AEGiS-07IAC: Binding of HIV-1 gp120 protein to CD4-negative colon cancer cell lines.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

Print this Article


Binding of HIV-1 gp120 protein to CD4-negative colon cancer cell lines.

Int Conf AIDS 1991 Jun 16-21; 7:93 (abstract no. M.A.1005)
Heyworth MF, Sullivan KT, Liu GH, Kim YS; VA Medical Center and University of California, San Francisco, CA, USA

OBJECTIVE: The aims of this work were (1) to label recombinant gp120 (surface glycoprotein of HIV-1) with biotin, and (2) to compare the ability of biotinylated gp120 to bind to human lymphocytes and colon cancer cell lines.

METHODS: Recombinant glycosylated gp120 was labeled with biotin, by using sulfosuccinimidyl 6-(biotinamido) hexanoate. Binding of biotinylated (b) gp120 was then assessed by flow cytometry of lymphocytes or colon cancer cells incubated with bgp120 followed by streptavidin (SA)-labeled fluorochromes. Presence or absence of CD4 was also assessed, by flow cytometry of cells incubated with fluorochrome-labeled anti-CD4 monoclonal antibody (mAb).

RESULTS: Binding of bgp120 to CD4+ lymphocytes was observed at bgp120 concentrations of greater than or equal to 0.5mug per 10(6) cells. The bgp120 did not bind to CD4- lymphocytes. In contrast, bgp120 showed equally strong binding to CD4- and CD4+ colon cancer cells (see table). TABULAR DATA, SEE ABSTRACT VOLUME.

DISCUSSION AND CONCLUSIONS: The results suggest that bgp120 became bound to the colon cancer cells by a CD4-independent mechanism.

Keywords: AEGIS, Antigens, CD4, HIV-1, Cell Line, CD4-Positive T-Lymphocytes, Antibodies, Monoclonal, Antigens, Differentiation, T-Lymphocyte, Leu-23 antigen, Human, ICA7KWDaegis,antigens,cd4,hiv-1,cellline,cd4-positivet-lymphocytes,antibodies,monoclonal,antigens,differentiation,t-lymphocyte,leu-23antigen,human,ica7
910616
MA1005

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.