Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
Lymphocytes migrate from the blood into the bronchoalveolar lavage and lung parenchyma in the asthma model of the brown Norway rat.
Am J Respir Crit Care Med. 2000 Feb;161(2 Pt 1):558-66. Unique Identifier : AIDSLINE MED/20180269 Schuster M; Tschernig T; Krug N; Pabst R; Centers of Anatomy, Anesthesiology and Pneumology, Medical School; of Hanover, Hanover, Germany.
Abstract:
Lymphocyte migration from the blood into the lung has been suggested as being responsible for the increase of lymphocytes, in particular CD4 T cells, in the bronchoalveolar lavage (BAL) and bronchial mucosa in human asthma, but so far there has been no direct proof. We studied lymphocyte immigration and lymphocyte subpopulations in three lung compartments in ovalbumin (OVA)-sensitized and -challenged brown Norway (BN) rats. Increased numbers of CD4 and interleukin 2 (IL-2) receptor-positive T cells were found in the BAL and lung parenchyma in treated animals, but also increased numbers of CD8 T cells, B cells, and natural killer (NK) cells. For direct proof of lymphocyte migration from the blood into the lung, leukocytes were labeled with a fluorescent dye, 5- (and 6-) carboxyfluorescein-diacetate-succinimidyl-ester (CFSE), and injected intravenously immediately prior to OVA aerosol challenge. One day after challenge the number of CFSE(+), i.e., newly immigrated lymphocytes, was determined by flow cytometry gated on the lymphocyte cluster. A 15 times (1.5 times) higher number of CFSE(+) lymphocytes was found in the BAL (the lung parenchyma) of treated animals in comparison with control rats. In the BAL 51.8% of CFSE(+) cells were CD4-positive (parenchyma 72.7%) and 29.4% IL-2 receptor-positive (parenchyma 34.2%). There was no difference whether the leukocytes for labeling and injection were obtained from untreated or from OVA-sensitized donor animals. Our data show that lymphocyte immigration is at least in part responsible for the increase in lymphocyte numbers in the BAL and lung parenchyma in this animal asthma model.
Keywords: JOURNAL ARTICLE Animal Asthma/*IMMUNOLOGY/PATHOLOGY Bronchoalveolar Lavage Fluid/*IMMUNOLOGY Cell Movement/*IMMUNOLOGY CD4 Lymphocyte Count Disease Models, Animal Human Lung/*IMMUNOLOGY/PATHOLOGY Lymphocytes/*IMMUNOLOGY/PATHOLOGY Male Rats Rats, Inbred BN Receptors, Interleukin-2/IMMUNOLOGY Support, Non-U.S. Gov't
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Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
