AEGiS-07IAC: HIV infection of a cell line derived from novel gastrointestinal T-lymphocyte population (CD4-, CD8-, TCR alpha beta +).

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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HIV infection of a cell line derived from novel gastrointestinal T-lymphocyte population (CD4-, CD8-, TCR alpha beta +).

Int Conf AIDS 1991 Jun 16-21; 7:103 (abstract no. M.A.1045)
Toshio H, Maeda Y, Maeda M, Takatsuki K; Kumamoto University Medical School, Kumamoto, Japan

OBJECTIVE: Dysfunctions of local immunity are presumed to be prerequisite for systemic immunodeficiency. To obtain clues for the mechanisms of immunodeficient states in HIV infected individuals, we have asked whether a T cell line derived from novel T-lymphocyte population in the gastrointestinal tract could be infected with HIV or not.

METHODS: IL-2 dependent cell line (43/T) was established from a patient with adult T cell leukemia involving the epithelium of the gastrointestinal tract (Lancet in press). The cell line harbored unique cell surface markers of the original leukemia cells, namely, CD4-, CD8-, bearing T-cell receptor alpha beta heterodimer. 43/T cells were inoculated with HTLV-III MN followed by the cultivation. Culture supernatants were assayed for p24 antigen, and the infection was also confirmed by the use of mAbs; VAK5 (alpha-p24) and mu39.1 (alpha-V3 of HTLV-III MN).

RESULTS: P24 antigen was detected in the culture supernatants 40 days after infection. Thereafter, the infected cell line continuously produced viral antigen for more than 3 months. Over 96% of the infected cells were positive for both VAK5 and mu39.1 mAbs. Moreover, syncytia formation of infected cells co-cultured with uninfected Molt-4 Clone 8 cells was observed.

CONCLUSIONS: Infection of this novel subset by HIV-1 may explain immunodeficient states of the gastrointestinal tract in HIV infected individuals and would be useful to analyze the mechanisms for CD4-independent HIV-1 infection.

Keywords: AEGIS, Antigens, CD8, Antigens, CD4, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, HIV Infections, HIV-1, Cell Line, HIV, Antigens, CD, Leukemia-Lymphoma, T-Cell, Acute, HTLV-I-Associated, HIV Core Protein p24, Leukemia, T-Cell, HIV Antigens, CD9 antigen, Adult, Human, ICA7
910616
MA1045

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