AEGiS-15IAC: Genetic diversity of HLA-A, -B, -Cw alleles among HIV-uninfected and HIV-infected individuals in a Chinese Uygur ethnic groups.

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

Genetic diversity of HLA-A, -B, -Cw alleles among HIV-uninfected and HIV-infected individuals in a Chinese Uygur ethnic groups.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10380)

Hong KX, Lu XZ, Xu MY, Chen JP, Ruan YH, Xing H, Rui BL, Zhang YZ, Shao YM
National Center for AIDS Control and Prevention, Beijing, China

BACKGROUND: The genes of the major histocompatibility complex (MHC) are the most polymorphic of all human genes. This polymorphism may result in amino acid variation in the peptide-binding groove, which determines the immune response elicited. Certain HLA class I antigens have been reported to be associated with susceptibility to HIV infection or with slow disease progression in some populations. This study is to explore the distribution of HLA-A, -B, -Cw alleles in a Chinese Uygur Ethnic Group and their associations with HIV infection.

METHODS: 119 unrelated healthy subjects and 102 HIV-infected subjects from a Chinese Uygur Ethnic Group volunteered to participate in this study. HLA-A, -B, -Cw genotypings were performed using PCR-SSP method. The gene frequencies and associations between HLA class I antigens and susceptibility or resistance to HIV-1 infection were analyzed by using PPAP and SPSS software.

RESULTS: We identified 18 HLA-A, 28 HLA-B and 22 HLA-Cw specificities in 119 healthy subjects from a Chinese Uygur Ethnic Groups. Among them, A*01, A*02, A*24, B*13, B*15, B*51 and Cw*01, Cw*04, Cw*06, Cw*07 were the most common specificities with the frequency of 0.1134, 0.2185, 0.1345, 0.0955, 0.1136, 0.1136 and 0.1261, 0.1345, 0.1765, 0.1891 respectively. The results of test for Hardy-Weinberg equilibrium showed that the genotype distributions in these three loci were correspondent with expected. Preliminary results showed that the frequency of HLA-B*38 (P=0.04, OR=3.12 ), -Cw*12 (P=0.022, OR=4.203 )were significantly higher, HLA-B*40 (P=0.02, OR=0.39 ) was significantly lower in the 102 HIV infected Uygur individuals when compared to 119 non-infected control subjects.

CONCLUSION: The present study provides the data of HLA-A, -B, -Cw allele frequencies in Chinese Uygur ethnic groups. Some potential HLA specificities associated with susceptibility or resistance to HIV infection are explored.

Keywords: AEGIS, Acquired Immunodeficiency Syndrome, HLA Antigens, Alleles, HIV Seropositivity, Variation (Genetics), Gene Frequency, HLA-B Antigens, Asian Continental Ancestry Group, HIV Infections, Histocompatibility Antigens Class I, Ethnic Groups, Polymorphism, Genetic, Genotype, Disease Progression, DNA Probes, HLA, Humans, immunology, genetics

040711
A10380