6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Development of an enzyme immunoassay (EIA) using native envelope glycoprotein (gp160) for the detection of HIV antibodies.

Int Conf AIDS 1990 Jun 20-23; 6:335 (abstract no. 1085)
Nair BC, Ford G, Kalyanaraman V, Zafari M, Fang C, Gallo R, Sarngadharan M; Advanced BioScience Laboratories, Inc., Kensington, MD USA

OBJECTIVE: To evaluate the usefulness of purified native envelope antigen in HIV serodiagnosis.

METHODS: An EIA was developed using purified native gp160. This system was compared for its sensitivity and specificity with EIAs that use viral lysate and recombinant proteins as antigens.

RESULTS: The gp160 assay was determined to be highly specific as 1) 998 of 1,000 sera from healthy blood donors were negative; the 2 were confirmed positive by Western blot (WB) assay, 2) 55 sera that contain antibodies likely to produce false positive reactions were all found to be negative, 3) the results of gp160 EIA coincided with WB characteristics of 398 sera. The assay was 2-10 times more sensitive than EIAs that use virus lysate as antigen on panels of diluted sera. The assay detected antibodies in early bleeds of a seroconverter which were not detected by commercial EIAs that use solubilized virus or recombinant envelope protein as antigen.

CONCLUSION: gp160 EIA is highly specific and sensitive. The native antigen appears to be important in the earliest detection of HIV antibodies during early seroconversion. The highly purified native envelope antigen may be useful in HIV serodiagnosis.

Keywords: AEGIS, HIV Antibodies, Immunoenzyme Techniques, AIDS Serodiagnosis, Blotting, Western, Glycoproteins, Recombinant Proteins, False Positive Reactions, ICA6KWDaegis,hivantibodies,immunoenzymetechniques,aidsserodiagnosis,blotting,western,glycoproteins,recombinantproteins,falsepositivereactions,ica6

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