AEGiS-06IAC: The diminished erythrocyte-CR1 activity of progressive HIV infection is associated with chronic complement consumption and cytokine release.

6th International AIDS Conference

San Francisco, California, USA — June 20-23, 1990

The diminished erythrocyte-CR1 activity of progressive HIV infection is associated with chronic complement consumption and cytokine release.

Int Conf AIDS 1990 Jun 20-23; 6:336 (abstract no. 1091)
Lange M, Senderowicz A, Mohan V, B-Klein E, Inada Y; St. Luke's-Roosevelt Hospital Center, Columbia University, New York, NY, USA

OBJECTIVE: To investigate if the decreasing E-CR1 binding activity associated with progressive HIV infection is accompanied by increased consumption of alternate pathway(AP) and terminal complement cascade (TCC) as well as cytokine production.

METHODS: E-CR1 direct Coombs' for IgG & C3, complement split products (CSP: C3a desArg, SC5b-9, Bb, C5a), total hemolytic complement CH50, TNF alpha, IL-1 alpha, p24Ag and acid labile alpha-interferon(AL-INF) were measured on prospectively collected samples as follows: Gpl; control 10 Heterosexual hospital workers, HIV-. GP2; 22 Homosexual volunteers, HIV-. Gp3; 25 Homosexual volunteers, HIV+. Gp4; 24 HIV+, ambulatory clinic patients, of whom 17 had AIDS/severe ARC and 7 LAS/mild ARC.

RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. C5a and CH50 showed no statistical differences. Mean levels for C3a desArg, SC5b-9 and Bb were markedly increased in Gp 4 compared to Gps 1,2 and 3(p less than 0.005 for all 3 comparisons). Patients with LAS more often had TNF with IL-1, whereas AIDS has TNF/AL-INF/p24Ag. No cytokine combinations occurred in Gps 1,2 and 3. Triple CSP elevation occurred in 96% of Gp 4, in none of Gps 1 and 3 and one in Gp2.

CONCLUSION: Increased consumption of AP and TCC occurs in late stage of HIV infection. The accompanying elevation of TNF with or without IL-1 can result in increased HIV transactivation potentially setting up a vicious circle.

Keywords: AEGIS, Receptors, Complement 3b, Complement, HIV Infections, Cytokines, Acquired Immunodeficiency Syndrome, Erythrocytes, Interleukin-1, Complement 3a, AIDS-Related Complex, Tumor Necrosis Factor, Complement 3, Homosexuality, Complement 5a, Receptors, Complement, Glycoproteins, SC5b-9, Human, ICA6

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