Predominant type-2 response in infants with respiratory syncytial virus (RSV) infection demonstrated by cytokine flow cytometry. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Predominant type-2 response in infants with respiratory syncytial virus (RSV) infection demonstrated by cytokine flow cytometry.

Clin Exp Immunol. 2000 Aug;121(2):332-8. Unique Identifier : AIDSLINE MED/20389939
Bendelja K; Gagro A; Bace A; Lokar-Kolbas R; Krsulovic-Hresic V; Drazenovic V; Mlinaric-Galinovic G; Rabatic S; Department of Cellular Immunology, Institute of Immunology,; University Hospital for Infectious Diseases, University; Children's Hospital, Division of Virology, Croatian National; Institute of Public Health, Zagrab, Croatia.

Abstract: Acute RSV infection in infancy may produce some asthma-like symptoms and may be followed by a recurrent wheeze later in childhood. It has been proposed that RSV infection stimulates type-2 cytokine responses, resembling those found in atopy and asthma. Peripheral blood cells were obtained from RSV-infected infants (n = 30) and healthy controls (n = 10). After in vitro restimulation of the cells, intracellular IL-4 and interferon-gamma (IFN-gamma) were measured by flow cytometry. The cells from RSV-infected infants produced more IL-4 and less IFN-gamma than those from healthy controls. IL-4 production was more frequent in CD8 than in CD4 cells, and the bias toward IL-4 production was greatest in infants with mild infections, whereas IFN-gamma production increased with disease severity. Our conclusions are that RSV infection is associated with IL-4 production in peripheral T cells, and that peripheral blood in infants with severe disease may be depleted of cytokine-producing cells.

Keywords: JOURNAL ARTICLE Child, Preschool CD8-Positive T-Lymphocytes/IMMUNOLOGY/SECRETION Female *Flow Cytometry Human Infant Infant, Newborn Interferon Type II/*BLOOD Interleukin-4/*BLOOD Lymphocyte Transformation Male Respiratory Syncytial Virus Infections/BLOOD/*IMMUNOLOGY/VIROLOGY Respiratory Syncytial Viruses/ISOLATION & PURIF Support, Non-U.S. Gov't Th2 Cells/*IMMUNOLOGY/SECRETION

KWDjournalarticlechild,preschoolcd8-positivet-lymphocytes/immunology/secretionfemaleKWDflowcytometryhumaninfantinfant,newborninterferontypeii/KWDbloodinterleukin-4/KWDbloodlymphocytetransformationmalerespiratorysyncytialvirusinfections/blood/KWDimmunology/virologyrespiratorysyncytialviruses/isolation&purifsupport,non-uKWDsKWDgov'tth2cells/KWDimmunology/secretion
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