AEGiS-12IAC: Recovery of HIV from syringes.

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

Recovery of HIV from syringes.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:386 (abstract no. 23222)

Masters B, Abrishanir M, Farzadegan H
Johns Hopkins University, Baltimore, MD, USA.

OBJECTIVE: To determine if viable HIV virus can be recovered (cultured) after varying dry times, from pre-exposed syringes containing a known quantity of HIV.

METHODS: Nine insulin syringes were inoculated with 10,000 TCID50 of MN laboratory strain of HIV. Each syringe had a designated dry time of either 0, 1 2, 4, 8 and 24 hrs, 1 week, 2 week and 1 month. Syringes were preloaded with sterile PBS, to mimic the presence of drug. Approximately 0.5 cc of viral stock was drawn up into the syringe and a 'booting' procedure was applied for 30 sec., after which all contents of syringe were pushed out. Syringes were stored at room temperature for each designated dry time. Virus was recovered by drawing up 2 cc of culture medium containing one million non-infected peripheral blood mononuclear cells. After 2 minutes contents were pushed out, cultured for 21 days using microculture techniques, and tested for growth by p24 antigen production.

RESULTS: Syringes with dry times up to 24 hours were culture positive, except for the 8 hr dry time. All syringes with dry times longer than 24 hours were culture negative.

CONCLUSION: Based upon the experimental design utilized in this study, laboratory strain of HIV was recovered from syringes with varying dry times up to 24 hours.

Keywords: AEGIS, HIV, Syringes, HIV Infections, HIV Seropositivity, Culture Media, Temperature, methods, ICA12
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