Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency.

Can J Vet Res. 2000 Apr;64(2):101-6. Unique Identifier : AIDSLINE MED/20262998
Simard C; Richardson S; Dixon P; Belanger C; Maxwell P; Retrovirology Centre of Expertise, Canadian Food Inspection; Agency, Charlottetown, Prince Edward Island. simardc@em.agr.ca

Abstract: Four commercially available bovine leukemia virus (BLV)-ELISA kits from Europe or the United States were compared to the agar gel immunodiffusion (AGID) test officially approved by the Canadian Food Inspection Agency (CFIA). A total of 1200 cattle serum samples were used. Three ELISA kits based on the envelope glycoprotein (gp51) gave an excellent correlation with the AGID test. The kappa values were 0.998, 0.984, and 0.986 for the ELISA kits #1, #2, and #3, respectively. The ELISA kit based on the p24 core protein was found to be less sensitive than the officially approved AGID test and detected 5.13% of false negatives. Forty BLV AGID strongly positive serum samples were diluted. Based on the dilution experiment, the gp51 ELISA kits were found to be more sensitive than the AGID test kits. They were capable of detecting antibodies in samples diluted up to 1/5000 (kit #1), 1/20 800 (kit #2) and 1/4000 (kit #3), whereas the AGID kit was only capable of detecting antibodies in samples diluted up to 1/100. Based on these observations, the gp51 BLV-ELISA was recognized as an official test method for the serodiagnosis of bovine leukosis in Canada.
Keywords: JOURNAL ARTICLE Agar Animal Antibodies/ANALYSIS/IMMUNOLOGY Cattle Cattle Diseases/*DIAGNOSIS/IMMUNOLOGY Comparative Study Enzootic Bovine Leukosis/*DIAGNOSIS/IMMUNOLOGY Enzyme-Linked Immunosorbent Assay/METHODS/STANDARDS/VETERINARY Immunodiffusion/METHODS/STANDARDS Leukemia Virus, Bovine/*IMMUNOLOGY Sensitivity and Specificity Serologic Tests/METHODS

KWDjournalarticleagaranimalantibodies/analysis/immunologycattlecattlediseases/KWDdiagnosis/immunologycomparativestudyenzooticbovineleukosis/KWDdiagnosis/immunologyenzyme-linkedimmunosorbentassay/methods/standards/veterinaryimmunodiffusion/methods/standardsleukemiavirus,bovine/KWDimmunologysensitivityandspecificityserologictests/methods
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