6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Demonstration by in situ hybridization of HIV1 gag-pol provirus transcription in peripheral blood mononuclear cells of a patient with a negative antigenaemia.

Int Conf AIDS 1990 Jun 20-23; 6:320 (abstract no. 1026)
Delord B, Pellegrin JL, Fleury HJ; Centre Hospitalier Regional et Universite de Bordeaux II, Bordeaux, France

OBJECTIVE: To investigate the transcription of HIV1 provirus in Peripheral Blood Mononuclear Cells (PBMC) of a patient with a negative antigenaemia.

METHODS: Patient of stage II of CDC with a negative HIV1 antigenaemia (HIV1 Ag) as determined by Abbott Enzyme Immunosorbent Assay after treatment of the sample with Triton. PBMC of the patient were obtained from heparinised blood sample after centrifugation, then spread out on glass-slides with a Shandon cytocentrifuge and in situ hybridized with a [35S] labeled single stranded RNA probe specific for gag-pol (nucleotides 225 to 3140) of LAVBRU HIV1 allowing the detection of viral and/or messenger corresponding RNA.

RESULTS: Positive cells could be observed; although they were rare, they are consistent with an active expression of HIV1 gag-pol provirus even in a patient with a negative HIV1 Ag.

CONCLUSION: Transcription of HIV1 provirus in PBMC of a patient with a negative HIV1 Ag can be demonstrated by in situ hybridization.

Keywords: AEGIS, Proviruses, Transcription, Genetic, RNA Probes, RNA, Messenger, RNA, Antisense, DNA Primers, Human, genetics, ICA6KWDaegis,proviruses,transcription,genetic,rnaprobes,rna,messenger,rna,antisense,dnaprimers,human,genetics,ica6

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