AEGiS-07IAC: Human diploid fibroblasts and epithelial cells pre-treated with interferon gamma present increased HIV adsorption.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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Human diploid fibroblasts and epithelial cells pre-treated with interferon gamma present increased HIV adsorption.

Int Conf AIDS 1991 Jun 16-21; 7:102 (abstract no. M.A.1040)
Dolei A, Serra C, Arca MV, Toniolo A; Institute of Microbiology and Virology, University of Sassari, Italy

OBJECTIVE: To correlate the permissiveness to HIV of adherent cells to the expression of surface molecules.

METHODS: HIV-1 (strain HTLV-IIIB) was given to human diploid lung embryo fibroblasts and to other adherent human cell lines, either untreated or pre-treated with recombinant interferon (IFN) gamma. Modulation of membrane molecules was evaluated by radiobinding to living cells of a panel of MAbs directed against membrane markers, or by immunofluorescence. HIV infection was monitored by syncytium assays, RT activity and p24 ELISA.

RESULTS: Pre-treatment of cells with IFNgamma increased the surface expression of Class I, beta2-microglobulin, and CD4 molecules, and induced de novo Class II DR and DP antigens; HIV adsorption was increased by a factor of 2-4. Virus yield was reduced only in those cells which completed virus cycle within 2-3 days. Infectivity of adsorbed particles lasted longer in IFN-treated cells, suggesting delayed uncoating. Newly formed p24 and infectious virus were detected 48-72 hrs p.i. in continuous cell lines. Diploid fibroblasts started to produce low levels of HIV only 20-30 days p.i., which were not influenced by pre-treatment with IFNgamma, whereas physiological doses of TNF enhanced p24 production. No matter of pre-exposure to IFN, persistent infection with HIV could be established in diploid fibroblasts, with chronic production of HIV antigens and low levels of infectivity.

Keywords: AEGIS, Interferon Type II, HIV-1, Fibroblasts, Antigens, CD4, Epithelial Cells, HIV Antigens, HIV Infections, Tumor Necrosis Factor, Cell Line, Deltaretrovirus, Giant Cells, Enzyme-Linked Immunosorbent Assay, Human, ICA7KWDaegis,interferontypeii,hiv-1,fibroblasts,antigens,cd4,epithelialcells,hivantigens,hivinfections,tumornecrosisfactor,cellline,deltaretrovirus,giantcells,enzyme-linkedimmunosorbentassay,human,ica7
910616
MA1040

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