Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
Distribution of HIV-1 subtypes among HIV-seropositive patients in the interior of Cote d'Ivoire.
J Acquir Immune Defic Syndr. 2000 Apr 15;23(5):430-6. Unique Identifier : AIDSLINE GENBANK/AF216889 Nkengasong JN; Luo CC; Abouya L; Pieniazek D; Maurice C; Sassan-Morokro M; Ellenberger D; Hu DJ; Pau CP; Dobbs T; Respess R; Coulibaly D; Coulibaly IM; Wiktor SZ; Greenberg AE; Rayfield M; Laboratory of Virology, Project RETRO-CI, Abidjan, Cote; d'Ivoire. jcn5@cdc.gov
Abstract:
Limited data exist on the distribution of HIV-1 subtypes in Cote d'Ivoire. The aim of this study is to describe the distribution of genetic subtypes of HIV-1 strains in six regions of Cote d'Ivoire. In 1997, we consecutively collected blood from 172 HIV-1-infected patients from six regional tuberculosis treatment centers. Peripheral blood mononuclear cells (PBMCs) from these people were analyzed by a restriction fragment-length polymorphism (RFLP) assay that involves a sequential endonuclease digestion of a 297-base pair polymerase chain reaction (PCR) fragment; plasma samples were tested by a V3-loop peptide enzyme immunoassay (PEIA). DNA sequencing of the protease or env genes was performed on all samples discordant in the two assays as well as a random sample of the concordant subtyped samples. Of 172 specimens, 3 were PCR-negative, and 169 were putatively classified as subtype A by RFLP. The 3 PCR-negative samples were unequivocally subtyped A by PEIA. Of the 169 RFLP subtype A samples, 159 (94%) were subtyped A by PEIA. Of the 10 discordant samples, PEIA testing classified 3 as subtype C, 2 as D, and 5 as F. Sequencing of the env gene classified these samples as 1 subtype A, 4 Ds, and 5 Gs. Thus, 163 (95%) of the specimens were subtype A, 3 subtype D, 4 subtype G, 1 A/D, and 1 A/G (IbNG) circulating recombinant forms (CRF). In conclusion, most HIV-1-infected tuberculosis patients throughout the interior of Cote d'Ivoire are infected with HIV-1 subtype A, which are very likely the A/G (IbNG) CRF. The uniform distribution of this subtype makes Cote d'Ivoire a potential site for vaccine trials.
Keywords: JOURNAL ARTICLE Adult Amino Acid Sequence AIDS-Related Opportunistic Infections/BLOOD/IMMUNOLOGY/*VIROLOGY Base Sequence Cote d'Ivoire DNA, Viral Female *Genes, env Human HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY HIV Protease/CLASSIFICATION/*GENETICS HIV Seropositivity/BLOOD/IMMUNOLOGY/*VIROLOGY HIV-1/CLASSIFICATION/*GENETICS Male Molecular Sequence Data Peptide Fragments/GENETICS/IMMUNOLOGY Phylogeny Polymorphism, Restriction Fragment Length Tuberculosis/BLOOD/IMMUNOLOGY/*VIROLOGY 001030
A00A1063
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Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.
