Role of T cell subsets in the modulation of Mycobacterium avium growth within human monocytes.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Role of T cell subsets in the modulation of Mycobacterium avium growth within human monocytes.

Cell Immunol. 2000 May 25;202(1):6-12. Unique Identifier : AIDSLINE MED/20334540
Shiratsuchi H; Krukovets I; Ellner JJ; Case Western Reserve University, Cleveland, Ohio, 44106-4984,; USA.

Abstract: Mycobacterium avium frequently causes disseminated disease in patients with advanced AIDS with low CD4 counts. The effects of T lymphocyte on intracellular M. avium replication were examined. Plastic adherent monocytes and nonadherent lymphocytes were separated from peripheral blood mononuclear cells. After infection with M. avium, monocytes were cultured with or without autologous lymphocytes (1-10 cells/monocyte) for up to 7 days. Addition of lymphocytes to M. avium-infected monocytes significantly decreased intracellular M. avium growth after 7 days culture (n = 11, P < 0.01, paired t test) and increased IFN-gamma production compared to monocytes alone. Neutralizing IFN-gamma partially abrogated lymphocyte activity. CD4 depletion diminished anti-mycobactericidal effects and CD8(+) lymphocytes increased intracellular M. avium growth (P < 0.05, n = 5, t test). These data suggest that interactions between monocytes and nonadherent cell fractions such as CD4(+) T cells and NK cells are important in intracellular M. avium growth modulation in monocytes from healthy humans. Copyright 2000 Academic Press.

Keywords: JOURNAL ARTICLE Cell Adhesion Coculture Colony Count, Microbial CD4-Positive T-Lymphocytes/IMMUNOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY Human Interferon Type II/BIOSYNTHESIS Interleukin-2/PHARMACOLOGY Monocytes/*MICROBIOLOGY Mycobacterium avium/*IMMUNOLOGY Support, U.S. Gov't, P.H.S. T-Lymphocyte Subsets/*IMMUNOLOGY
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A00A1084

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