6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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In vitro proliferation of selectively stimulated HIV-1 infected T cells.

Int Conf AIDS 1990 Jun 20-23; 6:341 (abstract no. 1111)
Pichler WJ, Bettens F, Pichler CE, de Weck AL; Institute for Clinical Immunology, Inselspital Bern, Switzerland

OBJECTIVES: Selective proliferation of HIV-1 infected T cell subsets of HIV-1 infected individuals to a) expand HIV-infected cells without separation techniques and b) study regulation of HIV-1 replication in cell culture.

METHODS: PBMC of HIV-1 infected individuals were stimulated with the anti-CD3 MoAb BMAO30 F(ab)2 fragment crosslinked to a second anti-T cell MoAb (i.e. anti-CD4 or -CD8 MoAb) in presence of monocyte supernatant and IL 2. Such dual MoAb stimulation (Eur. J. Immunol. (1987) 17:873) enables to selectively stimulate only those cells (i.e. CD4+ or CD8+) which are recognized by the second MoAb. Proliferation was measured by 3H-TdR uptake. HIV-1 replication was measured by p24 enzyme immunoassay.

RESULTS: Cells of HIV-1 infected individuals responded normally to dual MoAb stimulation. The proliferation correlated to the CD4+, respective CD8+ cell number measured by immunofluorescence. Selective stimulation of CD4+ cells led in certain individuals to enhanced levels of p24 antigens. Simultaneous stimulation of CD4+ and CD8+ or CD8+ cells alone required longer in vitro cultures or inhibited detection of HIV-1 replication, emphasizing the regulatory role of stimulated CD8+ cells on HIV-1 infection.

CONCLUSIONS: Selective in vitro stimulation of HIV-1 infected PBMC is possible and enables virus amplification in culture. It seems to be a suitable tool to study regulation of HIV-1 replication.

Keywords: AEGIS, HIV-1, HIV Infections, Antigens, CD8, Antigens, CD4, Virus Replication, T-Lymphocyte Subsets, Antigens, CD3, Antigens, CD, HIV-1 Reverse Transcriptase, Monocytes, CD9 antigen, In Vitro, virology, ICA6KWDaegis,hiv-1,hivinfections,antigens,cd8,antigens,cd4,virusreplication,t-lymphocytesubsets,antigens,cd3,antigens,cd,hiv-1reversetranscriptase,monocytes,cd9antigen,invitro,virology,ica6

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