4th International AIDS Conference Stockholm, Sweden. — June 12-16, 1988

Int Conf AIDS. 1988 Jun 12-16;4:1.117 (abstract no. 1020)

E Bohnlein, JW Lowenthal M Siekevitz, BR Franza^* and WC Greene
Howard Hughes Med. Inst., Duke Univ., Durham, NC 27710; ^*CSH Lab., Cold Spring Harbor, NY 11724.

A variety of mitogens activate both the HIV-1 LTR and the interleukin-2 receptor (Tac) promoter. Sequences required for mitogen induced IL-2 receptor promoter activation in different T cells have been defined by deletion mutagenesis. The specific interaction of inducible trans-acting factors with these functionally defined sequences has been demonstrated. Using mutated oligonucleotides we have mapped the binding site of one of the factors to a 12 bp segment (-267 to -256). This IL-2 receptor promoter sequence was found to share striking sequence homology with.tne transcriptional enhancer Of the HIV-1 virus. Competition experiments revealed cross-inhibitory effects of these two regulatory sequences for the binding of inducible nuclear proteins. The binding of a 86 kD inducible protein to both transcriptional elements was directly demonstrated using biotinylated oligonucleotides and ³⁵S-methionine labeled nuclear extracts in a newly developed microscale DNA-affinity precipitation assay. Thus, while activation of the IL-2 receptor and HIV-1 LTR leads to opposing effects on T cell growth, these diverse responses are initially regulated by the same inducible transcription factor.

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