AEGiS-11IAC: Detection of HHV-8 in tissues of various organs from AIDS-KS patients.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Detection of HHV-8 in tissues of various organs from AIDS-KS patients.

Int Conf AIDS 1996 Jul 7-12; 11:8 (abstract no. We.A.166)
Huang YO, Li JJ, Feiner D, Zhang WG, Cockerell CJ, Demopoulos RI, Friedman-Kien AE; NYU Medical Center, Department of Microbiology, New York, NY, USA. Fax: 212-263-7933.

OBJECTIVE: To study the distribution in different organs of HHV-8 in AIDS-KS patients.

METHODS: Autopsy specimens of various organs from 5 AIDS-KS patients and 6 AIDS patients without KS were collected. DNA were extracted from paraffin sections. PCR was performed in solution containing TAQ, TAQ buffer, dNTP and primer pair specific for HHV-8. PCR products were analyzed by Southern blot hybridization with internal probe.

RESULTS: (table: see text) On the other hand, total 50 samples from various organs from AIDS patients without KS show negative for HHV-8 by PCR.

CONCLUSION: Our results showed that HHV-8 could be detected in several organs such as lymph nodes, lung, stomach, spleen, prostate, esophagus and rectum. Since most of these organs in which HHV-8 could be detected are the organs in which KS often occur, the possible relationship of HHV-8 in the pathogenesis of KS is further suggested.

Keywords: AEGIS, Herpesvirus 8, Human, Acquired Immunodeficiency Syndrome, Polymerase Chain Reaction, Laboratory Techniques and Procedures, Human, Male, ICA11KWDaegis,herpesvirus8,human,acquiredimmunodeficiencysyndrome,polymerasechainreaction,laboratorytechniquesandprocedures,human,male,ica11

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WeA166

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