AEGiS-07IAC: Ability of antisera raised to synthetic peptides representing the V3 region of different HIV-1 isolates to neutralize infection of both primary monocytes/macrophages and T-cell lines.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

Print this Article


Ability of antisera raised to synthetic peptides representing the V3 region of different HIV-1 isolates to neutralize infection of both primary monocytes/macrophages and T-cell lines.

Int Conf AIDS 1991 Jun 16-21; 7:103 (abstract no. M.A.1047)
Butto S, McDanal CB, Greenwell TK, Streilein AF, Langlois AJ, Bolognesi DP, Matthews TJ; Department of Surgery, Duke University Medical Center, Durham, NC 22710

The pathogenesis of AIDS remains incompletely understood. Although HIV causes a cytocidal infection of CD-4 bearing cells in vitro, this phenomenon alone is insufficient to fully explain the immunologic dysfunction associated with AIDS. In particular, the role of monocytes/macrophages is poorly understood.

OBJECTIVE: To study the role of the variable V3 region from the viral glycoprotein gp120 in the infection of monocytes/macrophages in vitro. Antibodies directed at this region are known to block infection of T-cell lines with high efficiency. The mechanism of this neutralization process is not clear, although it is thought to occur after the CD4/gp120 binding. The major objective of this study is to test if V3 antibodies display similar blocking activity for the infection of macrophages.

METHODS: We synthesized peptides 20 to 25 residues in length containing sequences from the V3 region of several field isolates as well as the monocyte-tropic HTLV-IIIBa-L strain. Some of these peptides were used to immunoaffinity purify a number of human HIV-1 positive sera. RESULTS AND DISCUSSION: Each of these isolates is able to infect productively both T-lymphocytes and monocytes/macrophages. The role and effect of guinea pig antibodies to the synthetic peptides, as well as immunoaffinity purified human antibodies, in neutralizing the infection by these "dual tropic" isolates on both primary monocytes/macrophages as well as T-cells, will be discussed.

Keywords: AEGIS, HIV-1, Monocytes, Macrophages, T-Lymphocytes, Immune Sera, Cell Line, Antigens, CD4, Peptides, Acquired Immunodeficiency Syndrome, HIV-1 Reverse Transcriptase, Infection, Human, In Vitro, ICA7KWDaegis,hiv-1,monocytes,macrophages,t-lymphocytes,immunesera,cellline,antigens,cd4,peptides,acquiredimmunodeficiencysyndrome,hiv-1reversetranscriptase,infection,human,invitro,ica7
910616
MA1047

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.