AEGiS-07IAC: Neutralization of HIV-1 infection by anti-V3 loop antibody is dependent upon the host cell of the viral inoculum.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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Neutralization of HIV-1 infection by anti-V3 loop antibody is dependent upon the host cell of the viral inoculum.

Int Conf AIDS 1991 Jun 16-21; 7:41 (abstract no. M.A.76)
Ohashi K, Popovic M, Liu Y, Hunter E, Javaherian K, Eddy G, Gartner S; Fujisaki Institute, Okayama, Japan

OBJECTIVE: We sought to determine whether antibodies capable of neutralizing virus infection of T cells can also influence infection of mononuclear phagocytes.

METHODS: HIV-1LW-PBM, an isolate from a lab worker infected with HTLV-IIIB was used. To generate viral stocks, early passage virus was transmitted to monocyte/macrophages (MM), T cells and H9 cells. Goat antisera prepared against peptides corresponding to the V3 loops of HTLV-IIIB, -RF, -WMJ-2, -MN and -SC were tested in comparison with AIDS patients' sera. Viral inocula containing 40 TCID50 were used in microwell neutralization assays, and infection determined by p24 antigen assay.

RESULTS: Antibodies against V3 loop peptides as well as AIDS patient's sera were able to neutralize infection of H9 cells regardless of whether the viral inoculum was derived from normal T cells, MM or H9 cells. In contrast, while antibodies to V3 loop peptides were able to neutralize virus infection of normal MM when the viral inoculum was derived from H9 cells, they were unable to do so when the viral inoculum was derived from normal T cells or MM. Antibodies present in patient sera were able to neutralize virus infection on MM regardless of the host cell of origin of the viral inoculum.

CONCLUSIONS: These results suggest that the host cell of the virus can influence the neutralizing capacity of antibodies against an immunodominant domain of gp120. They also bring into question the practice of using only neoplastic cells as either a source of viral inoculum or as target cells in neutralization assays.

Keywords: AEGIS, HIV Infections, Antibodies, Anti-Idiotypic, HIV Antibodies, HIV Envelope Protein gp120, HIV, HIV Antigens, Acquired Immunodeficiency Syndrome, Peptides, Deltaretrovirus, Immune Sera, Human, virology, ICA7KWDaegis,hivinfections,antibodies,anti-idiotypic,hivantibodies,hivenvelopeproteingp120,hiv,hivantigens,acquiredimmunodeficiencysyndrome,peptides,deltaretrovirus,immunesera,human,virology,ica7
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MA76

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.