AEGiS-11CROI: Full Molecular Characterization of Simian Immunodeficiency Virus Infecting Talapoins Monkeys: Evidence for a New SIV Lineage in Miopithecus Species.

11th Conference on Retroviruses and Opportunistic Infections


San Francisco, California - February 8 - 11, 2004

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Full Molecular Characterization of Simian Immunodeficiency Virus Infecting Talapoins Monkeys: Evidence for a New SIV Lineage in Miopithecus Species.

Conf Retrovir Opportunistic Infect 2004 Feb 8-11; 11:(abstract no. 379)

Courgnaud V, Liegeois F, Switzer WM, Loul S, Mpoudi-Ngole E, Delaporte E, Peeters M; UR36, Inst. of Res. and Devt. and Univ. of Montpellier I, France

BACKGROUND: Primate lentiviruses are a diverse group that naturally infect at least 33 nonhuman African primate species. Initially, 6 distinct lineages (SIVcpz, SIVsm, SIVagm, SIVsyk, SIVlhoest, and SIVcol) have been described. More recently, a number of potential recombinant lineages have been also described highlighting the increasing complexity of phylogenetic relationships among primate lentiviruses. Here we report the full-length and partial sequences of SIVtal strains from two talapoins monkeys (Miopithecus oguensis) wild-caught in Cameroon and from 4 captive animals obtained from different U.S. zoos.

METHODS: By PCR we amplified viral pol sequences (650 bp) from uncultured PBMC DNA from these animals and for one willd-caught animal, we obtained the entire genome (SIVtal266) using long PCR method by targeting unintegrated circular DNA. All sequences were compared to previously reported SIV sequences. Phylogenetic tree analyses of pol sequences showed that all viruses from talapoins monkeys cluster together forming a monophyletic species-specific SIVtal lineage and thus indicating that these monkeys are the natural hosts for SIVtal. Full-length genome analysis revealed that SIVtal266 had a genomic organization characteristic for most primate lentiviruses (i.e. lacking vpu and vpx). While, overall, the SIVtal lineage was more closely related to the recently described SIVgsn lineage present in greater spot-nosed, mustached and mona monkeys in Cameroon and to the SIVsyk lineage. Using a neighbor-joining method, the clustering of these 3 lineages was significant in Gag and Pol trees as well as in the Env tree when excluding the SIVcpz lineage which has been recently shown to be recombinant between SIVgsn and SIVrcm.

CONCLUSIONS: The geographic range of the gabon talapoin (Miopithecus oguensis) is from the Cameroon, south of the River Sanaga, and Rio Muni, Gabon. Although talapoin monkeys belong to the Miopithecus genus while Syke's, greater spot-nosed, mustached and mona monkeys are from Cercopithecus species, these data suggest that SIVtal, SIVsyk, and SIVgsn/mon/mus evolved from a common ancestor.

Keywords: AEGIS, SIV, Cercopithecus, Cercopithecinae, Polymerase Chain Reaction, Cameroon, Gabon, Equatorial Guinea, United States, AnimalKWDaegis,siv,cercopithecus,cercopithecinae,polymerasechainreaction,cameroon,gabon,equatorialguinea,unitedstates,animal

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Copyright © 2004 - Foundation for Retrovirology and Human Health. Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed (AIDSLINE) from National Library of Medicine.