PCR detection of host and HIV-1 sequences from archival brain tissue.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

PCR detection of host and HIV-1 sequences from archival brain tissue.

J Neurovirol. 2000 Apr;6(2):164-71. Unique Identifier : AIDSLINE MED/20284038
Smith KM; Crandall KA; Kneissl ML; Navia BA; Program in Genetics, Tufts University, Sackler School of; Biological and Biomedical Sciences, 136 Harrison Avenue, Boston,; Massachusetts, MA 02111, USA.

Abstract: Mutations in CCR5 and CCR2b have been recently shown to affect disease progression towards AIDS. A role for these host genotypes in AIDS dementia complex (ADC) has also been postulated but remains unclear. Additionally, brain-derived envelope sequences from HIV-1 have been associated with ADC but their specific contribution to pathogenesis remains uncertain. This study demonstrates the successful use of PCR techniques to isolate host CCR5 and CCR2b, and HIV-1 V3 sequences from paraffin embedded tissues from patients with and without ADC. PCR amplification from archival tissue offers a novel approach for studying the interactions between potential neuroprotective elements in the host and virulence determinants in HIV that may contribute to differences in susceptibility to ADC.

Keywords: JOURNAL ARTICLE Amino Acid Sequence AIDS Dementia Complex/METABOLISM/VIROLOGY Basal Ganglia/CHEMISTRY/VIROLOGY Brain/*VIROLOGY *Brain Chemistry Comparative Study Disease Progression DNA/*ANALYSIS/GENETICS/ISOLATION & PURIF DNA, Viral/GENETICS/ISOLATION & PURIF Evolution, Molecular Frontal Lobe/CHEMISTRY/VIROLOGY *Genes, env Genetic Predisposition to Disease Genotype Human HIV Envelope Protein gp120/*GENETICS HIV-1/CLASSIFICATION/GENETICS/*ISOLATION & PURIF Molecular Sequence Data Paraffin Embedding Peptide Fragments/*GENETICS Phylogeny Polymerase Chain Reaction/*METHODS Proviruses/GENETICS Receptors, Cytokine/*GENETICS Receptors, CCR5/*GENETICS Sequence Alignment Sequence Homology, Amino Acid Spleen/CHEMISTRY/VIROLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Visual Cortex/CHEMISTRY/VIROLOGY
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A00A1091

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