AEGiS-11IAC: Correlation between neutralizing properties and genomic composition of Mexican HIV-1 isolates.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Correlation between neutralizing properties and genomic composition of Mexican HIV-1 isolates.

Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1011)
Barquet A, Gudino JC, Soler C; Unidad de Investigacion en Retrovirus Humanos. Instituto de Investigaciones Biomedicas, UNAM and Instituto Nacional de Diagnostico y Referencia Epidemiologicos, SSA. Mexico. Fax: 3413264. E-mail: csoler@servidor.unam.mx.

OBJECTIVES: To correlate data from neutralization assays and the V3sequences in 7 strains of HIV-1. METHODS. Neutralization assays were carried out with 43 sera against 500 TCID50 of 7 different viral strains (MEXP2; MEXP4; MEXA3; MEXP42; MEXP42m; MEXP42p and IllB/LAV) using SupT1 as target cells. A 320 bp dsDNA fragment corresponding to V3 was PCR amplified using 1 microgram of DNA extracted from cultured cells infected with the same viral strains used for neutralization assays. The PCR products were purified by the low-melting point agarose method and sequenced directly by the dideoxi-chain terminator method.

RESULTS: In the neutralization assays we found that P2 and P4 strains, isolated from hemophilic patients, were the most neutralized by the anti-HIV-1 positive human sera (40/43); P42, P42m and P42p, isolated from a sexually infected couple and their vertically infected child, were neutralized by 35-36 out of the 43 sera; while A3, (blood transfusion transmission) and Illb/LAV were the less susceptible viruses (25 and 22 out of 43 respectively). To establish the relationship between the neutralization susceptibilities of the strains a Pearson's correlation table with the neutralization data was calculated. A phylogenetic dendograme was constructed with the V3 sequences showing 3 groups of viruses. One including the P42's viruses together with A3; the second group consists of the P2 and P4 viruses; and IIIB/LAV alone in a third group. The neutralization correlation data shows that the P42 isolates are closely related, (Correlation coefficient =0.761) in agreement with the homology of the V3 region between this viruses (82.9%). P4 has a 59% homology in the V3 sequence with A3, P2 and IIIB/LAV and their neutralization data shows a variable correlation of 0.323, -0.075 and 0.280 respectively. Conclusions. We found that the neutralization properties and the V3 sequences homologies do not correlate with each other, except for the P42 viruses, confirming that the neutralization of HIV is a complex phenomena not determined exclusively by PND of the V3 region, and that other epitopes play an important role.

Keywords: AEGIS, HIV-1, HIV Envelope Protein gp120, HIV Antibodies, HIV Antigens, HIV, HIV Infections, HIV Core Protein p24, Epitopes, HIV Seropositivity, Polymerase Chain Reaction, Hispanic Americans, Child, Human, virology, ICA11KWDaegis,hiv-1,hivenvelopeproteingp120,hivantibodies,hivantigens,hiv,hivinfections,hivcoreproteinp24,epitopes,hivseropositivity,polymerasechainreaction,hispanicamericans,child,human,virology,ica11

960707
PubA1011

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