1st National Conference Human Retroviruses and Related Infections Washington, DC - December 12-16, 1993

Natl Conf Hum Retrovir Relat Infect 1993 Dec 12-16;1: (abstract no. 15)

Li J¹, Lord C², Letvin N², Sodroski J^1
1The Dana-Farber Cancer Institute, Boston, MA; ²New England Regional Primate Research Center, Southborough, MA

We have previously shown that 7000 TCID₅₀ of SHIV, a hybrid virus that contains the gag-pol of SIVmac239 and the env of HIV-1 (HXBc2), could infect four cynomolgus macaques intravenously. The animals were positive for virus isolation for 1 to 2 months post-infection. Thereafter, the animals became variable for virus isolation, although virus had been isolated from one animal after one year of infection. All four monkeys had sustained anti-HIV-1 env antibodies through at least a year post-infection. At least one of these animals produced a strain-specific followed by a broadly neutralizing antibody response.

To date we have infected 12 out of 12 additional macaques of various species with SHIV. Data on virus isolations from of these animals will be presented.

We are using the SHIV model to study two issues: (1) Does vpu have a contribution in this animal model? (2) Does SHIV evade the immune response through env variation? Data on these issues and others will be presented.

Keywords: Animals, Genes, env, HIV-1, Macaca, genetics

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1993-12-12
15

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