AEGiS-15IAC: HLA class I and class II in people living with HIV/AIDS in Chiang Mai province of Northern Thailand.

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

HLA class I and class II in people living with HIV/AIDS in Chiang Mai province of Northern Thailand.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10460)

Kunachiwa W, Leetrakool N, Dettrairat S, Kohreanudom S, Pultajux U, Raksakulkarn P
Faculty of Associated Medical Sciences Chiang Mai University, Chiang Mai, Thailand

BACKGROUND: The association between certain HLA types has been demonstrated in HIV infected patient but results were often inconsistent mainly due to sample size and origin. In order to determine whether HLA polymorphism influences HIV susceptibility, blood samples from HIV-positive people living in Northern Thailand were typed for HLA-A, -B, DRB1 and DQB1.

METHODS: Blood from 95 people living with HIV/AIDS was collected. Serological typing of class I HLA-A and -B by microlymphocytotoxicity and DNA typing of class II HLA-DRB1* and DQB1* using sequence specific oligonucleotide (SSO) reverse line blot hybridization technique were performed. HLA antigens or alleles detected were compared to 130 HIV negative individuals who live in the same area.

RESULTS: Thirteen HLA-A, 20 HLA-B antigens, 22 DRB1* and 10 DQB1* alleles were detected. The results showed that HLA-A11, -A2, -A33, A24, -B46, -B60, -B13, DRB1*15, *16, *1202, *09012, DQB1*02, *03 and *05 have been identified by antigen frequency more than 10%. HLA-A11 was found higher (43.2% vs 30.4%, p=0.0052); specifically HLA-A11.1 (36.84% vs 27.31%, p=0.0303) among the HIV-positive people. None of HLA-B, HLA-DRB1* and -DQB1* was statistically significant, with only increasing HLA-B61 (5.79% vs 2.31%, p=0.056) reported in HIV-positive people.

CONCLUSION: HLA and other host genes are found to be associated with the outcome of HIV infected patient. The association studies reported are discordance because highly polymorphic and ethnic diversity of the population. HLA typing of people in Chiang Mai province of Northern Thailand was documented and be basically benefit for vaccine trial in the future.

Keywords: AEGIS, HLA Antigens, Thailand, Acquired Immunodeficiency Syndrome, HIV Seropositivity, HLA-B Antigens, HLA-DR Antigens, HLA-A Antigens, Alleles, Polymorphism, Genetic, Histocompatibility Testing, HIV Infections, HLA-B61, HLA-DRB1, HLA-A11, Humans, immunology, genetics

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A10460