AEGiS-15IAC: Detection of TT Virus and analyses of partial TTV-DNA sequences in IVDUs infected with HIV.

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

Detection of TT Virus and analyses of partial TTV-DNA sequences in IVDUs infected with HIV.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. A10020)

Zhu B, Wu NP, Liu KZ, Chen YG, Chen Z
The institue of infectious disease, the 1st hospital of Zhejiang University, Hangzhou, China

BACKGROUND: Objective to study the TTV infection rate in IVDUs infected with HIV and the mutation of partial TTV-DNA sequences.

METHODS: TTV-DNA were detected in 35 HIV positive, 64 HIV negative IVDUs and 45 normal blood donors by nested-PCR. The partial genes of TTV-DNA fragments amplified from two HIV positive IVDUs and one HIV negative IVDUs were analysed and then compared with gene from Japan.

RESULTS: TTV-DNA positivities were 25. 71%(9/35) in HIV infected IVDUs,17.18%(11/14) in HIV negative IVDUs and 2.2%(1/45) in normal blood donors. The homology of TTV-DNA sequence are 96.44% and 95.79% in the HIV infected IVDUs and 98.05% in the HIV non-infected IVDUs, comparing with Japanese one. Greater mutation in 299-309 of our detected region was found between HIV positive and HIV negative IVDUs.

CONCLUSIONS: IVDUs has higher TTV infection and HIV infection or HIV without infection couldn't affect the TTV infection,but some mutation of TTV could be found in TTV superinfected HIV IVDUs.

Keywords: AEGIS, Transfusion-Transmitted Virus, HIV, HIV Seropositivity, Acquired Immunodeficiency Syndrome, DNA Virus Infections, HIV Infections, Blood Donors, DNA, DNA, Viral, Polymerase Chain Reaction, Base Sequence, DNA Viruses, Japan, analysis, genetics

040711
A10020