AEGiS-11IAC: P. carinii DNA detected by ITSs nested PCR in oropharingeal and blood samples of HIV pts with PCP.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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P. carinii DNA detected by ITSs nested PCR in oropharingeal and blood samples of HIV pts with PCP.

Int Conf AIDS 1996 Jul 7-12; 11:230 (abstract no. Tu.B.115)
Atzori C, Agostoni F, Zambelli A, Cargnel A; II Divisione Mal Infettive-Osp L. Sacco, Milano, Italy. Fax: 0039-2-38200909.

OBJECTIVE: To demonstrate the presence of P. carinii DNA in blood (serum and PBMC) and oropharyngeal samples of AIDS pts during acute episodes of PCP by ITSs nested PCR. Subjects and methods: Sterile saline garglings and serial blood samples from 28 AIDS pts with PCP (BAL positive for P. carinii) have been examined by ITSs nested PCR followed by Dot Blot hybridization. The same biological samples collected from 13 healthy subjects and from 8 AIDS pts with other opportunistic diseases were used as controls.

RESULTS: All diagnostic BALs, 49 garglings, 162 sera, (at least 2 for each pt) and 36 Ficoll-separated PBMC were examined. Among the PCP pts, 25/28 (89,2%) had at least one serum, 19/28 (67,85%) PBMC and 21/28 (75%) oropharyngeal sample with detectable P. carinii DNA during after ITS nested PCR. Control samples were all negative by this assay.

CONCLUSIONS: ITSs nested PCR could be used for detection of P. carinii DNA in non invasive samples such as serum, Ficoll separated PBMC or garglings in patients who cannot undergo an invasive procedures such as the bronchoalveolar lavage. A correlation exists between acute PCP and P.c specific DNA signal in the period prior to the beginning of effective therapy. Our data suggest that blood (serum and PBMC) P. carinii DNA is a not-genomic, fragile and probably fragmented molecule, possibly macrophage-derived, and rapidly cleared after the beginning of specific treatment.

Keywords: AEGIS, Polymerase Chain Reaction, DNA, DNA, Fungal, DNA Primers, Bronchoalveolar Lavage Fluid, DNA, Bacterial, Bronchoalveolar Lavage, Sequence Analysis, DNA, HIV Infections, Human, ICA11KWDaegis,polymerasechainreaction,dna,dna,fungal,dnaprimers,bronchoalveolarlavagefluid,dna,bacterial,bronchoalveolarlavage,sequenceanalysis,dna,hivinfections,human,ica11

960707
TuB115

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