Dual tropism for lymphocytes and macrophages is a common feature of all HIV isolates.
Int Conf AIDS 1991 Jun 16-21; 7:93 (abstract no. M.A.1006) Valentin A, Fredriksson R, Albert J, Fenyo EM, Asjo B; Depts. of Virology, Karolinska Institute and National Bacteriological Laboratory, Stockholm, Sweden
AIM OF THE STUDY: The aim of the study was to investigate whether HIV isolates show any difference in the capacity to infect and replicate in peripheral blood leukocytes (PBL) and blood derived macrophages (BDM). MATERIAL AND METHODS: PBL and BDM obtained from HIV seronegative healthy blood donors were infected with 50 HIV-1, 12 HIV-2 primary isolates and 8 molecular clones (MC) derived from HIV-1 isolates. In addition, the HIV-1 isolates IIIB and BaL, considered as prototype lymphotropic and macrophagetropic viruses respectively, were included. Viral replication was monitored by reverse transcriptase (RT) activity and presence of p24 antigen in culture supernatants. Occasionally, when viral replication was undetectable, cocultivation experiments with PHA-stimulated PBL were done.
RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME.
CONCLUSION: All HIV isolates, including HTLVIIIB and HIV-1BaL, are tropic for both T-lymphocytes and blood derived macrophages. However, they show great variations in their capacity to replicate in BDM.
Keywords: AEGIS, HIV, Macrophages, Lymphocytes, Tropism, HIV-1, Virus Replication, HIV-2, RNA-Directed DNA Polymerase, HIV Infections, HIV Core Protein p24, Receptors, HIV, T-Lymphocytes, Phytohemagglutinins, HIV Envelope Protein gp120, HIV Antigens, Anti-HIV Agents, virology, ICA7
910616
MA1006
