AEGiS-12IAC: V3 sequence diversity of HIV-1 subtype E in infected mothers and their infants.

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

V3 sequence diversity of HIV-1 subtype E in infected mothers and their infants.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:19 (abstract no. 11184)

Foongladda S, Sutthet R, Chearsakul S, Wanprapa N, Likanonsakul S, Wasi C
Microbiology Department, Siriraj Hospital, Nontabure, Thailand.

OBJECTIVE: To elucidate the molecular characteristic of HIV-1 subtype E involved in vertical transmission, especially the genetic diversity of V3 sequences of proviral DNA in mother-infant pairs.

METHODS: Blood specimens were collected from 17 HIV-1 mother-infant pairs during 1994-1995. The first blood collections were taken within 2 months after birth and every 4-6 months for 2 years. All mothers were asymptomatic cases and infected via heterosexual route. The V3 regions of HIV-1 subtype E proviral DNA in peripheral blood mononuclear cells of the first blood collections after cloned into pCRII vector. Three to seven clones of each samples were collected. The 105 nucleotide sequences were translated to the corresponding amino acids and aligned by using DNASIS version 2.2. Pairwise distances were performed by using software ESEE and MEGA.

RESULTS: Eight infants showed mild symptomatic and immunosuppression within the first year of life. The interpatients nucleotide distance of mothers and infants in these group showed more diversity than those of non-immunosuppression group (0.065 ± 0.008 and 0.039 ± 0.006, respectively). There was a significant association between nucleotide distance > 6% of the infants from their mothers with the rapid disease progression of the infants (F-test, p = 0.003). The substitution with asparagine (N) at treonine (T) at position 13 and aspartic acid (D) at position 29 of the V3 sequence were significantly associated with non-immunosuppression in the first year of life of the infants (F-test, p = 0.003).

CONCLUSION: Either a single or more of the viral variants could transmit from mothers to their infants. The mother-infant distance more than 6% associated with the infants who immunosuppression within the first year of life. The N substitution at position 13 or/and 29 of the V3 loop were highly conserved in vertical transmission and significantly association with immuno status of the infants.

Keywords: AEGIS, HIV-1, Disease Transmission, Vertical, Mothers, Base Sequence, Variation (Genetics), Disease Progression, Infant, Human, transmission, genetics, ICA12
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