Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro. NLM AIDSLINE Important note: Information in this article was accurate in 2000. The state of the art may have changed since the publication date.

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Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro.

Clin Exp Immunol. 2000 Feb;119(2):299-304. Unique Identifier : AIDSLINE MED/20098469
Brodskyn C; Beverley SM; Titus RG; Laboratorio de Imunologia, Departamento de Biointeracao,; Instituto de Ciencias da Saude, Universidade Federal da Bahia,; Bahia, Brazil.

Abstract: In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-ts-) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-ts- did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the first stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect.

Keywords: JOURNAL ARTICLE Animal Cells, Cultured Comparative Study Cytokines/*BIOSYNTHESIS Human Interferon Type II/BIOSYNTHESIS Interleukin-12/PHYSIOLOGY Interleukin-5/BIOSYNTHESIS Leishmania major/ENZYMOLOGY/GENETICS/*IMMUNOLOGY/*PATHOGENICITY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Tetrahydrofolate Dehydrogenase/DEFICIENCY/GENETICS Thymidylate Synthase/DEFICIENCY/GENETICS Th1 Cells/*IMMUNOLOGY/METABOLISM/PARASITOLOGY Th2 Cells/IMMUNOLOGY/METABOLISM/PARASITOLOGY VirulenceKWDjournalarticleanimalcells,culturedcomparativestudycytokines/KWDbiosynthesishumaninterferontypeii/biosynthesisinterleukin-12/physiologyinterleukin-5/biosynthesisleishmaniamajor/enzymology/genetics/KWDimmunology/KWDpathogenicitysupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtetrahydrofolatedehydrogenase/deficiency/geneticsthymidylatesynthase/deficiency/geneticsth1cells/KWDimmunology/metabolism/parasitologyth2cells/immunology/metabolism/parasitologyvirulence
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