6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Effect of some physical and chemical treatments on reactivity of serum to HIV.

Int Conf AIDS 1990 Jun 20-23; 6:333 (abstract no. 1077)
dos Santos JI, Galvao-Castro B; Advance Laboratory of Public Health, WHO Collaborating Centre on AIDS, Fundacao Oswaldo Cruz, Salvador, Bahia, Brasil

OBJECTIVE: To evaluate the effects of some physical and chemical manipulations employed for storage and preservation of serum samples, on the HIV reactivity.

METHODS: The sera (10 positive and 5 negative) were subjected to the following procedures: heating at 56 degrees C during one hour; freeze-thawing; freeze-drying; glycerol (50%); sodium azide (0.1%); thymerosal (0.01%); triton X-100 (1%) and chloroform. The sera were then assayed in an indirect (Organon) and competitive (Behring) ELISA.

RESULTS: The use of glycerol and sodium azide induced false positive reactions in competitive ELISA (p greater than 0.05). In addition, sodium azide, thymerosal and triton X-100 reduced the reactivity of positive sera in indirect ELISA (p greater than 0.05), but without inducing false negative results.

CONCLUSIONS: Although the majority of procedures usually intended for preservation and storage of serum can be used with little or no loss of HIV specific reactivity, the use of both glycerol and sodium azide should be avoided in sera to be tested in competitive ELISA.

Keywords: AEGIS, HIV, HIV Antibodies, Enzyme-Linked Immunosorbent Assay, HIV Seropositivity, HIV Infections, HIV Antigens, False Positive Reactions, HIV Seronegativity, Freeze Drying, methods, immunology, ICA6KWDaegis,hiv,hivantibodies,enzyme-linkedimmunosorbentassay,hivseropositivity,hivinfections,hivantigens,falsepositivereactions,hivseronegativity,freezedrying,methods,immunology,ica6

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