AEGiS-15IAC: Expression of Interlukin-2Rialpha Chain (CD25) on Peripheral Blood Lymphocytes in HTLV-I Associated Myelopathy/ Tropical Spastic Paraparesis Patients.

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

Expression of Interlukin-2Rialpha Chain (CD25) on Peripheral Blood Lymphocytes in HTLV-I Associated Myelopathy/ Tropical Spastic Paraparesis Patients.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. B10078)

Nobahar V, Farid R, Farid F, Abbaszadegan M, Mahmoudi M, Rasteen M
Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran

BACKGROUND: Mashhad, a city in northern Iran, is a newly recognized endemic area for a retrovirus, the Humsn T-cell Lymphotropic Virus Type I (HTLV-I). This virus is the causative agent of a chronic slowly progressive cord syndrome called HTLV-I associated with Humsn T-cell Lymphotropic Virus Type I-asoociated myelopathy or/ tropical spastic paraparesis (HAM/TSP) and has tropism for CD4+ T-cells which results in T-cell activation that escape the autocrine pathway. The purpose of this study was to determine in the immuno-phenotypic features of peripheral blood lymphocytes of HAM/TSP patients, especially differential expression of interleukin-2 receptor a (IL-2Ra) chain on the surface of the T-cells of HAM/TSP patients, HTLV-I carries and healthy controls.

METHODS: Subjects in this case-control study included 20 HAM/TSP patients, 14 HTLV-I carriers and 12 healthy controls. The absolute white blood cell count and the differential cell count were determined by hematologic analysis and the relative and absolute number of peripheral blood CD3+ CD25+ T-cells were determined by flowcytometry.

RESULTS: The relative number of lymphocytes (36±9%), relative number of CD3+ cells (74±7%) and the relative and absolute number of CD25/3+ cells (21±8%, 0.309±0.155*109 /1) were significantly higher in HAM/TSP patients than healthy controls (29±7%, 68±4%, 13±3%, 0.187±0.065 *109 /1) (P<0.05). The relative lymphocyte (36±5%) and relative CD25/3+ (18±5%) cell counts were higher in carriers in comparison with controls. No significant differences were present in these parameters between carriers and HAM/TSP patients.

CONCLUSION: This differential pattern of pattern of T-cell activation markers among the study groups may have striking diagnostic and therapeutic implications.

Keywords: AEGIS, Paraparesis, Tropical Spastic, Receptors, Interleukin-2, Human T-lymphotropic virus 1, Lymphocytes, HTLV-I Infections, T-Lymphocytes, Lymphocyte Activation, Antigens, CD3, Carrier State, Receptors, Interleukin, Leukocytes, Mononuclear, Case-Control Studies, Iran, interleukin-2 receptor alpha, Humans, immunology, surgery, blood

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B10078