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5th International AIDS Society Conference on HIV Pathogenesis and TreatmentCape Town - July 19 - 22, 2009 |
ANTIGEN-PRESENTING CELLS REPRESENT POTENTIAL TARGETS FOR R5 TROPIC HIV-1 INFECTION IN THE FIRST TRIMESTER PREGNANCY HUMAN UTERINE MUCOSA
IAS Conf HIV Pathog Treat 2009 Jul 19-22;5th: Abstract No. MOAA204
R. Marlin
1, M.-T. Nugeyre1, C. de Truchis2, N. Berkane3, A. Gervaise2, F. Barré-Sinoussi1, E. Menu1
1Institut Pasteur, Regulation of Retroviral Infection Unit, Virology, Paris, France, 2A. Béclère Hospital, AP-HP, Gynecology-Obstetric Service, Clamart, France, 3Tenon Hospital, AP-HP, UMPC, Gynecology Obstetrics and Reproductive Medecine, Paris, France
BACKGROUND: During the first trimester of pregnancy, HIV-1 mother-to-child transmission (MTCT) is relatively rare despite the permissivity of placental cells to cell-to-cell HIV-1 infection. The invasive placental cells interact directly with the decidual cells (cells of the maternal uterine mucosa during pregnancy) in the first months of pregnancy; however the role of the decidua in the control of HIV-1 transmission remains unknown. The decidua has a characteristic leukocyte population containing natural killer cells (dNK), antigen-presenting cells (dAPC) and T lymphocytes. The aim of this study was to determine whether decidual cell subpopulations could be potential targets for HIV-1 infection, which in this case, should be control in vivo to ensure the protection of the fetus against viral infection.
METHODS: To determine the permissivity of decidual tissue to HIV-1 infection, we adapted and validated a histoculture model. Deciduas were obtained from HIV-1 negative women undergoing elective abortions between 6-10 weeks of pregnancy. Infections were performed in vitro with HIV-1 primary isolates. Infected cells were identified by flow cytometry analysis of isolated decidual mononuclear cells using CD3, CD14, CD56 and antigen-presenting cell markers specific monoclonal antibodies.
RESULTS: Decidual tissue is more susceptible to infection by an R5 tropic HIV-1 (BaL) than an X4 tropic HIV-1 (LAI). Moreover, we show that the level of infection depend on the type of decidua. The results show that subpopulations of dAPC expressing CD14 are the main target of HIV-1 infection in the decidua. They highly express DC-SIGN, CD11b, CD11c and CD16 markers as well as CD80, CD83 and CD86 at a lower level.
CONCLUSIONS: The permissivity of decidual tissue to HIV-1 infection in vitro suggest that in vivo a first level of control of HIV-1 in utero mother-to-child transmission occurs, preceeding and in addition to the control previously demonstrated by the placenta.
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2009-07-22
MOAA204
Oral Abstract Session: MOAA2 - Subversion of Immune Responses in HIV Infection
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