AEGiS-13IAC: Adaptive evolution of human immunodeficiency virus type 1 having common origin within different individuals.

13th International AIDS Conference

Durban, South Africa - July 9-July 14, 2000

Adaptive evolution of human immunodeficiency virus type 1 having common origin within different individuals.

Int Conf AIDS 2000 Jul 9-14; 13:(abstract no. MoOrA164)

Lee J-S, Kim S-S, Koo B-K, Choi B-S, Seong B-M, Suh S-D, Kim O-J, Cho H-W
J.-S. Lee, National Institute of Health, Center for AIDS Research, #5, Nokbun-dong, Eunpyung-gu, Seoul, 122-701, Korea, Republic Of, Tel.: +82 2 380 1511, Fax: +82 2 359 1397, E-mail: jooshil@nih.go.kr

BACKGROUND: To evaluate the evolutionary dynamic under natural selection, the HIV-1 quasispecies virus population from a sexual transmission pair who have different HLA types (husband: HLA A2, A33; HLA B44, B51 and wife: HLA A30; HLA B13, B44) were analyzed.

METHODS: Proviral and cDNA sequences spaning V1 through V5 were amplified by polymerase chain reaction from blood samples obtained during the almost 5 year study. Product DNAs from five to seven time points per individuals were cloned up to 10 clones from each sample and sequenced. Nucleotide variability and phylogenetic tree was constructed. The known sequences of each HLA epitopes were compared.

RESULTS: Phylogenetic analysis of these sequences reveals that the major population of the virus changes according to a individual. All DNA and cDNA sequences from wife except two RNA sequences from early infection stages (less than 2 years after infection) were separated from those of husband. Interperson variation of HIV-1 quasispecies was increased gradually after infection.

CONCLUSIONS: Even though the origin of the virus was common, the adaptive evolution of the HIV-1 quasispecies populations under natural selection lead a quite different major population.

Keywords: AEGIS, HIV-1, Evolution, Base Sequence, Variation (Genetics), Selection (Genetics), Human, genetics
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