AEGiS-15IAC: Human Leukocyte Antigen- associated susceptibility to acquired immune deficiency syndrome (AIDS).

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

Human Leukocyte Antigen- associated susceptibility to acquired immune deficiency syndrome (AIDS).

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10180)

Eglite E, Kovalchuk L, Sochnevs A, Viksna L, Rozentale B, Sture G
RSU Institute of Immunology, Riga, Latvia

BACKGROUND: The aim of this study conducted to look for an association between HLA classes II DRB1, DQA1 and DQB1 locus in predisposition or progression to acquired immune deficiency syndrome (AIDS). During an infection, binding of peptides from the infectious pathogens to HLA proteins is the first step for the initiation of the host-specific immune response. This binding step is critical, because HLA acts as a filter driving the recognition of epitopes by the host immune system. One of the AIDS hypotheses explains the greater inclination to the given pathology by the presence of HLA haplotypes, associated with the higher reactivity of immune system.

METHODS: 185 patients with AIDS from the Latvia were in this study. We analysed the history case of who had been affected by homogenous patient group. All patients at the moment of the initial reference had A-I a stage. We investigated the association of HLA-DRB1; DQB1 and DQA1alleles with rapid progression to AIDS. HLA typing low-resolution for HLA- DRB1*; DQB1*; DQA1*- was performed by polymerise chain reaction (PCR) with amplification with sequence-specific primers (SSP). PCR products were separated on 3% agarose, the amplified bands were visualised, and the DRB1 type was deduced.

RESULTS: The greatest increase of frequency of AIDS- diseases was found in the case of DRB1*01 (odds ratio OR=1.64 p=0,05*), DRB1*08 (OR=3.55, p=0.042*) and DQB1*0201-2 (OR=3,47, p= 0,002), DQB1*0501 (OR=4,03 p= 0,005) and HLA-DQA1*0102 and DQA1*0501 (OR=2.8) alleles frequencies indicate a positive association with rapid course of AIDS disease.

CONCLUSIONS: The data presented here suggests that the identification of class II allele may provide an insight into the molecular mechanism of HIV infection progression and may be useful tool in predicting the clinical outcome in-patients.

Keywords: AEGIS, HLA Antigens, HLA-DQ Antigens, HLA-DR Antigens, Gene Frequency, Haplotypes, Alleles, Disease Susceptibility, Acquired Immunodeficiency Syndrome, HIV Infections, Genetic Predisposition to Disease, Polymerase Chain Reaction, Latvia, HLA-DQA1, HLA-DRB1, Humans, immunology, genetics

040711
A10180