6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

Print this Article


Cytotoxic responses in mice immunized with SIVMAC251 vaccinia virus (VV) recombinants.

Int Conf AIDS 1990 Jun 20-23; 6:327 (abstract no. 1055)
Rappocciolo G, Maltos DL, Johnson PR, Chanh TC; Southwest Foundation for Biomedical Research, San Antonio, TX, USA

OBJECTIVE: To study the SIV-specific cytotoxic response in mice immunized with recombinant VV expressing SIV envelope protein or internal core gag protein.

METHOD: BALB/c mice were inoculated intravenously with VV recombinants and boosted 3 times at 2 wk. intervals. Cytotoxicity was performed using syngenic P815 or allogenic BW5147 target cells infected with VV expressing env or gag protein. Mice were sacrificed 2 wk. after the last boost and spleen cells were cultivated in the presence of recombinants VV infected syngenic spleen cells and interleukin 2 (IL2) for 7 d and then used as effectors in a 51Cr release cytotoxicity assay.

RESULTS: 1. SIV-specific CD8(+) T lymphocytes were detected with P815-vv-env or P815-vv-gag target cells after in vitro expansion in the presence of antigen presenting cells (APC) and IL2. 2. The cytotoxicity was H-2 class I restricted since no cytotoxicity was obtained with mismatched targets (BW5147) thus also excluding a participation of NK cells in the phenomenon observed. 3. Antigen-specific T cell lines were obtained after repeated in vitro stimulation with syngenic APC VV-env or gag infected and IL2. 4. Target cells infected with VV expressing HIV-1 env protein were not lysed by the SIV-specific effectors.

CONCLUSIONS: Both the external env protein and the internal gag protein of SIV can induce cell mediated immune responses in mice. This information may be valuable in the development of experimental vaccines against HIV infection.

Keywords: AEGIS, Vaccinia virus, Gene Products, env, Gene Products, gag, SIV, HIV-1, T-Lymphocytes, Interleukin-2, Antigens, CD8, HIV Infections, Mice, Animal, In Vitro, ICA6KWDaegis,vacciniavirus,geneproducts,env,geneproducts,gag,siv,hiv-1,t-lymphocytes,interleukin-2,antigens,cd8,hivinfections,mice,animal,invitro,ica6

900620
1055

Copyright © 1990 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.