Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
Unique morphological alterations of the HTLV-I transformed C8166 cells by infection with HIV-1.
Pathol Oncol Res. 2000;6(1):27-37. Unique Identifier : AIDSLINE MED/20213656 Ongradi J; Laird HM; Szilagyi JF; Horvath A; Bendinelli M; National Institute of Dermato-Venerology Maria utca 41.,; Budapest, 1085, Hungary. ongjos@hotmail.com
Abstract:
C8166 cells express T lymphocyte markers, a monocyte-specific esterase, taxpolypeptide of HTLV-I. In spite of this transactivator, their HIV-1 yield is low. Their culture conditions were modified, and infected cells were immobilized on a poly-L-lysine sheet under semisolid overlays to study their phenotypic alterations and HIV-1 production by microscopy and electron microscopy. Another lymphoid cultures (MT-4, CEM, CEM-ss, AdCEM) similarly treated were infected with either HIV-1/RF or IIIB. Specificity of HIV-1 was compared to the effects of vesicular stomatitis virus (VSV). Unlike other cultures, HIV-1/RF infected C8166 cells in Eagle s MEM exhibited surface projections resembling hairy leukemia cells, which was followed by balloon degeneration and apoptosis. Immobilized HIV-1 infected cultures formed flat syncytia with several interdigitating dendritic projections. Syncytia shrunk with condensed nuclear material and axon-like filaments characteristic for infected macrophages. VSV induced enlargement and necrotic lysis of all cell types. Early postinfection with HIV-1, electron microscopy revealed irreversible membrane fusion above cell nuclei, and transient fusion between filaments. Transient presence of coated vesicles containing intact HIV-1 particles, Birbeck granule-like structures of Langerhans cells, fibrillar-lamellar structures resembling hairy leukemia or Sezary cells were detected. Late postinfection, high proportion of HIV-1 bud from polarized cytoplasm was empty particle, while that bud and entrapped in cytoplasmic vacuoles contained two or multiple cores in a fused envelope. The effect of early gene products of HIV-1 on HTLV-I and C8166 cells might elicit their latent potentials for monocyte or interdigitating dendritic cells, while in the later phase HTLV-I products might alter HIV-1 virion assembly.
Keywords: JOURNAL ARTICLE Apoptosis Calcium/PHARMACOLOGY Cell Line *Cell Transformation, Viral Cytopathogenic Effect, Viral Giant Cells/ULTRASTRUCTURE Human HIV-1/*PHYSIOLOGY HTLV-I/*PHYSIOLOGY Membrane Fusion Microscopy, Electron Microvilli/ULTRASTRUCTURE Phenotype Proviruses/PHYSIOLOGY Support, Non-U.S. Gov't T-Lymphocytes/DRUG EFFECTS/PATHOLOGY/*VIROLOGY Vacuoles/ULTRASTRUCTURE Vesicular Stomatitis-Indiana Virus/PHYSIOLOGY 001030
A00A1076
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Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
