Gay Men's Health Crisis "Treatment Issues" Vol. 8, No. 11 - December 1994
* The best smears are obtained mid-cycle.
* Smears taken during a period or when bleeding for any other reason are unacceptable.
* Patients should not douche, use tampons or have sexual intercourse for 48 hours before a Pap smear.
* Pap smears require two tissue samples. First, clinicians should trace out the squamocolumnar junction (where the inner, more tender and outer, plate-like cervical linings meet) and sample it with a cervical spatula.
* Clinicians also need to obtain an endocervical (inner cervix) sample with a cotton swab or narrow "cytobrush."
* Lubricants interfere with obtaining proper tissue samples.
* As a preservative measure, it is necessary to apply fixative within ten seconds after spreading samples on microscope slides.
* Proper labeling of slides is the clinician's responsibility.
* Patients and clinicians should only accept Pap smear results from a lab that uses the standardized Bethesda system to report results.
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