Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
Variability and immunogenicity of human immunodeficiency virus type 1 p24 gene quasispecies.
Clin Diagn Lab Immunol. 2000 May;7(3):377-83. Unique Identifier : AIDSLINE MED/20261943 Iroegbu J; Birk M; Lazdina U; Sonnerborg A; Sallberg M; Divisions of Clinical Virology, F68, Karolinska Institute,; Huddinge University Hospital, S-141 86 Huddinge, Sweden.
Abstract:
Despite the conserved nature of the human immunodeficiency virus type 1 (HIV-1) gag gene, multiple quasispecies of the p24 gene coexist in HIV-1-infected patients. We cloned and sequenced 31 p24 genes from four HIV-1-infected patients. The intrapatient homology between the p24 genes ranged from 97.1 to 99.1%, whereas the interpatient homology ranged from 91.5 to 93.8%, suggesting a host-specific evolution. Synonymous and nonsynonymous nucleotide changes were evenly distributed in the p24 gene, with 27 and 28%, respectively, located within host human leukocyte antigen class I recognition sites. This would suggest only a minor influence from the host cytotoxic T-cell response on the evolution of the p24 gene. The importance of minor variations within p24 was analyzed by designing DNA-based immunogens from two distinct p24 quasispecies genes simultaneously derived from one patient. In plasmid-immunized H-2(b), H-2(d), and H-2(k) haplotype mice, a clear influence from the host major histocompatibility complex was noted on the immune responses, fully consistent with those noted when a recombinant p24 protein is used as the immunogen. The two p24 DNA immunogens did not differ in their immunogenicity, indicating that the limited genetic variability (<1%) had little influence on the immune responses.
Keywords: JOURNAL ARTICLE Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Adult Animal Binding Sites/IMMUNOLOGY Cloning, Molecular Gene Expression Regulation, Viral/IMMUNOLOGY Histocompatibility Antigens Class I/IMMUNOLOGY Human HIV Core Protein p24/CHEMISTRY/*GENETICS/*IMMUNOLOGY HIV-1/*GENETICS/*IMMUNOLOGY Male Mice Mice, Inbred BALB C Mice, Inbred CBA Mice, Inbred C57BL Molecular Sequence Data Phylogeny Plasmids Recombinant Proteins/GENETICS/IMMUNOLOGY Sequence Homology, Amino Acid Serologic Tests Species Specificity Support, Non-U.S. Gov't Variation (Genetics)
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Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
