6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Evidence for the protein synthesis directed by the HIV 1 antisense proviral DNA strand.

Int Conf AIDS 1990 Jun 20-23; 6:325 (abstract no. 1045)
Goltsov V, Blinov V, Sukhanova L, Zaitsev I; Institute for Viral Preparations, Moscow, USSR

OBJECTIVE: Determination whether the open reading frames (ORFs) of the "A" (antisense) HIV 1 proviral DNA strand can express proteins in vivo.

METHODS: Molecular cloning for recombinant polypeptide producing, human sera screening by ELISA, computer analysis of nucleotide and amino acid sequences.

RESULTS: There are several conserved short ORFs within antisense proviral DNA strand of HIV 1. The DNA fragments of 2 longest ORFs sequences were cloned in the E.coli expressive vector. The recombinant proteins after isolation and purification were used in human sera testing for protein specific antibody presence by ELISA. We could find sera positively reacted with recombinant proteins only among samples from seropositive HIV 1-infected persons but could not in case of seronegative persons sera. The proportion of sera from HIV 1-infected persons giving strong signals in ELISA varied within 2-10% range and was approximately similar for both AIDS patients and non-symptomatic persons sera groups. Computer analysis showed the presence of several splicing sites between the ORFs that permitted the combined amino acid chain formation from both ORF nucleotide sequences. All putative proteins are very hydrophobic and resemble membrane associated structures.

CONCLUSION: The ORF sequences located within HIV 1 antisense proviral DNA strand appear to be expressed in vivo.

Keywords: AEGIS, Resin Cements, Open Reading Frames, HIV-1, Acquired Immunodeficiency Syndrome, Cloning, Molecular, Recombinant Proteins, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Direct, Human, genetics, ICA6KWDaegis,resincements,openreadingframes,hiv-1,acquiredimmunodeficiencysyndrome,cloning,molecular,recombinantproteins,enzyme-linkedimmunosorbentassay,escherichiacoli,direct,human,genetics,ica6

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