AEGiS-11IAC: Cytolytic T-cell activity in peripheral blood of HIV-1 infected individuals.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Cytolytic T-cell activity in peripheral blood of HIV-1 infected individuals.

Int Conf AIDS 1996 Jul 7-12; 11:211 (abstract no. Th.A.140)
Hinkula J, Leandersson AC, Nordlund S, Geretti AM, Bratt G, Popovic M, Sandstrom E, Wahren B; Swedish Institute for Infectious Disease Control, Department of Virology, Stockholm, Sweden.

OBJECTIVE: To evaluate the cytolytic T-lymphocyte efficacy in patients to their own T- and B-lymphocytes.

METHODS: Cells from asymptomatic (greater than 5 years) HIV-1 infected patients with initial greater than 400x109/l CD4+ T-cell counts were studied. Cytolytic T-cell activity was measured with the Cr51 release assay against the patient's autologous EBV-transformed B- (B-LCL) and HTLV-1 transformed CD4+ T-cells (CD4+ TK). Precursor CTL frequencies were determined by the limiting dilution assay.

RESULTS: Eleven patients were tested for precursor frequency of HIV-1 specific cytolytic T-cells to whole HIV-1 infected targets, (envelope, nef, rev) or target cells with the respective peptides. Presence of direct CTL activity in peripheral blood was measured against whole HIV-1 and nef-expressing target cells. In eight of the studied individuals HIV-1 specific precursor CTL:s were detected in frequencies ranging from 2-84 CTL T-cells/106 peripheral blood mononuclear cells. (table: see text)

CONCLUSIONS: The CTL precursor frequencies vary considerably between infected individuals, without obvious differences in initial clinical status. Autologous HIV-1 presenting B-LCL appear to function better than autologous CD4+ T-cells as targets. The target cells in vivo usually are HIV-infected CD4+ cells, not necessarily eliminated by the in vitro measured CTL activity to HIV-transfected B-cells.

Keywords: AEGIS, HIV-1, T-Lymphocytes, Antigens, CD4, T-Lymphocytes, Cytotoxic, HIV Infections, B-Lymphocytes, CD4-Positive T-Lymphocytes, HIV, T-Lymphocyte Subsets, HIV Seropositivity, Herpesvirus 4, Human, In Vitro, Human, ICA11KWDaegis,hiv-1,t-lymphocytes,antigens,cd4,t-lymphocytes,cytotoxic,hivinfections,b-lymphocytes,cd4-positivet-lymphocytes,hiv,t-lymphocytesubsets,hivseropositivity,herpesvirus4,human,invitro,human,ica11

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ThA140

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.