AEGiS-08IAC: SIV-specific cytotoxic T lymphocytes are present in the AIDS-associated skin rash in rhesus monkeys.

8th International AIDS Conference


Amsterdam, Netherlands — July 19-24, 1992

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SIV-specific cytotoxic T lymphocytes are present in the AIDS-associated skin rash in rhesus monkeys.

Int Conf AIDS 1992 Jul 19-24; 8:We53 (abstract no. WeA 1049)
Yamamoto H, Ringler DJ, Miller MD, Yasutomi Y, Hasunuma T, Letvin NL; Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.

OBJECTIVE: An infiltration of CD8+ lymphocytes in the dermis and epidermis underlies the skin rash which commonly occurs as a manifestation of HIV and simian immunodeficiency virus (SIV) infections. We functionally characterized these skin rash-associated lymphocytes in SIVmac-infected rhesus monkeys.

METHODS: Lymphocytes isolated from skin biopsy specimens derived from rashes of SIVmac-infected rhesus monkeys were expanded in interleukin-2-containing medium, cloned by limiting dilution and assessed for functional activity.

RESULTS: Skin rash-associated lymphocytes exhibited greater lysis of SIVmac-expressing target cells and a higher cloning efficiency for SIVmac-specific effector T cells than peripheral blood lymphocytes. Moreover, both SIVmac envelope- and gag-specific cytotoxic T lymphocytes (CTL) could be readily cloned from these skin rash-associated lymphocytes. These skin rash-associated CTL exhibited the same MHC restriction and epitope specificity as those CTL derived from PBL.

CONCLUSIONS: Cutaneous infiltrating CD8+ lymphocytes in SIVmac-infected rhesus monkeys include SIVmac-specific CTL. Thus, CTL play a role in the pathogenesis of skin lesions which occur following this infection.

Keywords: AEGIS, SIV, T-Lymphocytes, Cytotoxic, Macaca mulatta, Simian Acquired Immunodeficiency Syndrome, CD8-Positive T-Lymphocytes, Acquired Immunodeficiency Syndrome, Exanthema, Epitopes, HIV, Skin Diseases, Clone Cells, Animal, ICA8KWDaegis,siv,t-lymphocytes,cytotoxic,macacamulatta,simianacquiredimmunodeficiencysyndrome,cd8-positivet-lymphocytes,acquiredimmunodeficiencysyndrome,exanthema,epitopes,hiv,skindiseases,clonecells,animal,ica8
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WeA1049

Copyright © 1992 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.