AEGiS-15IAC: Gennotypic drug-resistance testing in early HIV infection.

15th International AIDS Conference


Bangkok, Thailand - July 11-16, 2004

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Gennotypic drug-resistance testing in early HIV infection.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10050)

Prempeh Agyemang PA
Y.W.C.A., Kumasi, Ghana

OBJECTIVE: To measure the prevalence of mutations associated with the reverse transcriptase(RT) and protease gene in patients with recently acquired HIV infection.

METHOD: Patient whose first positive HIV-1 and body test was after january1995 were identified from the Brigton HIV clinic database. Patient were included if they had a documented seroconversion illness or a negative HIV test within 18months of their first positive test. Patient were excluded if they had receiced antiretrovirals(ARVs) before the store sample was collected. Sequencing was undertaken bby ABI automated sequencing with Big Dye terminators.

RESULT:Of 44 patient,36had sufficient store plasma or serum. sequencing of 10 sampred date shows that all patientt had at least one secondary mutation in the protease gene. One patient demonstrated the RTA mutation,a variant usually associated with multi-nucleoside resistance(Q151M) in drug-experienced patients and not previously described in a naïve patient. A second patient displayed a K43Q mutation,which usually occurs in individuals with prior exposure to ZDV and 3TC, together with other mutations.

CONCLUSION: Whilst CD4,viral load,clinical data and resistance genotypes will be presented on the whole cohort, these preliminary results show the potential for transmission of viruses containing mutation not previously described in isolated. It is as yet unclear what effect these will have on future treatment response.

Keywords: AEGIS, HIV Infections, HIV-1, Zidovudine, Viral Load, Drug Resistance, Viral, Prevalence, HIV-1 Reverse Transcriptase, HIV Seropositivity, Acquired Immunodeficiency Syndrome, Drug Resistance, Multiple, Viral, Research Design, Drug Resistance, Drug Resistance, Microbial, Mutation, Humans, genetics

040711
A10050

Copyright © 2004 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.