Expression of MHC class II in T cells is associated with increased HIV-1 expression.

Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

Expression of MHC class II in T cells is associated with increased HIV-1 expression.

Clin Exp Immunol. 2000 Aug;121(2):324-31. Unique Identifier : AIDSLINE MED/20389938
Saifuddin M; Spear GT; Chang C; Roebuck KA; Department of Immunology/Microbiology, Rush University, Chicago,; IL, USA. msaifudd@rush.edu

Abstract: HIV-1 replicates in activated T cells at significantly higher levels than in resting cells. Thus, certain molecules up-regulated during T cell activation appear to be important for HIV-1 replication. In this study, we present evidence suggesting that expression of MHC class II (class II) molecules on CD4+ T cells facilitate HIV-1 replication. T cells that expressed class II supported greater virus replication than T cells lacking class II. The class II+ cells, when either infected with HIV-1 or transfected with an env-minus HIV-1 provirus plasmid, produced 10-20-fold greater virus expression than class II- cells. Anti-class II antibody markedly inhibited virus expression in class II+ cells (but not class II- cells) and also decreased the nuclear binding activity of AP-1, an inducible transcription factor important in T cell activation and HIV-1 expression. Most importantly, the induction of class II expression by transfection of the MHC class II transactivator (CIITA) stimulated HIV-1 replication in Jurkat T cells. Taken together, these data suggest that expression of MHC class II molecules and/or CIITA in T cells enhances HIV-1 transcription.

Keywords: JOURNAL ARTICLE Cell Line Chemokines/SECRETION Comparative Study Culture Media, Conditioned/CHEMISTRY Cytokines/SECRETION CD4-Positive T-Lymphocytes/IMMUNOLOGY/SECRETION/*VIROLOGY Genes, Reporter Human HIV-1/GENETICS/*PHYSIOLOGY HLA-DR Antigens/*IMMUNOLOGY Jurkat Cells/IMMUNOLOGY/VIROLOGY Lymphocyte Transformation Proviruses/GENETICS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transcription Factor AP-1/METABOLISM Transfection *Virus Replication

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