AEGiS-11IAC: HHV8 and other herpes virus in AIDS related and endemic Kaposi's sarcoma (KS) and malignant lymphoma.

11th International AIDS Conference

Vancouver, British Columbia — July 7-12, 1996

HHV8 and other herpes virus in AIDS related and endemic Kaposi's sarcoma (KS) and malignant lymphoma.

Int Conf AIDS 1996 Jul 7-12; 11:215 (abstract no. Th.A.272)
Biberfeld P, Ekman M, Kaaya EE, Jagdahl L, Linde A, Biberfeld G; Immunopathology Lab., Karolinska Hospital, Stockholm, Sweden. Fax: 46-8-345 820. E-mail: Peter.Biberfeld@onkpat.ki.se.

OBJECTIVE: To study the prevalence of HHV8 in Swedish and Tanzanian HIV+/- patients with/without Kaposi's sarcoma (KS) (110) or malignant lymphoma (ML) (25).

MATERIAL AND METHODS: Biopsies from involved and non involved tissues including PBMC of men, women, children were studied by PCR for HHV8 DNA (Chang, Y. et al., Science, 266, 1865-, 1994). In situ hybridization was performed on selected cases. Observations: All frozen biopsies of tissues in AIDS and endemic, nodular KS expressed HHV8 DNA. Lesions at patch and plaque stages also expressed HHV8 DNA, but less than the nodular lesions. Approximately 1/3 of AIDS related (African) ML had PCR demonstrable HHV8 DNA, but a few HIV seronegative cases were also positive. Expression of HHV8 in ML was not related to any distinct histopathology, which was either immunoblastic or Burkitt-type or pleomorphic. From in situ hybridization, mostly vascular lining cells and spindle cells of KS appeared to contain HHV8 DNA, whereas in ML it was not possible to incriminate a distinct cell type for HHV8 expression. Most biopsies of African AKS, EKS and ML also contained demonstrable EBV and in some cases also HHV6 DNA.

CONCLUSION: Not only AIDS associated KS but also endemic KS appears consistently associated with HHV8 DNA irrespective of stage, tissue, gender or age. A relatively high prevalence of HHV8 was also seen in African, malignant lymphoma in HIV+ or HIV- patients. HHV8 thus appears to be more prevalent in Central Africa compared to Europe and U.S. The type(s) of cell expressing HHV8 DNA are probably different in KS and ML.

Keywords: AEGIS, Sarcoma, Kaposi, Herpesvirus 8, Human, Herpesviridae, Acquired Immunodeficiency Syndrome, Lymphoma, HIV Infections, HIV, Herpesvirus 4, Human, Simplexvirus, Lymphoma, AIDS-Related, Polymerase Chain Reaction, Herpesviridae Infections, In Situ Hybridization, CD4 Lymphocyte Count, Prevalence, HIV-1, Lymphoma, Non-Hodgkin, Laboratory Techniques and Procedures, Herpesvirus 6, Human, Europe, Africa, Central, United States, Human, Female, Male, Child, ICA11

960707
ThA272