have been identified in some patients, although the pathogenic significance of this finding is unclear. Perhaps LPMC represent the first cellular targets encountered by HIV during anal intercourse.
Intestinal malabsorption: association with HIV and SIV infections in human and simian AIDS.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-11). Unique Identifier : AIDSLINE ASM90/905016; Dandekar S; Heise C; Martfeld D; Miller C; Marx P; Donovan R; Halsted C; Department of Internal Medicine and California Primate Research; Center, University of California, Davis, CA 95616; Diarrhea and weight loss are frequent manifestations of HIV infection and often occur without any detectable enteric pathogens. The pathogenesis of intestinal abnormalities is not well defined. To understand the etiology of intestinal malabsorption in ARC and AIDS, clinical, pathological, morphometrical, functional, v
Human immunodeficiency virus (HIV-1) infection in rabbits as a model for therapy.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-12). Unique Identifier : AIDSLINE ASM90/905017; Carlson JR; Dickover RE; Dandekar S; Jennings MB; Donovan RM; School of Medicine, Univ. California, Davis; HIV-I infections in rabbits may prove to be an important model. We report the results of experiments that confirm the susceptibility of rabbits for HIV-1. Ten New Zealand White rabbits were challenged with cell-associated HIV-1. The rabbits were tested by a newly developed, qua
Protection for macaques against SIVmac challenge using an inactivated whole virus vaccine.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-13). Unique Identifier : AIDSLINE ASM90/905018; Carlson JR; McGraw TP; Keddie E; Jennings MB; Vowels B; Gardner MB; Med. Pathology, Univ. California, Davis; Rhesus monkeys were immunized by multiple inoculations with purified, inactivated SIVmac (ISIV). Experimental groups included animals that received the SIVmac immunogen in muramyl dipeptide, incomplete Freund s adjuvant, or in aqueous suspension. ISIV immunized animals developed anti-SIV antibodies and positive, SIV s
Protection of zidovudine (AZT)-induced bone marrow toxicity and potentiation of anti-HIV activity with vitamin E.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-14). Unique Identifier : AIDSLINE ASM90/905019; Gogu SR; Rangan SR; Agrawal KC; Dept. of Pharmacol., Tulane Univ. Sch. Med. New Orleans, LA 70112; Significant dose-related anemia has been a limiting factor in the treatment of AIDS patients with AZT . We have investigated the ability of vitamin E (a) to stimulate erythroid progenitor cells, and (b) to inhibit HIV replication. Alpha-D-Tocopherol su
Vaginal transmission of retroviral immunodeficiency disease in mice.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-15). Unique Identifier : AIDSLINE ASM90/905020; Traun K; Hopkins W; Univ. of Wisconsin Med. Sch., Madison, Wisconsin 53792; An acquired immunodeficiency disease is induced when the murine leukemia virus LP-BM5, a retrovirus, is injected intraperitoneally (IP) in mice. We have challenged C57B1/6 mice with LP-BM5 via injection into the peritoneal cavity or inoculation of the vaginal introitus. The vaginal mucosal mode of infection serves as
Human monoclonal antibody reactive with the envelope glycoprotein (gp 120) of HIV-1.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:338 (abstract no. T-16). Unique Identifier : AIDSLINE ASM90/905021; Myers RA; Falkler WA Jr; Patel JD; Joseph JM; State of MD Dept. of Health & Mental Hygiene, Lab. Adm. and Univ.; of MD Dental School, Baltimore, MD; The development of human monoclonal antibodies (HmAb) may be useful in the characterization of the host immune response during infection with HIV-1. HmAb would allow the elucidation of biologically relevant epitopes and may have potential diagnostic or therapeutic applications. Peripheral lymphocytes from an HIV-1 inf
In vivo sequence heterogeneity of human immunodeficiency virus env sub-regions.: Abstr Annu Meet Am Soc Microbiol. 1990 May 13-17;90:340 (abstract no. T-24). Unique Identifier : AIDSLINE ASM90/905022; Howell RM; Fitzgibbon JE; Noe M; Ren Z; Gocke D; Schwartzer TA; Dubin DT; UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ; Sequence analysis of cloned HIV proviral DNA has shown that HIV isolates consist of many molecular variants (e.g., Goodenow et al, J. Acq. Imm. Def. 2