AEGiS-12IAC: Characterization of a new long-term T-cell line SCHT persistently infected with HIV-1.

12th International AIDS Conference


Geneva, Switzerland - June 28-July 3, 1998

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Characterization of a new long-term T-cell line SCHT persistently infected with HIV-1.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:14 (abstract no. 11159)

Miller GG, Kushch AA, Glukhowa LA, Klimova RR, Titova IV, Pokidicheva LN;;; Inst of Virology, Moskow, Russia.

OBJECTIVES: The persistently infected T-lymphoblastoid cell lines are the manageable experimental models for in vitro studying molecular and cellular mechanisms involved in the occurence and development of the persistent form of HIV infection and pave the way to controlling the foci of persistent HIV infection in people.

METHOD: An investigation of SCHT in dynamic of productive persistent infection. Western blot (WB) analysis of the SCHT viral proteins. Chromosome analysis and Karyotype reconstruction. Detection of a site of HIV genome integration on chromosomes by in situ hybridization. Evaluation of receptor activity of SCHT by monoclonal antibodies (MAB). Estimation of expression and accumulation of HIV structural proteins directed by genes env and pol.

RESULTS: SCHT cell line is able cultivating in vitro unprecedently long period of time without any destruction and without adding uninfected cells to support of infection. Abundant virl production and long-term stable expression of the full-size major structural proteins were detected. Chromosome analysis carry out every 2-3 years was revealed a great stability of its karyotype. All cells are contain of integrated proviral DNA HIV in chromosomal DNA.

CONCLUSION: New type of T-cell line SCHT with high infective viral production and high-level expression of the viral genes without cell death was described. We speculate possibility to development of a non-lytic form of HIV infection in vivo. SCHT cell line is offer as a useful model to search fundamental problem of HIV persistence.

Keywords: AEGIS, HIV-1, T-Lymphocytes, HIV, HIV Infections, HIV Antigens, HIV Core Protein p24, HIV Envelope Protein gp41, HIV Long Terminal Repeat, Cell Line, HIV-1 Reverse Transcriptase, Genes, Viral, In Vitro, immunology, genetics, ICA12KWDaegis,hiv-1,t-lymphocytes,hiv,hivinfections,hivantigens,hivcoreproteinp24,hivenvelopeproteingp41,hivlongterminalrepeat,cellline,hiv-1reversetranscriptase,genes,viral,invitro,immunology,genetics,ica12
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