AEGiS-14IAC: Hemi-nested PCR by using TGR1E sequence for detection of Toxoplasma gondii in blood samples from HIV-infected patients in the city of Sao Paulo, Brazil.

14th International AIDS Conference


Barcelona, Spain - July 7-12, 2002

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Hemi-nested PCR by using TGR1E sequence for detection of Toxoplasma gondii in blood samples from HIV-infected patients in the city of Sao Paulo, Brazil.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. B10176)

Gomes VM, Hirata RD, Hirata MH, Yamamoto YI, Soares CL, Kanashiro CK, Daher MA, Souza AM, Yokio S
Clinic and Toxicology Analysis Department of The School of Pharmaceutical Sciences of The University of Sao Paulo, Sao Paulo, Brazil

INTRODUCTION: The reactivation of latent T. gondii cysts is one of the most common opportunistic infection in patients with AIDS.

METHOD: In this study, we have developed a hemi-nested PCR (Henes-PCR) procedure for detection of T. gondii in blood samples of 38 seropositive and 21 seronegative individuals. DNA was extracted by salting-out and chaotropic methods. DNA was carried out by amplification of the TGR1E sequence by PCR and Henes-PCR procedures.

RESULTS: The analytical sensibility of Henes-PCR was greater (102 parasites/ml) than that of PCR (104 parasites/ml). The analysis of blood samples revealed that both amplification procedures showed high specificity (100%). In T. gondii seropositive patients, 84% (32/38) were also positive for HIV, characterized as: 7 - A1, 12 - A2, 1 - A3, 1 - B3, 3 - C2 and 9 patients in C3 clinical category (MMWR 41: RR - 17, Dec. 18, 1992). PCR and Henes-PCR was positive blood samples from 1 of 4 patients from C2 and C3 groups that had not received parasite prophylaxis.

CONCLUSION: These preliminary data indicates that Henes-PCR procedure is a useful tool for the detection of toxoplasma reactivation in HIV- infected individuals.

Keywords: AEGIS, Toxoplasma, Polymerase Chain Reaction, HIV Infections, Acquired Immunodeficiency Syndrome, HIV Seropositivity, Sensitivity and Specificity, Opportunistic Infections, Brazil, Animal, Human, utilizationKWDaegis,toxoplasma,polymerasechainreaction,hivinfections,acquiredimmunodeficiencysyndrome,hivseropositivity,sensitivityandspecificity,opportunisticinfections,brazil,animal,human,utilization

020707
B10176

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