5th International AIDS Conference Montreal, Quebec, Canada — Jun 4-9, 1989

Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.544)

Pathouli C, Spiliotopoulou J, Kontopoulou-Griva I; Athens University, Athens, Greece

OBJECTIVE: We observed that a small part of normal PBL 8-14% forms spontaneous rosettes (R) with their AE in Man. We wanted to investigate this phenomenon in individuals infected by HIV. We studied 23 Hemophiliacs positive for HIV and 22 individuals infected by HIV and/or suffering from AIDS.

METHODS: PBL were separated according to classic techniques. AE suspensions 1% were prepared and the mixture (V/V) were observed by microscopy after incubation for 24 hours in 4 degrees C temperature.

RESULTS: We found AELFR from 50% till 97% and we were able to inhibit the R formation by using monoclonal antibodies (MA) from "Dakopatts": T1, T2, T4, T8, B after addition of them into the mixture. The inhibition was strong by T-MA and very low by B-MA except for one case: a patient with Coombs positive hemolytic anemia (A).

CONCLUSIONS: We may until now conclude that a small numeral of PBL are able to attack against their AE in order to eliminate old or altered E from blood stream. Probably this PBL subpopulation may increase in pathologic states like HIV infection so as to become dangerous for normal AE. It is possible this mechanism is partially responsible for the A, which A develops later in such patients.

Keywords: AEGIS, Rosette Formation, Lymphocytes, Erythrocytes, Acquired Immunodeficiency Syndrome, HIV Infections, Temperature, Human, Male, pathogenicity, blood, ICA5

1989-06-04
B544

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