Genomic variability of HTLV-1 according to geographical areas and associated pathology.
Int Conf AIDS 1991 Jun 16-21; 7:98 (abstract no. M.A.1025) Komurian F, Pelloquin F, Giordano C, Gessain A, Sonoda S, de-The G; Merieux Institute, Marcy l'Etoile, France
In order to study the geographical and disease-associated variations of the HTLV-I genome, the ex-vivo DNA from nine patients were studied by PCR, followed by direct DNA sequencing. For each viral strain, about 2,000 bp were thus sequenced, including LTR, env gene, px II, px III and px IV coding frames of the px region. The genomic variations observed in the LTR region were significantly higher than those seen in other viral genes. In the px region, very few mutations were present in px II and px III genes, but px IV exhibited numerous single point mutations. No specific mutation could be linked to ATL or PST/HAM specific pathology. Observed changes among HTLV-I from different geographical areas (Ivory Coast, Caribbean area or Japan) suggest that some Japanese HTLV-I may be close to Caribbean HTLV-I, while others are independent. African HTLV-I appear to represent a group apart.
Keywords: AEGIS, Human T-lymphotropic virus 1, Paraparesis, Tropical Spastic, Genes, pX, Genes, env, Terminal Repeat Sequences, Genes, Viral, Polymerase Chain Reaction, DNA, Mutation, Sequence Analysis, DNA, DNA Restriction Enzymes, Caribbean Region, Cote d'Ivoire, Japan, Human, pathology, genetics, ICA7
910616
MA1025
