AEGiS-15IAC: Distribution of the CCR5 gene 32-base pair deletion in Russian intravenous drug users.

15th International AIDS Conference


Bangkok, Thailand - July 11-16, 2004

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Distribution of the CCR5 gene 32-base pair deletion in Russian intravenous drug users.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10090)

Lioznov D, Zhevnerova N, Nikolaenko S, Antonova T, Belyaeva T
Pavlov State Medical University, St. Petersburg, Russian Federation

BACKGROUND: A 32-basepair deletion polymorphism in the a-chemokine receptor gene CCR5 (CCR5Delta32) has been identified and shown to have functional significance in determining susceptibility to infection by HIV-1 and possibly in influencing disease progression in HIV-1 positive individuals. This study was carried out to determine prevalence of CCR5-Delta32 allele frequency in high risk HIV-infection population in St Petersburg, Russia.

METHODS: Blood samples were obtained from 36 intravenous drug users admitted to an Inpatient Clinic of Department of Infectious Diseases and Epidemiology, Pavlov State Medical University, St. Petersburg, Russia and 22 controls from the same population. Polymerase chain reaction analysis was performed on genomic DNA samples. HIV status was confirmed by anti-HIV enzyme immunoassay and Western blot analyses.

RESULT: The average age of all subjects was 21.1±3.1 years. All individuals were Caucasian. None was homozygous for CCR5-Delta32. Eleven (19%) individuals were heterozygous. The distribution of heterozygotes was similar in IDUs and non-IDUs. Twenty-three (63,8%) of IDUs were HIV-1 infected individuals. The distribution of heterozygotes were similar in HIV-infected and non-infected IDUs as well.

CONCLUSION: The CCR5-Delta32 heterozygous state seems to be not rare in St. Petersburg population. As well these data do not seem to support a protective role of the CCR5-Delta32 heterozygous state in preventing HIV infection.

Keywords: AEGIS, Receptors, CCR5, Substance Abuse, Intravenous, Gene Deletion, HIV-1, HIV Infections, Base Pairing, Acquired Immunodeficiency Syndrome, Heterozygote, HIV Seropositivity, Gene Frequency, Disease Progression, Polymorphism, Genetic, Russia, genetics

040711
A10090

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