AEGiS-07IAC: Preferential susceptibilities of adherent cells derived from human brains to an HIV-1 mutant and HIV-2 and SIV isolates.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

Print this Article


Preferential susceptibilities of adherent cells derived from human brains to an HIV-1 mutant and HIV-2 and SIV isolates.

Int Conf AIDS 1991 Jun 16-21; 7:100 (abstract no. M.A.1035)
Takeuchi Y, Hoshino H, Miura T, Hayami M, Clapham PR, Weiss RA; Gunma University, Maebashi, Gunma, Japan

We have been studying a mutant HIV-1, which is named GUN-1V and can productively infect fibroblastoid cells (BT cells) derived from human brains by a CD4-mediated mechanism. BT cells seem to originate from normal blood vessel cells. Proto-type HIV-1 isolates cannot replicate well in BT cells. The genetic difference in cell tropism between GUN-1V and its parental, proto-type isolate, GUN-1WT, has been shown to be a single base exchange in V3 region in the env gene. In this study, we compared the cell tropism of GUN-1V with those of various immunodeficiency viruses including HIV-2s and SIVs.

METHODS: HIV-1 isolates (IIIB, LAV-1, ARV, RF, Z84, GUN-1WT and GUN-1V), HIV-2 isolates (GH-1, LAV-2 and SBL6669) and SIV isolates (AGM, MND and MAC) were examined. CD4-positive HeLa (HeLa-CD4+) and U87 glioma (U87-CD4+) cells, BT cells and M8166, MT-4 and MOLT-4 T lymphocytes were cocultivated with persistently infected cells or inoculated with virus stocks. Virus replication was examined by syncytium formation, reverse transcriptase assay and immunofluorescence. RESULTS AND DISCUSSION: All virus isolates infected M8166, MT-4 and MOLT-4 cells. IIIB, LAV-1, RF, GUN-1WT, GUN-1V, LAV-2 and SBL6669 isolates, but not others, efficiently replicated and induced syncytia in HeLa-CD4+ cells. GUN-1V and all of HIV-2 and SIV isolates infected both of BT and U87-CD4+ cells, whereas replication of HIV-1 isolates other than GUN-1V was not detected. These results suggest that brain-derived cells, BT and U87-CD4+ cells, might have similar mechanism(s) of virus reception which is available to GUN-1V, HIV-2s and SIVs, but not to proto-type HIV-1s.

Keywords: AEGIS, HIV-2, SIV, HIV-1, Antigens, CD4, Virus Replication, Genes, env, Giant Cells, Brain, Human, virology, genetics, ICA7KWDaegis,hiv-2,siv,hiv-1,antigens,cd4,virusreplication,genes,env,giantcells,brain,human,virology,genetics,ica7
910616
MA1035

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.